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Departamento de Histología, Embriología, Biología Celular y Genética, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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Departamento de Histología, Embriología, Biología Celular y Genética, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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-related diseases, such as Alzheimer’s and Parkinson’s, are related to mitochondrial dysfunction ( Chakrabarti et al. 2011 , Johri & Beal 2012 ). Sex steroid hormones, especially estrogens, have been widely investigated in relation to brain aging. Multiple lines
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Introduction Thyroid hormones (THs) are critical regulators of cellular differentiation, growth and metabolism. The thyroid secretes 100% of circulating thyroxine (T 4 ). However, it provides a low percentage of serum levels of the most
Local control of thyroid hormone action: role of type 2 deiodinase
Deiodinases: the balance of thyroid hormone
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Introduction Thyroid hormones are important homeostatic regulators that act via nuclear thyroid hormone receptors (TRs) in virtually all tissues during development and throughout postnatal life. 3,5,3′,5′- l -tetraiodothyronine (thyroxine, T 4 ) is
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The Medical Unit, The Princess Margaret Hospital, Christchurch 2, New Zealand
(Revised manuscript received 21 August 1978)
The frequent clinical and research requirement for measurement of both plasma luteinizing hormone (LH) and follicle-stimulating hormone (FSH) has prompted the development of a simultaneous radioimmunoassay for these two hormones. The considerable advantages of a simultaneous method include an economy of plasma volume, assay reagents, test-tubes and, more importantly, the time required for radioactive counting and performance of the assay by technical staff. The latter two factors comprise a significant proportion of radioimmunoassay operating costs.
This report describes a simultaneous radioimmunoassay based on the use of 131I-labelled FSH, 125I-labelled LH, anti-FSH serum M93 6873 (a generous gift from Professor W. R. Butt, Birmingham), anti-human chorionic gonadotrophin (HCG) serum for LH measurement (Donald, 1972) and donkey anti-rabbit precipitating serum (Wellcome Reagents, U.K.) for separation of antibody-bound and free hormones. Pituitary gonadotrophin standard (LER 907)
Max Planck Institute for Experimental Endocrinology, Department of Cell and Molecular Biology, Fritz-Lipmann-Institut, Institute of Biomedicine, Institute for Biochemistry, University of East Anglia, Feodor-Lynen-Str. 7, D-30625 Hannover, Germany
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Max Planck Institute for Experimental Endocrinology, Department of Cell and Molecular Biology, Fritz-Lipmann-Institut, Institute of Biomedicine, Institute for Biochemistry, University of East Anglia, Feodor-Lynen-Str. 7, D-30625 Hannover, Germany
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also in the pituitary of the athyroid Pax8 −/− mouse. These mice lack the thyroid hormone (TH) producing follicular cells of the thyroid gland and are thus completely athyroid in postnatal life ( Mansouri et al . 1998 ). These mutants can therefore be
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands
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in women, may indicate that sex hormones are implicated in its expression, etiology and course. The brain contains receptors ( Kruijver et al. 2001 , 2003 , Toran-Allerand 2004 ) for androgens and estrogens, and is capable of synthesizing and
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. 2004 ). These feedback signals may include a number of ascending neural inputs (e.g., signaling via the vagal nerve of gastric distension) and hormonal changes, including those of orexigenic or anorexigenic hormones released from gastrointestinal or
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ABSTRACT
Circulating free thyroid hormone concentrations are reduced in subjects taking long-term phenytoin, a finding at variance with their euthyroid clinical state and normal serum TSH concentration. It is suggested, therefore, that phenytoin may modify the cellular effects of thyroid hormones.
In order to examine the influence of phenytoin on thyroid hormone action in the pituitary gland we studied its effect on the binding of tri-iodothyronine (T3) to isolated nuclei prepared from rat anterior pituitary tissue. Phenytoin inhibited the nuclear binding of T3 in a dose-dependent fashion. Phenytoin also partially inhibited thyrotrophin-releasing hormone-stimulated TSH release from cultured rat anterior pituitary cells. These studies provide evidence for a direct effect of phenytoin on the thyrotroph mediated via nuclear T3 receptor binding.
J. Endocr. (1985) 104, 201–204
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SUMMARY
An immunoradiometric assay for parathyroid hormone has been developed. Antisera to bovine parathyroid hormone (BPTH) were screened for their ability to bind BPTH and human parathyroid hormone (HPTH). A BPTH-immunoadsorbent was used to extract antibodies from an antiserum which did not discriminate between BPTH and HPTH in a standard radioimmunoassay. These antibodies were labelled with 125I for use in the immunoradiometric assay. With this system as little as 5 pg BPTH and 8 pg HPTH could be detected. The serum concentration of BPTH was shown to rise in a cow rendered hypocalcaemic by an infusion of EDTA. Sera from patients with hyperparathyroidism contained high concentrations of hormone. These sera were found to dilute-out parallel to calibration curves obtained using HPTH extracted from parathyroid adenomata. The advantages of this method over the standard radioimmunoassay are discussed.
The University of Adelaide, Discipline of Medicine, Adelaide Medical School, Adelaide, Australia
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Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
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Health Sciences, UCLA, Los Angeles, California, USA
The Lundquist Institute, Harbor-UCLA Medical Center, Torrance, California, USA
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Introduction Regulation of many physiological parameters involves feedback loops that include a controlling hormone and a target parameter. In endocrinology, the target parameter itself may be a hormone. The stimulatory and inhibitory
