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K. E. BEERY
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J. D. SINK
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3α-Hydroxy-5α-androst-16-ene and 5α-androst-16-en-3-one were isolated from the subcutaneous adipose tissue of swine and identified (Fig. la, b). These steroids, when excreted or secreted, could function as sex pheromones and initiate the olfactory communication stimulus regulating the mating behaviour of the domestic pig (Sus domesticus) (Signoret & du Buisson, 1961). Other researchers (Lerche, 1936; Craig, Pearson & Webb, 1962) have already established that a particularly strong sex odour (variously described as musk-, perspiration- and urine-like) was associated with the fatty tissues of the animal carcass. Even though the odoriferous, musksmelling androstenols have been isolated from the testis (Prelog & Ruzicka, 1944), adrenal (Gower & Ahmad, 1966) and salivary glands (Patterson, 1968b) of the pig, they have not been identified as components of fatty or adipose tissue. Stimulated by our theoretical prediction (Sink, 1967) that 3α-hydroxy-5α-androst-16-ene should be present, and Patterson's subsequent finding (Patterson, 1968a) of the oxidized form,

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Chatsri Deachapunya
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Sutthasinee Poonyachoti Department of Physiology, Department of Physiology, Consortium for Calcium and Bone Research, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand

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Nateetip Krishnamra Department of Physiology, Department of Physiology, Consortium for Calcium and Bone Research, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand
Department of Physiology, Department of Physiology, Consortium for Calcium and Bone Research, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand

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epithelial cells ( Matthews et al . 1993 ) and the intact porcine endometrial epithelium ( Vetter & O'Grady 1996 ) have provided direct evidence for the regulation of Na + absorption and K + secretion. In the primary culture of mouse and porcine

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Hiroki Otsubo Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Susumu Hyodo Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Hirofumi Hashimoto Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Makoto Kawasaki Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Hitoshi Suzuki Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Takeshi Saito Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Toyoaki Ohbuchi Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Toru Yokoyama Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Hiroaki Fujihara Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Tetsuro Matsumoto Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Yoshio Takei Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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Yoichi Ueta Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

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cannula at a depth of 1.0 mm beyond the end of the guide. The total volume of injected solution of AM5 and vehicle into the lateral ventricle was 5 μl. Porcine AM5 was kindly provided by Peptide Institute (Minoh, Japan). Rat AM, human CGRP-(8–37), and

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Tetsuya Ito Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro 080-8555, Japan

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Hnin ThidarMyint Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro 080-8555, Japan

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Takahiro Murata Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro 080-8555, Japan

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Hiroaki Inoue Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro 080-8555, Japan

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Roel Mata Neyra Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro 080-8555, Japan

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Hideto Kuwayama Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro 080-8555, Japan

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Introduction Peptide YY (PYY), a 36-amino-acid peptide that shares a common tertiary structure with neuropeptide Y (NPY) and pancreatic polypeptide (PP) ( Berglund et al. 2003 ), was initially isolated from porcine intestine

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A Mostyn
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J C Litten
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K S Perkins
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M C Alves-Guerra
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C Pecqueur
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B Miroux
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M E Symonds
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L Clarke
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porcine temperature) within 48 h after birth, indicating that homeothermy is progressively achieved ( Mount 1968 ). In order to adapt to this large change in environmental temperature, most mammalian species possess brown adipose tissue ( Nedergaard

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P. B. GREENBERG
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T. J. MARTIN
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R. A. MELICK
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P. JABLONSKI
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J. McK. WATTS
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SUMMARY

The metabolic clearance rate (MCR) of porcine calcitonin in six gilts was 13·0 ± 0·7 (s.e.m.) ml/min/kg and in four isolated, perfused pig livers was 16·1 ± 1·1 ml/min, with constant infusions of porcine calcitonin and measurement of calcitonin concentration by radioimmunoassay.

After single injections of 125I-labelled porcine calcitonin, the MCR of 125I-labelled porcine calcitonin was 29·2 ± 2·8 ml/min in six isolated, perfused pig livers.

125I-labelled salmon calcitonin was cleared at a slower rate than porcine calcitonin after single injections into the gilt. 125I-Labelled human calcitonin and 125I-labelled salmon calcitonin were not significantly cleared by the isolated, perfused pig liver.

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Agnieszka Waclawik Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

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Adam J Ziecik Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

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downstream enzymes: PGE synthase (mPGES-1), PGF synthase (PGFS), and PG 9-ketoreductase in conceptus development. Although changes of PG concentration in porcine uterine lumen are well characterized in the estrous cycle and early pregnancy, content of PGs was

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Itaru Minagawa Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Dai Sagata Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Ali Mohammed Pitia Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Hiroshi Kohriki Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Masatoshi Shibata Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Hiroshi Sasada Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Yoshihisa Hasegawa Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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Tetsuya Kohsaka Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
Laboratory of Animal Reproduction and Physiology, Division of Animal Resource Production, Shizuoka Swine and Poultry Experimental Station, Laboratory of Animal Reproduction, School of Veterinary Medicine, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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specificity of the TR-FIA, inhibition curves for boar serum and testicular extracts were compared with the curve for the boar INSL3 standard. Cross-reactivity with the 6-kDa porcine RLN purified from pregnant sow ovaries ( Kohsaka et al . 1993 ), insulin

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N Govoni Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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R De Iasio Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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C Cocco Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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A Parmeggiani Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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G Galeati Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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U Pagotto Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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C Brancia Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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M Spinaci Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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C Tamanini Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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R Pasquali Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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G-L Ferri Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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E Seren Department of Veterinary Morphophysiology and Animal Production, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Endocrinology Unit and Center for Applied Biomedical Research (CRBA), Sant’ Orsola-Malpighi Hospital, 40100 Bologna (BO), Italy
NEF-Laboratory, Department of Cytomorphology, University of Cagliari at Monserrato, 09042 Monserrato (CA), Italy

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prepubertal gilts were to study both distribution and possible changes of acyl-ghrelin-immunoreactive cells in the main areas of gastric mucosa, to validate an RIA for measurement of acyl-ghrelin in porcine plasma and to determine plasma acyl-ghrelin profiles

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N Hattori Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi-City, Osaka 570-8506, Japan
Department of Endocrinology, Kobe City General Hospital, Kobe 650-0046, Japan

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Y Nakayama Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi-City, Osaka 570-8506, Japan
Department of Endocrinology, Kobe City General Hospital, Kobe 650-0046, Japan

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K Kitagawa Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi-City, Osaka 570-8506, Japan
Department of Endocrinology, Kobe City General Hospital, Kobe 650-0046, Japan

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T Ishihara Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi-City, Osaka 570-8506, Japan
Department of Endocrinology, Kobe City General Hospital, Kobe 650-0046, Japan

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Y Saiki Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi-City, Osaka 570-8506, Japan
Department of Endocrinology, Kobe City General Hospital, Kobe 650-0046, Japan

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C Inagaki Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi-City, Osaka 570-8506, Japan
Department of Endocrinology, Kobe City General Hospital, Kobe 650-0046, Japan

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unknown in 15 of the 18 macroprolactinemic patients (idiopathic hyperprolactinemia) and they almost entirely lacked the clinical symptoms of hyperprolactinemia, such as amenorrhea and galactorrhea. Human, porcine, bovine, and rat PRL and human growth

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