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Ann E Drummond Prince Henry's Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, Australia

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Peter J Fuller Prince Henry's Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, Australia

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identify genes regulated by ERβ in normal tissues have yet to be published; the few undertaken to date have utilised cancer lines (see Zhao et al . (2008) for a summary). Chang et al . (2006) investigated the effect of ERβ on gene regulation by MCF-7

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Georgia Papacleovoulou The Queen's Medical Research Institute, Centre for Reproductive Biology, Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh EH16 4TJ, UK

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Hilary O D Critchley The Queen's Medical Research Institute, Centre for Reproductive Biology, Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh EH16 4TJ, UK

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Stephen G Hillier The Queen's Medical Research Institute, Centre for Reproductive Biology, Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh EH16 4TJ, UK

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J Ian Mason The Queen's Medical Research Institute, Centre for Reproductive Biology, Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh EH16 4TJ, UK

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represent significance between different treatments. Results Effects of IL4 on steroid-related gene regulation in IL1α-treated hOSE cells Effects of pro-inflammatory IL1α and anti-inflammatory IL4 on 3β-HSD1 , 3β-HSD2 , AR and PR transcriptional

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Ellen H Stolte Department of Animal Physiology, Radboud University, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG, Wageningen, The Netherlands

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B M Lidy Verburg van Kemenade Department of Animal Physiology, Radboud University, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG, Wageningen, The Netherlands

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Huub F J Savelkoul Department of Animal Physiology, Radboud University, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG, Wageningen, The Netherlands

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Gert Flik Department of Animal Physiology, Radboud University, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG, Wageningen, The Netherlands

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corticoresistant guinea pig. Endocrinology 104 344 –349. Kumar R & Thompson EB 1999 The structure of the nuclear hormone receptors. Steroids 64 310 –319. Kumar R & Thompson EB 2005 Gene

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Nilli Zmora Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
Research Group Endocrinology and Metabolism, Department Biology, Faculty of Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Physiology of Growth and Reproduction in Fish, Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway

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Yukinori Kazeto Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
Research Group Endocrinology and Metabolism, Department Biology, Faculty of Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Physiology of Growth and Reproduction in Fish, Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway

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R Sampath Kumar Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
Research Group Endocrinology and Metabolism, Department Biology, Faculty of Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Physiology of Growth and Reproduction in Fish, Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway

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Rüdiger W Schulz Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
Research Group Endocrinology and Metabolism, Department Biology, Faculty of Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Physiology of Growth and Reproduction in Fish, Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway

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John M Trant Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
Research Group Endocrinology and Metabolism, Department Biology, Faculty of Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Physiology of Growth and Reproduction in Fish, Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway

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the rec -FSH and rec -LH preparations on steroidogenesis in vitellogenic Ip follicles was determined by the changes in E 2 secretion and P450arom gene regulation (transcript abundance). As seen in Fig. 5A , both LH and FSH induced E 2 secretion

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Kyuyong Han Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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Haengseok Song Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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Irene Moon Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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Robert Augustin Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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Kelle Moley Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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Melissa Rogers Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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Hyunjung Lim Department of Obstetrics and Gynecology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA

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gene regulation studies, male mice received an i.p. injection of 5 μM 9- cis -retinoic acid (9-cRA) and/or cPGI and were killed at several time points for RNA collection. Vehicle (5% dimethyl sulfoxide (DMSO) + 95% PBS)-injected mice served as controls

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Evelyn Davies Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK

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Selma Omer Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK

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John F Morris Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK

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Helen C Christian Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK

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rats. Journal of Cellular Biochemistry 77 560 –568. Mitchner NA , Garlick C & Ben-Jonathan N 1998 Cellular distribution and gene regulation of estrogen receptors α and β in the rat pituitary gland. Endocrinology 139

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Dominique A Glauser Fondation pour Recherches Médicales, University of Geneva, Av. de la Roseraie 64, 1211 Geneva, Switzerland

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Werner Schlegel Fondation pour Recherches Médicales, University of Geneva, Av. de la Roseraie 64, 1211 Geneva, Switzerland

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. 2003 Insulin-regulated hepatic gluconeogenesis through FOXO1-PGC-1alpha interaction. Nature 423 550 –555. Ramaswamy S , Nakamura N, Sansal I, Bergeron L & Sellers WRA 2002 A novel mechanism of gene

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Itsuo Murakami Department of Biology, Faculty of Science, Okayama University, 3-1-1, Tsusima-naka, Okayama 700-8530, Japan
Department of Biological Pharmacy, School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan

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Sakae Takeuchi Department of Biology, Faculty of Science, Okayama University, 3-1-1, Tsusima-naka, Okayama 700-8530, Japan
Department of Biological Pharmacy, School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan

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Toshiyuki Kudo Department of Biology, Faculty of Science, Okayama University, 3-1-1, Tsusima-naka, Okayama 700-8530, Japan
Department of Biological Pharmacy, School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan

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Shizuyo Sutou Department of Biology, Faculty of Science, Okayama University, 3-1-1, Tsusima-naka, Okayama 700-8530, Japan
Department of Biological Pharmacy, School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan

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Sumio Takahashi Department of Biology, Faculty of Science, Okayama University, 3-1-1, Tsusima-naka, Okayama 700-8530, Japan
Department of Biological Pharmacy, School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan

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induction of NUR77/NURR1 in corticotrophsby CRH/cAMP: involvement of calcium, protein kinase A, and MAPK pathways. Molecular Endocrinology 16 1638 –1651. Kumar R & Thompson EB 2005 Gene regulation by the

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José C Garrido-Gracia Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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Ana Gordon Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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Carmina Bellido Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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Rafaela Aguilar Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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Inmaculada Barranco Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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Yolanda Millán Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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Juana Martín de las Mulas Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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José E Sánchez-Criado Departments of Cell Biology, Physiology and Immunology and
Comparative Pathology, University of Córdoba, Córdoba, Spain

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gene regulation of estrogen receptors α and β in the rat pituitary gland. Endocrinology 139 3976 –3983. Neef G , Beier S, Elger W, Henderson D & Wiechert R 1984 New steroid with antiprogestational and

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Yan Zhou Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

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Jacob Bendor Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

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Lauren Hofmann Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

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Matthew Randesi Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

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Ann Ho Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

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Mary Jeanne Kreek Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

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between these two parallel increases. Therefore, this suggests that the regulation by acute morphine withdrawal of lat.hyp orexin gene expression is not directly related to either the MOP-r or ppDyn gene regulation in the same region. MOP

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