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Hao Wu Department of Nephrology, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China
The ‘973’ National Basic Research Program of China, Changchun University of Chinese Medicine, Changchun, Jilin, People’s Republic of China

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Junduo Wu Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China

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Shengzhu Zhou Department of Anesthesiology, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China

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Wenlin Huang School of Science and Technology, Georgia Gwinnett College, Lawrenceville, Georgia, USA

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Ying Li Department of Dermatology, Affiliated Hospital of Beihua University, Jilin, Jilin, People’s Republic of China

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Huan Zhang Operating Theatre, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China

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Junnan Wang Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China

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Ye Jia Department of Nephrology, The First Hospital of Jilin University, Changchun, Jilin, People’s Republic of China

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Biotechnology; 1:100), SIRT1 (Abcam; 1:100) and acetylated P53 (Ac-P53, Abcam; 1:100). IHC-positive area was quantified within the full-thickness of the artery wall, including endothelium, tunica media and tunica externa. Selection of areas to photograph and

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Amanda K Mauro Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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Nauman Khurshid Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA
Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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Danielle M Berdahl Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA
Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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Amanda C Ampey Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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Daniel Adu Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA
Department of Pediatrics, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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Dinesh M Shah Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA
Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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Derek S Boeldt Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin – Madison, School Medicine and Public Health, Madison, Wisconsin, USA

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. While the reason for this is unclear, it is reasonable that the endothelium would have reserve capacity to acutely increase vasodilation and blood flow in response to minor insults (e.g. transient hypoxia), while quickly reducing vasodilator output and

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Charlotte Lawson Department of Comparative Biomedical Sciences, The Hatter Cardiovascular Institute, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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Jose M Vicencio Department of Comparative Biomedical Sciences, The Hatter Cardiovascular Institute, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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Derek M Yellon Department of Comparative Biomedical Sciences, The Hatter Cardiovascular Institute, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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Sean M Davidson Department of Comparative Biomedical Sciences, The Hatter Cardiovascular Institute, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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excess androgen activity, oligo-ovulation or anovulation, and polycystic ovaries ( Teede et al . 2010 ). The role EVs in the function and dysfunction of healthy and diseased endothelium A number of studies have demonstrated a correlation between the

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D S Boeldt Perinatal Research Laboratories, Department of Obstetrics and Gynecology, University of Wisconsin‐Madison, 7E Meriter Hospital/Park, 202 South Park Street, Madison, Wisconsin 53715, USA

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F X Yi Perinatal Research Laboratories, Department of Obstetrics and Gynecology, University of Wisconsin‐Madison, 7E Meriter Hospital/Park, 202 South Park Street, Madison, Wisconsin 53715, USA

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I M Bird Perinatal Research Laboratories, Department of Obstetrics and Gynecology, University of Wisconsin‐Madison, 7E Meriter Hospital/Park, 202 South Park Street, Madison, Wisconsin 53715, USA

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endothelium as a byproduct of the conversion of l -arginine, NADPH, and oxygen to l -citrulline, NADP + , and H + by the enzyme endothelial NO synthase (eNOS). Once formed, NO acutely acts on underlying vascular smooth muscle cells to reduce tension and so

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Hélène Buteau-Lozano INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France
INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France

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Guillaume Velasco INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France
INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France

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Monique Cristofari INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France
INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France

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Patrick Balaguer INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France

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Martine Perrot-Applanat INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France
INSERM, IFR105 Université Paris 7, INSERM, U553, Hémostase, Endothélium et Angiogénèse, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France

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Environmental chemicals may affect human health by disrupting endocrine function. Their possible role in the mammary gland and breast tumors is still unknown. Previous studies have demonstrated that vascular endothelial growth factor (VEGF), a key factor in angiogenesis and tumor progression, is an estrogen-regulated gene. We analyzed whether VEGF expression is regulated by different xenoestrogens in several breast cancer cells, MELN (derived from MCF-7) and MELP (derived from MDA-MB-231) and stably expressing estrogen receptor α (ERα); these cell lines stably express estrogen response element (β-globin)-luciferase. Genistein, bisphenol A (BPA), 4-(tert-octyl)phenol (OP), dieldrin, and several phthalates, including benzyl butyl phthalate (BBP) and di-ethyl-2-hexyle phthalate (DEHP), were first shown to be estrogenic. These compounds induced a dose-dependent increase of VEGF secretion in MELN and MCF-7 cells; maximal effect was observed at 1–10 μM non-cytotoxic concentrations and was inhibited by the antiestrogen ICI 182 780. VEGF increase was not observed in ERα-negative MDA-MB-231 cells. Most substances increased VEGF transcript levels in MELN cells. In contrast, γ-hexachlorocyclohexane, vinclozolin, and the phthalates (mono-n-butyl ester phthalic acid, di-isononyle phthalate, and di-isodecyle phthalate) were ineffective on both VEGF secretion and estrogenic luciferase induction in these cell lines. Specific kinase inhibitors PD98059, SB203580, or LY294002 suppressed the xenoestrogen-induced VEGF response, suggesting activation of MEK, p38 kinase, and phosphatidylinositol-3-kinase pathways. Our in vitro results show for the first time that genistein and xenoestrogens (BPA, OP, dieldrin, BBP, and DEHP at high concentrations) up-regulate VEGF expression in MELN cells by an ER-dependent mechanism. Since VEGF increases capillary permeability and breast tumor angiogenesis in vivo, the physiological relevance of these findings is discussed.

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Rob H P Hilgers
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Suzanne Oparil
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Wout Wouters Division of Cardiovascular Disease, Pantarhei Bioscience, Department of Medicine, Vascular Biology and Hypertension Program, University of Alabama at Birmingham, 1034 Zeigler Research Building, 703 19th Street South, Birmingham, Alabama 35294-0007, USA

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Herjan J T Coelingh Bennink Division of Cardiovascular Disease, Pantarhei Bioscience, Department of Medicine, Vascular Biology and Hypertension Program, University of Alabama at Birmingham, 1034 Zeigler Research Building, 703 19th Street South, Birmingham, Alabama 35294-0007, USA

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aortic, carotid, mesenteric, pulmonary, renal, middle cerebral, and septal coronary arterial segments; 2) to assess the involvement of ERs in the vasorelaxing response to E 4 in uterine arteries; and 3) to assess the endothelium-dependent and endothelium

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Tsuneo Kobayashi Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan

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Takayuki Matsumoto Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan

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Katsuo Kamata Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan

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or after incubation (as required by that particular experiment), the endothelium was removed by rubbing the intimal surface with a cotton swab. Successful denudation was confirmed by the absence of a relaxation to 10 −5 M acetylcholine. Strips were

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R M McAllister Departments of Anatomy and Physiology and Kinesiology, Kansas State University, Manhattan, Kansas 66506, USA
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211, USA

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I Albarracin Departments of Anatomy and Physiology and Kinesiology, Kansas State University, Manhattan, Kansas 66506, USA
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211, USA

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E M Price Departments of Anatomy and Physiology and Kinesiology, Kansas State University, Manhattan, Kansas 66506, USA
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211, USA

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T K Smith Departments of Anatomy and Physiology and Kinesiology, Kansas State University, Manhattan, Kansas 66506, USA
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211, USA

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J R Turk Departments of Anatomy and Physiology and Kinesiology, Kansas State University, Manhattan, Kansas 66506, USA
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211, USA

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K D Wyatt Departments of Anatomy and Physiology and Kinesiology, Kansas State University, Manhattan, Kansas 66506, USA
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211, USA

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vasomotor responses were determined for euthyroid and hypothyroid rats, endothelium-dependent dilation was also found to be blunted in isolated aortae ( Delp et al. 1995 , McAllister et al. 2000 ) and isolated perfused kidneys ( Vargas et al. 1995

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D S Boeldt Department of Ob/Gyn, Perinatal Research Laboratories, University Wisconsin – Madison, Madison, Wisconsin, USA

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I M Bird Department of Ob/Gyn, Perinatal Research Laboratories, University Wisconsin – Madison, Madison, Wisconsin, USA

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to the organ/organ system level. A growing body of evidence points to the disruption of these incompletely understood processes as potential mediators of the symptoms of PE. More specifically, although many studies point to the vascular endothelium as

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Åsa Tivesten
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Anna Barlind
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Kenneth Caidahl
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Natalia Klintland
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Antonio Cittadini
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Claes Ohlsson
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Jörgen Isgaard
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. 2000 ), arterial stiffness ( Smith et al. 2002 ), endothelial and/or endothelium-independent vasodilation ( Rossoni et al. 1999 , Evans et al. 2000 , Capaldo et al. 2001 ), and may reverse markers of early atherosclerosis ( Pfeifer et al

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