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presence of a functional progesterone receptor, we performed the ligand blot assay. When the specificity of interaction was evaluated by binding competitions with unlabeled progesterone, progesterone antagonist RU486 or estrogen, unlabeled progesterone and
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, except the primary antibody was replaced with normal mouse serum. All results were expressed as the percentage of mitotic or labelled cells. Estrogen receptor-α, progesterone receptors and β-catenin Estrogen receptor
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). Figure 5 Effect of body weight (BW) on expression of estrogen-responsive genes IGF-I and progesterone receptor ( PR ) within the mammary parenchyma (PAR) and mammary fat pad (MFP). Dairy heifers were offered a restricted or an elevated plane of
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progesterone in serum from young and old rats, and found that estrogen levels were higher in the 18 months old rat than in the 3 months old rat, and higher again in rats of 22.5, 25.5, and 26 months old. This agrees with the observations of Chakraborty & Gore
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baseline mRNA expression levels of estrogen-metabolizing enzymes in spheroid-cultured stromal cells (SCs) derived from normal endometrium (NE), endometrium with endometriosis (EE), and OE. (B) Effects of dienogest (DNG) and progesterone (P 4 ) on mRNA
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(100 ng/mouse) or progesterone (2 mg/mouse) ( Guo et al . 2012 ). The uteri were then collected 1, 3, 6, 12, and 24 h after steroid treatment. To examine whether nuclear receptors for estrogen or progesterone are involved in steroid hormonal regulation
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Introduction The single transmembrane protein progesterone receptor membrane component 1 (PGRMC1) is a 25–28 kDa protein that possesses a cytochrome b -like heme-binding domain and belongs to the membrane-associated progesterone receptor (MAPR
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limbic–preoptic–hypothalamic regions ( Goodman & Knobil 1981 ). The LH surge induced by estrogen is stimulated and thereafter inhibited when progesterone, another ovarian hormone, acts after estrogen priming ( Brann & Mahesh 1991 ). Secondly, the GnRH
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. Priming of the uterus to become receptive is initiated by ovarian-derived estrogen and progesterone and requires intricately coordinated signaling between the luminal and glandular epithelia (LE and GE, respectively) and the stroma ( Wang & Dey 2006
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typical estrogenic effect, as IGF-I causes estrogen-induced proliferation of uterine tissue and prepares the endometrium for possible pregnancy ( Fazleabas et al. 2004 ), and this response is specified by the high progesterone levels after ovulation
