Search Results
Search for other papers by J-W Jeong in
Google Scholar
PubMed
Search for other papers by K Y Lee in
Google Scholar
PubMed
Search for other papers by J P Lydon in
Google Scholar
PubMed
Search for other papers by F J DeMayo in
Google Scholar
PubMed
Introduction The uterus consists of heterogeneous cell types that undergo dynamic changes to support embryo development and implantation. These phenomena are primarily dependent on coordinated interactions mediated by progesterone (P
Search for other papers by Pei-Jian He in
Google Scholar
PubMed
Search for other papers by Masami Hirata in
Google Scholar
PubMed
Search for other papers by Nobuhiko Yamauchi in
Google Scholar
PubMed
Search for other papers by Masa-aki Hattori in
Google Scholar
PubMed
uterus ( Dolatshad et al. 2006 ). At the molecular level, the clock system is composed of interlocked transcriptional and translational feedback loops. The CLOCK and brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein 1 (BMAL1
Search for other papers by Andrei G Gunin in
Google Scholar
PubMed
Search for other papers by Irina N Kapitova in
Google Scholar
PubMed
Search for other papers by Nina V Suslonova in
Google Scholar
PubMed
Introduction Estrogen hormones have a variety of effects in the uterus. Proliferation and changes in structure and architecture of uterine tissues are estrogen-dependent events ( Martin et al. 1973 , Bigsby 2002 ). Endometrial
Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Faculty of Biology, Institute of Zoology, Technische Universität Dresden, Dresden, Germany
Search for other papers by María Victoria Zanardi in
Google Scholar
PubMed
Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Search for other papers by María Paula Gastiazoro in
Google Scholar
PubMed
Departamento de Bioquímica Clínica y Cuantitativa, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Search for other papers by María Florencia Rossetti in
Google Scholar
PubMed
Search for other papers by Florencia Doná in
Google Scholar
PubMed
Departamento de Bioquímica Clínica y Cuantitativa, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Search for other papers by Gisela Paola Lazzarino in
Google Scholar
PubMed
Search for other papers by Oliver Zierau in
Google Scholar
PubMed
Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Search for other papers by Jorgelina Varayoud in
Google Scholar
PubMed
Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Search for other papers by Milena Durando in
Google Scholar
PubMed
postnatal day (PND)21 up to PND140 ( Gastiazoro et al. 2018 ). In the present study, our hypothesis was that GBH exacerbates the effects of CAF diet on the rat uterus. Therefore, we proposed to evaluate the effects of the addition of a subchronic low dose
Search for other papers by J Varayoud in
Google Scholar
PubMed
Search for other papers by J G Ramos in
Google Scholar
PubMed
Search for other papers by L Monje in
Google Scholar
PubMed
Search for other papers by V Bosquiazzo in
Google Scholar
PubMed
Search for other papers by M Muñoz-de-Toro in
Google Scholar
PubMed
Search for other papers by E H Luque in
Google Scholar
PubMed
activating protein-1 (AP-1) sites, depending upon the structural properties of the ligand ( Paech et al. 1997 ). The rat uterus, a major target tissue for ovarian steroids, has served as an excellent model for studying hormonal regulation of ERα
Medical Microbiology and
Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates
Department of Obstetrics and Gynecology, University Hospital, SE 221 85, Lund, Sweden
Zoonoses and Emerging Infections Group, Clinical Virology, University of Veterinary Medicine, Clinical Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria
Search for other papers by T Bossmar in
Google Scholar
PubMed
Medical Microbiology and
Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates
Department of Obstetrics and Gynecology, University Hospital, SE 221 85, Lund, Sweden
Zoonoses and Emerging Infections Group, Clinical Virology, University of Veterinary Medicine, Clinical Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria
Search for other papers by N Osman in
Google Scholar
PubMed
Medical Microbiology and
Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates
Department of Obstetrics and Gynecology, University Hospital, SE 221 85, Lund, Sweden
Zoonoses and Emerging Infections Group, Clinical Virology, University of Veterinary Medicine, Clinical Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria
Search for other papers by E Zilahi in
Google Scholar
PubMed
Medical Microbiology and
Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates
Department of Obstetrics and Gynecology, University Hospital, SE 221 85, Lund, Sweden
Zoonoses and Emerging Infections Group, Clinical Virology, University of Veterinary Medicine, Clinical Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria
Search for other papers by M A El Haj in
Google Scholar
PubMed
Medical Microbiology and
Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates
Department of Obstetrics and Gynecology, University Hospital, SE 221 85, Lund, Sweden
Zoonoses and Emerging Infections Group, Clinical Virology, University of Veterinary Medicine, Clinical Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria
Search for other papers by N Nowotny in
Google Scholar
PubMed
Medical Microbiology and
Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates
Department of Obstetrics and Gynecology, University Hospital, SE 221 85, Lund, Sweden
Zoonoses and Emerging Infections Group, Clinical Virology, University of Veterinary Medicine, Clinical Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria
Search for other papers by J M Conlon in
Google Scholar
PubMed
-like immunoreactivity, in rat uterus and human amnion, chorion and decidua ( Lundin et al. 1989 , Lefebvre et al. 1992 , Chibbar et al. 1993 ). These findings indicate a uterine origin and synthesis of OT. A possible paracrine action of this hormone may
Search for other papers by M Maggi in
Google Scholar
PubMed
Search for other papers by G B Vannelli in
Google Scholar
PubMed
Search for other papers by G Fantoni in
Google Scholar
PubMed
Search for other papers by E Baldi in
Google Scholar
PubMed
Search for other papers by A Magini in
Google Scholar
PubMed
Search for other papers by A Peri in
Google Scholar
PubMed
Search for other papers by S Giannini in
Google Scholar
PubMed
Search for other papers by L Gloria in
Google Scholar
PubMed
Search for other papers by P Del Carlo in
Google Scholar
PubMed
Search for other papers by D Casparis in
Google Scholar
PubMed
Search for other papers by T Tomei in
Google Scholar
PubMed
Search for other papers by M Serio in
Google Scholar
PubMed
Abstract
In this study we report the immunolocalization, binding and biological activity of endothelins in the human uterus. Since, in previous studies in the rabbit, sex steroids greatly affected uterine endothelin-1 (ET-1) immunolocalization and binding, we sought to compare results obtained in a relatively steroid-deprived uterus (postmenopausal women) with those obtained in late pregnancy. Two classes of ET receptors were identified in human pregnant and non-pregnant myometrium. One site (ETB) was a low capacity site (0·3 pm/mg protein) that bound with high affinity (0·1 nm), yet no selectivity, ET-1, ET-2, ET-3, sarafotoxin (SRTX) and vasoactive intestinal contractor (VIC). The second site (ETA) was six fold more concentrated than the former (1·9 pm/mg protein) and was relatively selective for ET-1, ET-2 and VIC but showed lower affinity for ET-3 and SRTX. Studies with human myometrial cells indicated that the ETA receptor mediates an increase in intracellular calcium, while the physiological function of the ETB receptor is still unclear. Homologous competition curves for ET-1 were used in order to study the ET receptor density (ETA+ETB) in individual myometrial samples. We found that the concentration of ET receptors did not change during different stages of labour or in postmenopausal women. We identified cells with intense positivity for ET-1 in human decidua. Similar cells were also present in pregnant myometrium, intimately associated with smooth muscle cells. Conversely, no staining for ET-1 was observed in non-pregnant myometrium. A paracrine role for ET-1 in the human uterus is suggested.
Journal of Endocrinology (1994) 142, 385–396
Search for other papers by W. J. BO in
Google Scholar
PubMed
Search for other papers by L. E. MARASPIN in
Google Scholar
PubMed
Search for other papers by MAVIS S. SMITH in
Google Scholar
PubMed
SUMMARY
Glycogen synthetase, phosphorylase and glycogen were determined biochemically in the smooth muscle of the rat uterus following a single s.c. injection (10 μg.) of oestradiol dipropionate. The ovariectomized animals were killed 6, 12, 24, 48, 72 and 96 hr. after the hormone treatment. From 12 to 96 hr. glycogen synthetase activity was significantly greater than in the untreated control rats. Phosphorylase activity was significantly less than in the controls at 12 hr. and greater from 48 to 96 hr. After the initial drop, control phosphorylase values were obtained between 24 and 48 hr. From 12 to 96 hr. the glycogen concentration was greater than in the control animals.
The results show that oestrogen increased glycogen synthetase activity in the smooth muscle of the uterus soon after the hormone treatment, and with the increase in enzymic activity the glycogen concentration was also increased. They indicate that, during the early phase of glycogen synthesis, oestrogen stimulates glycogenesis by increasing glycogen synthetase activity and suppresses glycogenolysis by inhibiting phosphorylase activity. The glycogen concentration at later stages did not alter significantly, and this may have been due to the build-up and breakdown of the carbohydrate by the action of glycogen synthetase and phosphorylase, respectively.
Search for other papers by C D Simmons in
Google Scholar
PubMed
Search for other papers by J M P Pabona in
Google Scholar
PubMed
Search for other papers by Z Zeng in
Google Scholar
PubMed
Search for other papers by M C Velarde in
Google Scholar
PubMed
Search for other papers by D Gaddy in
Google Scholar
PubMed
Search for other papers by F A Simmen in
Google Scholar
PubMed
Search for other papers by R C M Simmen in
Google Scholar
PubMed
-regulators ( Heldring et al . 2007 ). In the uterus, ESR1 is the predominant isoform, and its presence is necessary for reproductive functions ( Couse & Korach 1999 , Matthews & Gustafsson 2003 ). Indeed, mice null for Esr1 are infertile in contrast to Esr2 KO
Search for other papers by J. M. ROBSON in
Google Scholar
PubMed
Search for other papers by J. R. TROUNCE in
Google Scholar
PubMed
Search for other papers by K. A. H. DIDCOCK in
Google Scholar
PubMed
SUMMARY
1. The sensitivity of the rat uterus to serotonine (5-hydroxy-tryptamine) is high in oestrus, but appears to diminish in dioestrus.
2. The sensitivity of the rat uterus to serotonine is high in pregnancy, but diminishes in the immediate post-partum period.
3. Ovariectomy diminishes the sensitivity of the rat uterus to serotonine. Sensitivity can be restored by oestradiol and stilboestrol, but not by some other steroid hormones.
4. Some blocking agents so far described do not appear to be specific for serotonine.
5. An inhibiting effect of parachlorometacresol on the spontaneous activity and response of the uterus to serotonine is described.
