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Sarah L Armour Section for Cell Biology and Physiology, Department of Biology, University of Copenhagen, Denmark

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Jade E Stanley Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

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James Cantley Division of Cellular and Systems Medicine, School of Medicine, University of Dundee, UK

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E Danielle Dean Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
Division of Diabetes, Endocrinology, & Metabolism, Vanderbilt University Medical Center School of Medicine, Nashville, Tennessee, USA

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Jakob G Knudsen Section for Cell Biology and Physiology, Department of Biology, University of Copenhagen, Denmark

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-1298 ) Rakhshandehroo M Hooiveld G Müller M & Kersten S 2009 Comparative analysis of gene regulation by the transcription factor PPARα between mouse and human . PLoS One 4 e6796 . ( https://doi.org/10.1371/journal.pone.0006796 ) Rocha DM Faloona GR

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Marianna Minnetti Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy

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Valeria Hasenmajer Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy

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Riccardo Pofi Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy

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Mary Anna Venneri Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy

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Krystallenia I Alexandraki Endocrine Unit, 1st Department of Propaedeutic Medicine, Laiko University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece

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Andrea M Isidori Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy

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Garabedian MJ Myers RM 2009 Genomic determination of the glucocorticoid response reveals unexpected mechanisms of gene regulation . Genome Research 19 2163 – 2171 . ( https://doi.org/10.1101/gr.097022.109 ) Reddy TE Gertz J Crawford GE

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Femke L Groeneweg
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Henk Karst Department of Medical Pharmacology, Department of Neuroscience and Pharmacology, Leiden Amsterdam Center for Drug Research and Leiden University Medical Center, Leiden University, Einsteinweg 55, 2333CC Leiden, The Netherlands

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E Ron de Kloet
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Marian Joëls Department of Medical Pharmacology, Department of Neuroscience and Pharmacology, Leiden Amsterdam Center for Drug Research and Leiden University Medical Center, Leiden University, Einsteinweg 55, 2333CC Leiden, The Netherlands

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-Pacheco 1996 ). Activation of the receptors results in waves of gene regulation, with both transactivation and transrepression of responsive genes ( Datson et al . 2008 ). Although the MR and GR share almost identical DNA-binding domains, they do not bind to

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Jinke Wang The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China

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Jie Lu The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China

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Guangming Gu The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China

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Yingxun Liu The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China

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). Determination of the relative binding affinity of different TFs to their various DNA-binding sites is fundamentally important for a comprehensive understanding of gene regulation. The whole profile of DNA-binding affinity of a TF protein to all possible DNA

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Cátia F Gonçalves Wellcome Centre for Cell Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine & Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK

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Qing-Jun Meng Wellcome Centre for Cell Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine & Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK

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JR Jager J Soccio RE , et al . 2015 GENE REGULATION: Discrete functions of nuclear receptor Rev-erbα couple metabolism to the clock . Science 1488 – 1492 . ( https://doi.org/10.1126/science.aab3021 ) 26044300 Zvonic S Ptitsyn AA

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Ivana Vaněčková Institute of Physiology, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic

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Lenka Maletínská Institute of Physiology, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic

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Michal Behuliak Institute of Physiology, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic

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Veronika Nagelová Institute of Physiology, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic

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Josef Zicha Institute of Physiology, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic

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Jaroslav Kuneš Institute of Physiology, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic

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that are notoriously difficult to identify. Thus, epigenetic changes and regulation become increasingly important. The research community is increasingly aware of the role that chromatin plays in gene regulation, transcription, and disease development

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H El Sheikh Saad
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A Toullec
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S Vacher INSERM U965, Laboratoire d'Oncogénétique, UFR Médecine, Hôpital Lariboisière; Université Paris 7, 41 Bd de la chapelle, F-75475 Paris Cedex 10, France

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M Pocard
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I Bieche INSERM U965, Laboratoire d'Oncogénétique, UFR Médecine, Hôpital Lariboisière; Université Paris 7, 41 Bd de la chapelle, F-75475 Paris Cedex 10, France

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M Perrot-Applanat
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effects of V The mechanisms underlying the patterns of gene regulation observed at PND35 and PND50 after exposure to G, V, and GV during gestation and lactation remain to be explored. V is an anti-androgenic compound that can be metabolized to generate two

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Katherine A Staines The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Edinburgh, Midlothian EH25 9RG, UK

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Vicky E MacRae The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Edinburgh, Midlothian EH25 9RG, UK

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Colin Farquharson The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Edinburgh, Midlothian EH25 9RG, UK

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. Journal of Biological Chemistry 280 6197 – 6203 . doi:10.1074/jbc.M412911200 . You J Reilly GC Zhen X Yellowley CE Chen Q Donahue HJ Jacobs CR 2001 Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium

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Zhihao Liu Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Fengrui Wu Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Baowei Jiao Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Xiuyue Zhang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Chongjiang Hu Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Baofeng Huang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Linyan Zhou Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Xigui Huang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Zhijian Wang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Yaoguang Zhang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Yoshitaka Nagahama Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Christopher H K Cheng Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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Deshou Wang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
Department of Biochemistry and the Environmental Science Programme, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

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): cloning, preovulatory induction of gene expression, hormonal gene regulation and analysis of promoter region. Journal of Molecular Endocrinology 35 571 –583. Kazeto Y , Ijiri S, Place AR, Zohar Y & Trant JM 2001 The 5

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Xigui Huang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Baowei Jiao Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Chun Kit Fung Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Yong Zhang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Walter K K Ho Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Chi Bun Chan Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Haoran Lin Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Deshou Wang Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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Christopher H K Cheng Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China
Department of Biochemistry and
The Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

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gene promoter (Fig. 11A ). These strong promoter activities indicated that the cloned 5′-flanking regions did represent the functional promoters of the seabream PRLR genes. Regulation of promoter activities by steroid

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