difference. Results RACK1 hep−/− mice exhibited hepatic steatosis and loss of glycogen Hepatic RACK1 knockout mice (RACK1 hep−/− ) were generated using the Cre-Loxp strategy as the Method and Fig. 1A stated. Hepatic RACK1-deficient mice (RACK
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Wanying Qin, Ting Zhang, Mingxia Ge, Huimin Zhou, Yuhui Xu, Rongfang Mu, Chaoguang Huang, Daowei Liu, Bangrui Huang, Qian Wang, Qinghua Kong, Qingpeng Kong, Fei Li, and Wenyong Xiong
Li Ding, Yue Yin, Lingling Han, Yin Li, Jing Zhao, and Weizhen Zhang
aprotinin were purchased from Sigma Chemical Co. and Amersham Biosciences, respectively. Animals and animal care Ngn3-Cre mice that express the Cre recombinase gene under the control of the Ngn3 gene promoter, as well as Tsc1 loxP/loxP mice
Eugenia H Goulding, Sylvia C Hewitt, Noriko Nakamura, Katherine Hamilton, Kenneth S Korach, and Edward M Eddy
examines mice generated with exon 3 of the Esr1 gene flanked by loxP sites and crossed with Sox2 -cre transgenic mice to produce mice with a deletion in the Esr1 gene of the region encoding the DNA-binding domain (Ex3αERKO) and lacking a functional ERα
Yujiao Dai, Peng Hao, Zhimei Sun, Zhiyi Guo, Hong Xu, Lihui Xue, Hongyu Song, Yida Li, Shuang Li, Mingming Gao, Teng Si, Yuxin Zhang, and Yajuan Qi
Yap mice ( Yap Loxp/Loxp ), albumin-Cre mice, and liver-specific YAP gene knockout (L-YKO) mice (albumin Cre::YAP Loxp/Loxp ) have been previously described ( Schlegelmilch et al. 2011 ). In this experiment, 2-month-old L-YKO mice were used. The
Jessica L Pierce, Ke-Hong Ding, Jianrui Xu, Anuj K Sharma, Kanglun Yu, Natalia del Mazo Arbona, Zuleika Rodríguez-Santos, Paul J Bernard, Wendy B Bollag, Maribeth H Johnson, Mark W Hamrick, Dana L Begun, Xing-Ming Shi, Carlos M Isales, and Meghan E McGee-Lawrence
. 1A ). While inclusion of an additional ‘Cre+ control’ (i.e., GR +/+ :Osx1-Cre+ for this study) is preferred for knockout models generated with the Osx1-Cre line, due to the presence of a mild, early skeletal phenotype arising from the Osx1-Cre itself
David W Scoville, Kristin Lichti-Kaiser, Sara A Grimm, and Anton M Jetten
defined. To gain greater insights into the regulatory functions of GLIS3 in the pancreas, we generated a pancreas-specific Glis3 -knockout mouse model. Glis3 fl/fl mice were crossed with mice expressing Cre recombinase under the control of the Pdx1
Barbara C Fam, Laura J Rose, Rebecca Sgambellone, Zheng Ruan, Joseph Proietto, and Sofianos Andrikopoulos
KO mice were produced using the Cre/loxP system. The construct used for the generation of the mice has been previously reported ( Kaczmarczyk et al . 2003 ). Heterozygous floxed Glut4 mice, with the PGK-NeoR selection cassette inserted at the end
Johan Svensson, Jon Kindblom, Ruijin Shao, Sofia Movérare-Skrtic, Marie K Lagerquist, Niklas Andersson, Klara Sjögren, Katrien Venken, Dirk Vanderschueren, John-Olov Jansson, Olle Isaksson, and Claes Ohlsson
IGF1 is liver derived ( Sjögren et al . 1999 , Yakar et al . 1999 ). A mouse model with liver-specific, inducible inactivation of the Igf1 gene, using the Cre-LoxP conditional knockout system, has been developed (LI-IGF1 −/− mice; Sjögren et al
K L Gustafsson, K H Nilsson, H H Farman, A Andersson, V Lionikaite, P Henning, J Wu, S H Windahl, U Islander, S Movérare-Skrtic, K Sjögren, H Carlsten, J-Å Gustafsson, C Ohlsson, and M K Lagerquist
.2015 ) 27807202 10.1152/physrev.00033.2015 Antonson P Omoto Y Humire P Gustafsson JA 2012 Generation of ERalpha-floxed and knockout mice using the Cre/LoxP system . Biochemical and Biophysical Research Communications 424 710 – 716 . ( https
Abigail Wolf Greenstein, Neena Majumdar, Peng Yang, Papasani V Subbaiah, Rhonda D Kineman, and Jose Cordoba-Chacon
)-specific impact of PPARγ on lipid homeostasis. With respect to the hepatocyte, studies using hepatocyte-specific PPARγ-knockout models have led to the conclusion that PPARγ directly promotes hepatic fat accumulation by increasing lipid uptake, as well as promoting
