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. Liu F Austin DA Mellon PL Olefsky JM Webster NJ 2002 GnRH activates ERK1/2 leading to the induction of c-fos and LHβ protein expression in LβT2 cells . Molecular Endocrinology 16 419 – 434 . Lou J Tu Y Li S Manske PR 2000 Involvement
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China
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Shandong Sport Training Center, Jinan, China
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Soares AF Lagarde M Geloen A 2008 Lou/C obesity-resistant rat exhibits hyperactivity, hypermetabolism, alterations in white adipose tissue cellularity, and lipid tissue profiles . Endocrinology 149 615 – 625 . ( doi:10.1210/en.2007
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used for all experimental procedures and were maintained on a 12 h light:12 h darkness cycle in a temperature (22°C) and humidity controlled (45–55%) environment with access to food and water ad libitum . SerpinA3N floxed mice on a C57BL6/J:129S
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gassed with 95% air, 5% CO 2 at 37 °C. Medium was refreshed every 7 days until cells reached confluence (2–4 weeks). A further advantage of 2- to 4-week cell culture in FBS-containing medium before experimentation was neutralisation of any disparity of
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experiments were response time of 2s, bandwidth of 1nm, scanning speed of 50nm/min, and a data pitch of 0.2nm. All spectra were acquired at 25°C by accumulation of 15 scans in a 1mm quartz cell, and the baseline corrected. Calculation of alpha-helical and beta
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School of Science and Technology, Nottingham Trent University, Nottingham, UK
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Pediatric Research Center, Hospital for Child and Adolescent Medicine, Leipzig University, Leipzig, Germany
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al. 2019 ) were conducted with male C57BL/6J or C57BL/6JRj mice (B6J), a commonly used mouse model to study the effects of diet-induced obesity and glucose intolerance. A limitation of using these mice for metabolic studies is a deficiency in
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peptide PVN (mRNA) Arc (mRNA) TSH TT 4 TT 3 Cort References Trh Crh Agrp Npy Cart Pomc Bdnf Lou/C rat vs Wistar ↓ + ↑ * ↑ * ↑ * – + ↓ + ↑ ↑ ↓ – ↓ Mitchell et al
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receptor function by constitutively active protein kinase C . Molecular Pharmacology 54 1055 – 1063 . ( doi:10.1124/mol.54.6.1055 ) Yan JE Yuan W Lou X Zhu T 2012 Streptozotocin-induced diabetic hyperalgesia in rats is associated with
Clinical Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
YMU-HKBU Joint Laboratory of Traditional Natural Medicine, Yunnan Minzu University, Kunming, China
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YMU-HKBU Joint Laboratory of Traditional Natural Medicine, Yunnan Minzu University, Kunming, China
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.5 to 2.0 kg were purchased from local markets and kept in a well-aerated 200-L aquaria under a 12-h L:12-h D photoperiod at 18 ± 2°C. The fish were sacrificed under anesthesia with 0.05% tricaine methanesulfonate (Sigma) followed by spinosectomy
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% air at 37 °C. Upon reaching monolayer confluence, the cells were split by treatment with 0.25% trypsin solution to give a final concentration of 5×10 4 cells/ml with PBS before starvation in 0.1% BSA (fraction V, Sigma), phenol red-free and serum