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W. B. WATKINS
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Three neurophysins have been isolated and biochemically characterized from the ox (Rauch, Hollenberg & Hope, 1969), pig (Uttenthal & Hope, 1970) and sheep (Watkins, 1972) while the presence of a neurophysin has been demonstrated in cod pituitary glands (Pickering, 1968).

By means of immunological and biosynthesis techniques, Norstrom, Sjöstrand, Livett, Uttenthal & Hope, (1971) have tentatively identified one neurophysin in the rat posterior pituitary gland. In a recent extension of this work, Burford, Jones & Pickering (1971) using polyacrylamide electrophoresis have been able to resolve the major rat posterior pituitary gland protein into two major components one of which was tentatively described as an oxytocin-neurophysin and the other as a vasopressin—neurophysin.

In this present investigation, the neurophysins of the rat were fractionated using procedures previously established for the purification of the ox, pig and sheep neurophysins. The protein-hormone complex was prepared in a yield of 26·9 mg from an

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Angélica Morales
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Sumiko Morimoto
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Lorenza Díaz
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Guillermo Robles Departamento de Biología de la Reproducción, Departamento de Medicina Experimental, MEXFAM A.C. Juárez 208 Tlalpan, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Vasco de Quiroga # 15 Tlalpan, 14000 México DF, Mexico

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Vicente Díaz-Sánchez Departamento de Biología de la Reproducción, Departamento de Medicina Experimental, MEXFAM A.C. Juárez 208 Tlalpan, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Vasco de Quiroga # 15 Tlalpan, 14000 México DF, Mexico

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-del Castillo et al . 1991 ), we hypothesized that testosterone could participate in the regulation of EG-VEGF expression. This work was undertaken to 1) study the expression and regulation of EG-VEGF in the rat pancreas under different endocrine conditions, 2

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Mary C Gieske Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Gi Youn Na Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Yongbum Koo Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Misung Jo Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Thomas E Curry Jr Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Chemyong Ko Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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developing follicles, while CD59 is only localized to granulosa and theca cells of developing follicles ( Oglesby et al. 1996 ). In the rat, DAF protein was reported to be present in the endothelium of the ovary ( Spiller et al. 1999 ). DAF provides the

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Ichiro Sakata Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyaki-dai, Saitama 350-02, Japan

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Toru Tanaka Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyaki-dai, Saitama 350-02, Japan

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Mami Yamazaki Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyaki-dai, Saitama 350-02, Japan

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Takashi Tanizaki Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyaki-dai, Saitama 350-02, Japan

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Zhao Zheng Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyaki-dai, Saitama 350-02, Japan

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Takafumi Sakai Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyaki-dai, Saitama 350-02, Japan

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( Ackerman et al. 1981 , Nelson & Bulun 2001 ). Recently, Ueyama et al. (2002) clearly demonstrated that estrogen synthetase (aromatase) is expressed in gastric parietal cells of the rat stomach. Furthermore, Campbell-Thompson et al. (2001) reported

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Muhtashan S Mondal Division of Neurology, Respirology, Endocrinology, and Metabolism, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

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Koji Toshinai Division of Neurology, Respirology, Endocrinology, and Metabolism, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

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Hiroaki Ueno Division of Neurology, Respirology, Endocrinology, and Metabolism, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

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Keiichi Koshinaka Division of Neurology, Respirology, Endocrinology, and Metabolism, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

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Masamitsu Nakazato Division of Neurology, Respirology, Endocrinology, and Metabolism, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

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Introduction Ghrelin, a 28-amino acid peptide with an n -octanoyl modification, was originally discovered in human and rat stomach as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R; Kojima et al . 1999 ). Ghrelin is

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A Santillo Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi, 43, 81100 Caserta, Italy

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L Burrone Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi, 43, 81100 Caserta, Italy

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S Falvo Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi, 43, 81100 Caserta, Italy

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R Senese Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi, 43, 81100 Caserta, Italy

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A Lanni Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi, 43, 81100 Caserta, Italy

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G Chieffi Baccari Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi, 43, 81100 Caserta, Italy

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Introduction The Harderian gland (HG) is a large tubuloalveolar gland present in the orbit of most terrestrial vertebrates (see Chieffi et al . 1996 for review). In the rat, HG excretes large amounts of characteristic lipids, and it also produces

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Ruben Nogueiras Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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Sulay Tovar Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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Sharon E Mitchell Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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Perry Barrett Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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D Vernon Rayner Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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Carlos Dieguez Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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Lynda M Williams Department of Physiology, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Obesity and Metabolic Health Division, Rowett Research Institute, Aberdeen AB21 9SB, UK

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hypothalamus ( Graham et al. 2003 ). These nuclei are important in controlling circadian rhythms, appetite and energy balance ( Takahashi & Zatz 1982 , Williams et al. 2001 ). In contrast, NMU expression in the rat is limited to the SCN, relatively few

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Nguyen Khanh Hoa Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Åke Norberg Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Rannar Sillard Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Dao Van Phan Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Nguyen Duy Thuan Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Dang Thi Ngoc Dzung Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Hans Jörnvall Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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Claes-Göran Östenson Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Institute of Material Medica, Hanoi, Vietnam
Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden

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W rat islets were incubated with phanoside and the PKA-inhibitor, H89 (10 μM; Filipsson & Ahren 1998 ) or the PKC inhibitor, calphostin-C (1.5 μM; Thams & Capito 2001 ) for 60 min in KRB containing 3.3 or 16.7 mM glucose with or without phanoside

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K P Lai Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, People’s Republic of China

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M H Wong Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, People’s Republic of China

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C K C Wong Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, People’s Republic of China

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volume and number ( Johnson et al. 1992 , 1994 , Wilker et al. 1995 ). Detrimental effects on Sertoli and germ cells of rat testes, such as reduction of the intercellular contact of neighboring cells, disruption of germ cell development, decrease of

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Juthamard Surapongchai Exercise Physiology Laboratory, Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand

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Mujalin Prasannarong Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand

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Tepmanas Bupha-Intr Exercise Physiology Laboratory, Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand

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Vitoon Saengsirisuwan Exercise Physiology Laboratory, Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand

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18:00 h), and the animals had free access to water and pellet rat chow (Perfect Companion, Samutprakarn, Thailand). The rats were randomly assigned to either a sham operation (SHAM, n  = 10) or chronic ANGII infusion ( n  = 20). Subcutaneous

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