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Facultad de Química and
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacan 04510, D F México, México
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Facultad de Química and
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacan 04510, D F México, México
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Facultad de Química and
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacan 04510, D F México, México
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Facultad de Química and
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacan 04510, D F México, México
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Facultad de Química and
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacan 04510, D F México, México
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Facultad de Química and
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacan 04510, D F México, México
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:100) (Jackson Immunoresearch Laboratories, Wets Grove, PA, USA). After this procedure, sections were incubated for 4 h with mouse anti-rat glucagon (1:6000) (Sigma) and a second CY5-conjugated goat anti-mouse IgG (1:100) (Jackson Immunoresearch Laboratories
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the level of the male genital tract. The primary goal of the present study was to extend our previous observations obtained in other species ( Vignozzi et al. 2004 ) to the rat and to verify whether or not OT has any effect in an in vivo
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Introduction Type 1 diabetes (T1D) is an autoimmune disease characterized by immunocyte infiltration of the pancreatic islets (insulitis) and destruction of the insulin-secreting β cells. Diabetes in the biobreeding (BB) rat exhibits many
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signal during the LH surge is GnRH self-priming, and we have shown for rat gonadotropes that there is overlap of the self-priming pathway with activation of the progesterone receptor (PR; Waring & Turgeon 1992 , Turgeon & Waring 1994 ). For mouse
Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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Department of Anatomy, Showa University School of Medicine, Tokyo 142-8555, Japan
Discovery Research Laboratories, Pharmaceuticals Research Division, Takeda Chemical Industries, Ibaraki 300-4293, Japan
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% nucleotide and 64% amino acid identity ( O’Dowd et al. 1995 ). While orthologs of GPR7 and GPR8 have been found in multiple species by PCR, a rodent GPR8 has yet to be identified ( Lee et al. 1995 ). Rat GPR7 mRNA is widely expressed throughout the brain
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H6PD using rat adipose and liver tissues and 3T3-L1 adipocytes. Materials and methods Materials Steroid standards were obtained from Steraloids, Inc. (Newport, RI, USA); 3-( N -morpholino)propanesulfonic (MOPS) acid from Sigma; NADP, NADPH, G6P, and
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al. 1996 ). Rat UCN is a 40-amino-acid peptide with 45% sequence identity to CRH ( Vaughan et al. 1995 ), 95% amino-acid identity with human UCN ( Donaldson et al. 1996 ) and 63% identity with the fish peptide, urotensin ( Vaughan et al
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Introduction As a member of the corticotropin-releasing factor (CRF) family peptides, urocortin (Ucn 1) was discovered by screening of a rat brain cDNA library with a probe derived from carp urotensin I ( Vaughan et al. 1995
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D1 mRNA expression in rat hepatocytes ( Menjo et al. 1993 , Davies et al. 1996 ). So far it remains unclear as to what is the cause behind this discrepancy. Also in NRK-52E kidney cells, dexamethasone (DEX) increases D1 activity and mRNA
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Laboratory for Reproductive Health, Palo Alto Institute for Research and Education, Guangdong Key Laboratory of Nanomedicine, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
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)-induced testosterone production in rat testis, while resistin-stimulated testosterone production in rat testis ( Tena-Sempere et al . 1999 , Nogueiras et al . 2004 ). Recent reports point to a role of the novel adipokine chemerin in reproduction as well, focusing on