arthritis on visceral WAT mass regulation. Serum concentrations of leptin and adiponectin as well as their gene expression in WAT were measured as they are the main adipokines released by the adipose tissue. As lipolytic factors, HSL and ZAG gene expression
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A I Martín, E Castillero, M Granado, M López-Menduiña, M A Villanúa, and A López-Calderón
May Faraj, Genevieve Beauregard, Emmanuelle Loizon, Marthe Moldes, Karine Clément, Youssef Tahiri, Katherine Cianflone, Hubert Vidal, and Rémi Rabasa-Lhoret
-derived proteins by insulin infusion. The WAT-derived proteins examined were hexokinase II (HK II), the regulatory subunit p85α of phosphatidylinositol-3 kinase (PI3K), leptin, adiponectin, and acylation-stimulating protein (ASP). These proteins have been well
Miriam Thomalla, Andreas Schmid, Elena Neumann, Petra Ina Pfefferle, Ulf Müller-Ladner, Andreas Schäffler, and Thomas Karrasch
adipocytes, cells were harvested and mRNA was isolated applying RNeasy Mini Kit according to manufacturer’s recommendations. Quantification of adiponectin, GLUT1, GLUT4, IL-6, MCP-1 and TLR9 mRNA expression in murine 3T3-L1 adipocytes in vitro and ex
Elaine de Oliveira, Egberto G Moura, Ana Paula Santos-Silva, Cíntia R Pinheiro, Natalia S Lima, José Firmino Nogueira-Neto, Andre L Nunes-Freitas, Yael Abreu-Villaça, Magna C F Passos, and Patrícia C Lisboa
as leptin and adiponectin, with autocrine, paracrine, and endocrine functions ( Matsuzawa 2006 ). Adiponectin production is inversely proportional to whole-body adipose mass, and experimental studies suggest that adiponectin increases insulin
Barbara C Fam, Rebecca Sgambellone, Zheng Ruan, Joseph Proietto, and Sofianos Andrikopoulos
was collected and stored at 4 °C for the measurement of leptin, adiponectin (total) and ghrelin (acylated) (one assay using the Mouse Gut Hormone MILLIplex kit, Millipore), insulin (mouse-specific ELISA; 80-INSMS-E01, ALPCO Diagnostics, Salem, NH, USA
M Fraenkel, J Caloyeras, S-G Ren, and S Melmed
measured in samples collected during glucose loading using an ultra-sensitive rat ELISA kit (CrystalChem, Downers Grove, IL, USA); fasting concentrations of adiponectin were measured using a mouse adiponectin RIA kit (Linco Research, St Charles, MO, USA
Fernando Escrivá, M Lucía Gavete, Yasmín Fermín, Coralia Pérez, Nilda Gallardo, Carmen Alvarez, Antonio Andrés, Manuel Ros, and José M Carrascosa
, adiponectin, and leptin have also been analyzed. Materials and Methods Animals Male Wistar rats of 3, 8, and 24 months old from our in-house colony (Centre of Molecular Biology, Madrid, Spain) were housed in climate
Jose A Viscarra, Cory D Champagne, Daniel E Crocker, and Rudy M Ortiz
.e. type 2 diabetes mellitus) ( Campbell 2009 , Lin & Sun 2010 ). Various hormones and proteins, such as adiponectin, glucagon-like peptide-1 (GLP-1), insulin-like growth factor 1 (IGF1), and peroxisome proliferator-activated receptor γ (PPARγ), are
Tracy Josephs, Hayley Waugh, Ilona Kokay, David Grattan, and Mary Thompson
adipokines that serve as feedback signals to regulate WAT metabolism as well as having effects on food intake, energy expenditure, and carbohydrate and lipid metabolism ( Trayhurn 2005 , Ronti et al. 2006 ). These adipokines include leptin, adiponectin
Simon J Dunmore and James E P Brown
) expression ratio, which may also explain the reduction in apoptosis induced by this factor ( Brown & Dunmore 2007 ). By contrast, a recent study by Chetboun et al . (2012) found no effect of leptin (or adiponectin) on apoptosis, although both adipokines
