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Core-binding factor A1 (Cbfa1), also called Pebp2 alpha A/AML3, is a transcription factor that belongs to the runt-domain gene family. Cbfa1-deficient mice are completely incapable of both endochondral and intramembranous bone formation, indicating that Cbfa1 is indispensable for osteogenesis. Maturation of chondrocytes in these mice is also disorganized, suggesting that Cbfa1 may also play a role in chondrogenesis. The aim of this study was to examine the expression and regulation of Pebp2 alpha A/AML3/Cbfa1 expression in the chondrocyte-like cell line, TC6. Northern blot analysis indicated that Cbfa1 mRNA was constitutively expressed as a 6.3 kb message in TC6 cells and the level of Cbfa1 expression was enhanced by treatment with bone morphogenetic protein-2 (BMP2) in a time- and dose-dependent manner. This effect was blocked by an RNA polymerase inhibitor, 5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole, but not by a protein synthesis inhibitor, cycloheximide. Western blot analysis of the cell lysates using polyclonal antibody raised against Cbfa1 indicated that BMP2 treatment increased the Cbfa1 protein level in TC6 cells. In TC6 cells, BMP2 treatment enhanced expression of alkaline phosphatase and type I collagen mRNAs but suppressed that of type II collagen mRNA. In addition to TC6 cells, Cbfa1 mRNA was also expressed in primary cultures of chondrocytes and BMP2 treatment enhanced Cbfa1 mRNA expression in these cells similarly to its effect on TC6 cells. These data indicate that the Pebp2 alpha A/AML3/Cbfa1 gene is expressed in a chondrocyte-like cell line, TC6, and its expression is enhanced by treatment with BMP.
Lee Gil Ya Cancer and Diabetes Institute, Department of Endocrinology and Metabolism, Howard Hughes Medical Institute, Gachon Medical Research Institute, Department of Rehabilitation Medicine, College of Pharmacy, Gachon University, 7-45 Songdo‐dong, Yeonsu‐ku, Incheon, Korea
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Lee Gil Ya Cancer and Diabetes Institute, Department of Endocrinology and Metabolism, Howard Hughes Medical Institute, Gachon Medical Research Institute, Department of Rehabilitation Medicine, College of Pharmacy, Gachon University, 7-45 Songdo‐dong, Yeonsu‐ku, Incheon, Korea
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known as the unfolded protein response, which involves double-stranded RNA-activated protein kinase RNA-like kinase (PERK), activating transcription factor 6 (ATF6), and inositol requiring enzyme 1 (IRE1) pathways ( Wu & Kaufman 2006 , Lai et al . 2007
Department of Biochemistry, Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong SAR, China
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Department of Biochemistry, Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong SAR, China
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Department of Biochemistry, Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong SAR, China
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Department of Biochemistry, Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong SAR, China
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Department of Biochemistry, Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong SAR, China
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complexes through interactions with a large repertoire of transcription factors and components of basal transcription machinery ( Chan & La Thangue 2001 , Vo & Goodman 2001 ). The intrinsic acetyltransferase activity of p300 augments the activation of gene
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specific transcription factors, co-factors, and adaptor proteins. The activity of GR is modulated by post-transcriptional modifications, similar to C/EBPs, and the GR is involved in complex cross-talk with other signaling pathways, for instance nuclear
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this disease every year ( Ferlay et al. 2015 ). Approximately 70% of breast tumors are estrogen receptor α (ERα) positive, and tumor cell proliferation is thought to be dependent on the activity of this hormone-mediated transcription factor ( Hayashi
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Introduction The transcriptional networks driving mammalian cell development and function are only beginning to be elucidated. In many tissues transcription factors critical to normal development and function have been identified but, in general
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Cell and Developmental Biology, Departments of
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target genes required for proper islet cell function. MafA and MafB were only recently linked to Insulin and Glucagon expression and represent the principal members of the large Maf transcription factor family expressed in the pancreas ( Olbrot et al
Department of Physiology and Biophysics, Interdisciplinary Biomedical Sciences Program, Arkansas Children's Nutrition Center, Buck Institute for Age Research, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, Arkansas 72202, USA
Department of Physiology and Biophysics, Interdisciplinary Biomedical Sciences Program, Arkansas Children's Nutrition Center, Buck Institute for Age Research, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, Arkansas 72202, USA
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Department of Physiology and Biophysics, Interdisciplinary Biomedical Sciences Program, Arkansas Children's Nutrition Center, Buck Institute for Age Research, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, Arkansas 72202, USA
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Department of Physiology and Biophysics, Interdisciplinary Biomedical Sciences Program, Arkansas Children's Nutrition Center, Buck Institute for Age Research, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, Arkansas 72202, USA
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in the pathobiology of uterine endometrial and breast cancers. Figure 1 Cladogram of the human Sp and KLF transcription factors. The 110-aa domain containing the buttonhead box (BTD)/zinc finger motifs was used for the multiple alignment with ClustalW
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implicated it in processes of inflammation, atherosclerosis, cell cycle control, apoptosis, and carcinogenesis ( Auwerx 1999 ). PPARs act as a transcription factor when heterodimerized with the retinoid X receptor and bound to the proper ligands, including
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RANKL. RANKL is the ligand for receptor activator of nuclear factor-kappaB (RANK) and is induced by progesterone ( Fata et al. 2000 , Brisken et al. 2002 ). Pax-2 is a proto-oncogene transcription factor characterized by a paired domain and
