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Olson Center for Women’s Health, Department of Obstetrics and Gynecology, and Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, 985860 Nebraska Medical Center, Omaha, Nebraska, USA
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as follows: Ki67 (ab833-500, 1:100) from Abcam; p-H3 (3377S, 1:50), γH2AX (9718s, 1:100), phospho-p63 (4981s, 1:50), cleaved PARP (9548s, 1;100), phospho-ATR (2853T, 1:50), phospho-CHK1 (12302T, 1:50), phospho-CHK2 (2197S, 1:50) and phospho-AKT (9271s
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/mL aprotinin). Akt phosphorylation assay An i.p. administration of 100μL (10U) of insulin (Humulin, Eli Lilly) was performed in all groups (CTL, DIO, and DIO+EXE). Some of the CTL mice received 100μL of saline solution, instead of insulin, as described
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-195), cdk1 (06-141), and cdk2 (05-596) were purchased from Upstate (Temecula, CA, USA); antibodies to cyclin A (sc-596), cyclin B1 (sc-595), and cytokeratin 18 (sc-28264) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); antibodies to AKT (9272) and
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), anti-PLC-β2 (rabbit polyclonal, sc-9018), anti-Akt1 (rabbit polyclonal, sc-8312), anti P70S6K (mouse monoclonal sc-8418) anti-phospho Akt1 -Ser473 (rabbit polyclonal, sc-7985), anti-MAPK3 (rabbit polyclonal, sc-94), anti-MAPK 2 (rabbit polyclonal, sc
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42 MAPK), p38 MAPK, the PKC, the PI3K/AKT, and the STAT pathways, involved in cell proliferation, survival, and motility ( Jorissen et al . 2003 , Henson & Gibson 2006 , Normanno et al . 2006 ). ErbB receptors are activated not only by direct
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India
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Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India
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Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India
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Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India
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). Moreover, mTOR regulates autophagy involving ULK1 phosphorylation and phosphoinositide 3-kinase (PI3K)-AKT activation, which associates with apoptosis as well ( Zhang & Wang 2018 , Pandey et al. 2020 ). We reported that dysregulated autophagy mechanism
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Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA
Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa City, Iowa, USA
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fat tissues were collected and snap frozen 15 min after injection. Immunodetection Tissues were homogenized on ice and centrifuged at 10,000 g at 4°C for 4 min. Supernatant content of AKT/phospho-AKT, PDK1/phosphor-PDK1, MTOR
Department of Physiological Sciences, State University of Londrina, Londrina, Brazil
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Biotechnology Unit, University of Ribeirao Preto, Ribeirao Preto, Brazil
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Department of Physiological Sciences, Biomedical Sciences Institute, Federal University of Alfenas, Alfenas, Brazil
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tyr1222 (1:1500, Cell Signaling # 3066), rabbit anti β-actin (1:1000, Cell Signaling # 8457), rabbit anti-AKT (1:10000, Cell Signaling # 4691); rabbit anti-phospho AKT S473 (1:1500, Cell signaling # 9271), rabbit anti-JNK (1:5000, Cell Signaling # 9252
Department of Endocrinology, FuJian Union hospital, Fuzhou, P R China
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has been proposed to upregulate the expression of insulin receptor substrate 2 (IRS2) through increased cytosolic calcium levels and then activate the PI3K/AKT pathway to induce β-cell proliferation ( Jhala et al . 2003 , Heit et al . 2006 , Porat
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. 2007 ). For example genistein, one of the phytoestrogens, affects transcriptional activity of ESR1 and ESR2 by modulating their binding affinity to estrogen-response elements (ERE) and inhibiting activity of both the PI3K/AKT and MAPK cell signaling