controlled the rise of hypothalamic GnRH production before puberty in mice ( Messina et al. 2016 ). These results suggested the existence of a multilayered and interconnected array of miRNAs and their target genes which control puberty timing ( Lomniczi et
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Yuxun Zhou, Li Tong, Maochun Wang, Xueying Chang, Sijia Wang, Kai Li, and Junhua Xiao
Weiliang Xia, Dolores D Mruk, Will M Lee, and C Yan Cheng
curve. Relative expression of Stat3 was normalized to the S-16 control for each sample. For comparative C T method, the difference of C T between the target gene and S-16 for each sample was calculated (Δ C T ) and then this difference for other
Ana Sofia Rocha, Ricardo Marques, Inês Bento, Ricardo Soares, João Magalhães, Inês Vieira de Castro, and Paula Soares
to be involved in the development of papillary thyroid carcinoma (PTC). Rearrangements of the RET tyrosine kinase receptor gene (RET/PTC) are found in 13–43% and the V600E BRAF in 29–69% of sporadic PTC ( Kondo et al. 2006 ). Targeted expression of
Gregory S Y Ong, Timothy J Cole, Gregory H Tesch, James Morgan, Jennifer K Dowling, Ashley Mansell, Peter J Fuller, and Morag J Young
aldosterone-induced gene expression in iBMDMs derived from the MyMRC603S mice to evaluate MR-DNA binding responses. In WT iBMDMs, aldosterone (10 nM)-induced gene transcription showed two patterns of response for MR target genes: at 2 h and 6 h glucocorticoid
Xiaoning Li, Junhua Xiao, Yating Fan, Kan Yang, Kai Li, Xin Wang, Yanhua Lu, and Yuxun Zhou
-knockout GT1-7 cells, the level of Gnrh1 expression increased by two-fold. Tbx21 , a target gene of miR-29 family, acts as a transcriptional activator of Gnrh1 in this process, TBX21 also activates the expression of Dlx1 , another activator of Gnrh1
Sean C Lema, Jon T Dickey, Irvin R Schultz, and Penny Swanson
( Matta et al . 2002 ). While these and other studies have established diverse roles for THs in the growth, development, and behavior of fish (reviewed by Power et al . 2001 , Yamano 2005 ), little is known about gene targets for TH action in teleosts
Robert L Moore and Douglas V Faller
of estrogen-regulated genes. As chemical inhibitors are never completely specific with respect to their enzymatic target, more specific, genetically based methods of inhibiting SIRT1 activity were also used. Cells were transfected with a dominant
Marika Bogdani, Angela M Henschel, Sanjay Kansra, Jessica M Fuller, Rhonda Geoffrey, Shuang Jia, Mary L Kaldunski, Scott Pavletich, Simon Prosser, Yi-Guang Chen, Åke Lernmark, and Martin J Hessner
(SAM) software as described ( Tusher et al . 2001 ). More stringent statistical criteria were not applied, as relevant genes and pathways would be investigated further by quantitative RT-PCR (qRT-PCR) and targeted follow-up studies. Ontological pathway
Sílvia Emiko Matsuo, Suzana Garcia Leoni, Alison Colquhoun, and Edna Teruko Kimura
, TX, USA), which contains the H1 RNA polymerase III promoter to drive siRNA expression and a puromycin resistant gene to enable antibiotic selection in mammalian cells. To design siRNA, three mRNA target sequences were selected for TGF- β1 RNAi (1
N Zlocowski, L d V Sosa, B De la Cruz-Thea, C B Guido, M G Martín, J H Mukdsi, A I Torres, and J P Petiti
become increasingly significant as a result of its identified role in the regulation of tumor progression, as well as its ability to target the genes involved in this process pharmacologically ( Miranda Furtado et al. 2019 , Eich et al. 2020
