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Tomoko Minamizaki Department of Calcified Tissue Biology, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

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Yukiko Konishi Department of Calcified Tissue Biology, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan
Department of Pediatric Dentistry, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

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Kaoru Sakurai Department of Calcified Tissue Biology, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan
Department of Pediatric Dentistry, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

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Hirotaka Yoshioka Department of Calcified Tissue Biology, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

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Jane E Aubin Department of Molecular Genetics, University of Toronto, 1 King’s College Circle, Toronto, Canada

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Katsuyuki Kozai Department of Pediatric Dentistry, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

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Yuji Yoshiko Department of Calcified Tissue Biology, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

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eosin or immunofluorescence staining. Frontal sections (thickness, 5 µm), approximately 2-mm from the sagittal suture, were used for histomorphometry. Sections pretreated with Dako protein block (Dako) were incubated with a mixture of polyclonal

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Natalie K Y Wee Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia

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Thaísa F C de Lima Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia
Department of Genetics and Molecular Biology, University of Campinas, São Paulo, Brazil

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Narelle E McGregor Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia

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Emma C Walker Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia

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Ingrid J Poulton Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia

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Martha Blank Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia
Department of Medicine, The University of Melbourne, St. Vincent’s Hospital, Melbourne, Australia

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Natalie A Sims Bone Cell Biology and Disease Unit, St Vincent’s Institute of Medical Research, Fitzroy, Australia
Department of Medicine, The University of Melbourne, St. Vincent’s Hospital, Melbourne, Australia

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to sex-matched control mice. All microCT measurements, histomorphometry, and mechanical testing were conducted with the observers blinded to the sex and genotype of the samples. Results Dmp1cre -targeted Leptin receptor deficiency under

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Bronwen A J Evans School of Biosciences, Department of Child Health, Division of Molecular Neuroendocrinology, Department of Anatomy, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK

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Melanie J Bull
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Rebecca C Kench
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Rebecca E Fox
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Liam D Morgan School of Biosciences, Department of Child Health, Division of Molecular Neuroendocrinology, Department of Anatomy, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK

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Amy E Stevenson
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Evelien F Gevers School of Biosciences, Department of Child Health, Division of Molecular Neuroendocrinology, Department of Anatomy, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK

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Mark J Perry School of Biosciences, Department of Child Health, Division of Molecular Neuroendocrinology, Department of Anatomy, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK

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Timothy Wells
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(D)) and organisation (pattern factor (E)) were assessed by μ-CT and are shown together with osteoblast surface (assessed by histomorphometry) in the proximal (F) and distal (G) tibiae. Values shown are mean± s.e.m. ( n =6 for both groups); with

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Louise Grahnemo Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Caroline Jochems Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Annica Andersson Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Cecilia Engdahl Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Claes Ohlsson Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Ulrika Islander Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Hans Carlsten Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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is the use of serum markers as a measure of bone turnover, instead of histomorphometry. Another limitation is the lack of data on trabecular bone strength, as the three-point bending test used in this study mainly reflects cortical strength. In this

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M Alexandra Sorocéanu
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Dengshun Miao
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Xiu-Ying Bai
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Hanyi Su
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David Goltzman
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Andrew C Karaplis
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group. In rosiglitazone- treated mice the trabeculae were thinner and an increase in trabecular separation was observed. In parallel, an increase in the bone marrow space was apparent. When quantified by histomorphometry, a statistically significant

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Marina Komrakova
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Carsten Krischek Department of Trauma Surgery and Reconstructive Surgery, Institute of Food Quality and Safety, Department of Animal Sciences, University of Goettingen, Robert-Koch 40, 37075 Goettingen, Germany

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Michael Wicke Department of Trauma Surgery and Reconstructive Surgery, Institute of Food Quality and Safety, Department of Animal Sciences, University of Goettingen, Robert-Koch 40, 37075 Goettingen, Germany

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Stephan Sehmisch
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Mohammad Tezval
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Maximillian Rohrberg
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Thomas Brandsch
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Klaus M Stuermer
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Ewa K Stuermer
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effects of programmed administration of human parathyroid hormone fragment (1–34) on bone histomorphometry and serum chemistry in rats . Endocrinology 138 4607 – 4612 doi:10.1210/en.138.11.4607 . Faloona GR Srere PA 1969

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Shun-Neng Hsu The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK
Division of Nephrology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan

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Louise A Stephen The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK

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Scott Dillon The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK

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Elspeth Milne The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK

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Behzad Javaheri Comparative Biomedical Sciences, The Royal Veterinary College, London, UK

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Andrew A Pitsillides Comparative Biomedical Sciences, The Royal Veterinary College, London, UK

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Amanda Novak The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK

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Jose Luis Millán Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA

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Vicky E MacRae The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK

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Katherine A Staines Centre for Stress and Age-Related Disease, University of Brighton, Brighton, UK

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Colin Farquharson The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, UK

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.4) for 14 days at 4°C and processed in paraffin wax. Sections were stained using Goldner’s Trichrome and reacted for tartrate-resistant acid phosphatase. Bone histomorphometry was quantified using the BioQuant Osteo software (BIOQUANT Image Analysis

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Juana Enríquez Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Ana Elena Lemus Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Jesús Chimal-Monroy Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Higinio Arzate Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Gustavo A García Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Bertha Herrero Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Fernando Larrea Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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Gregorio Pérez-Palacios Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición S. Zubirán (INCMNSZ), Mexico City 14000, Mexico
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico

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effectiveness on osteoblasts function are in line with the observation that a female murine model, carrying a null mutation of the PR gene, has not apparent alterations in peak bone mass or bone histomorphometry, thus demonstrating that PR does not play a

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Guillaume Mabilleau Groupe d’Etudes Remodelage Osseux et bioMatériaux, GEROM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France
Bone Pathology Unit, Angers University Hospital, Angers Cedex, France

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Benoit Gobron Groupe d’Etudes Remodelage Osseux et bioMatériaux, GEROM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France
Rheumatology Department, Angers University Hospital, Angers Cedex, France

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Aleksandra Mieczkowska Groupe d’Etudes Remodelage Osseux et bioMatériaux, GEROM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France

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Rodolphe Perrot Service Commun d’Imageries et d’Analyses Microscopiques, SCIAM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France

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Daniel Chappard Groupe d’Etudes Remodelage Osseux et bioMatériaux, GEROM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France
Bone Pathology Unit, Angers University Hospital, Angers Cedex, France
Service Commun d’Imageries et d’Analyses Microscopiques, SCIAM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France

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.jtemb.2013.05.004 Dempster DW Compston JE Drezner MK Glorieux FH Kanis JA Malluche H Meunier PJ Ott SM Recker RR Parfitt AM 2013 Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR

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Giuseppina Maccarinelli
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Valeria Sibilia
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Antonio Torsello
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Francesca Raimondo
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Marina Pitto
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Andrea Giustina
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Carmela Netti
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Daniela Cocchi
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the rat. State of mineral metabolism and bone histomorphometry. European Surgical Research 29 209 –221. Sibilia V , Cocchi D, Pagani F, Lattuada N, Moro GL, Pecile A, Rubinacci AM, Muller EE & Netti C 1999 Hexarelin

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