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Department of Pediatric Dentistry, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan
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Department of Pediatric Dentistry, School of Dentistry, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan
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eosin or immunofluorescence staining. Frontal sections (thickness, 5 µm), approximately 2-mm from the sagittal suture, were used for histomorphometry. Sections pretreated with Dako protein block (Dako) were incubated with a mixture of polyclonal
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Department of Genetics and Molecular Biology, University of Campinas, São Paulo, Brazil
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Department of Medicine, The University of Melbourne, St. Vincent’s Hospital, Melbourne, Australia
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Department of Medicine, The University of Melbourne, St. Vincent’s Hospital, Melbourne, Australia
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to sex-matched control mice. All microCT measurements, histomorphometry, and mechanical testing were conducted with the observers blinded to the sex and genotype of the samples. Results Dmp1cre -targeted Leptin receptor deficiency under
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(D)) and organisation (pattern factor (E)) were assessed by μ-CT and are shown together with osteoblast surface (assessed by histomorphometry) in the proximal (F) and distal (G) tibiae. Values shown are mean± s.e.m. ( n =6 for both groups); with
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Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
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Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
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is the use of serum markers as a measure of bone turnover, instead of histomorphometry. Another limitation is the lack of data on trabecular bone strength, as the three-point bending test used in this study mainly reflects cortical strength. In this
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group. In rosiglitazone- treated mice the trabeculae were thinner and an increase in trabecular separation was observed. In parallel, an increase in the bone marrow space was apparent. When quantified by histomorphometry, a statistically significant
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effects of programmed administration of human parathyroid hormone fragment (1–34) on bone histomorphometry and serum chemistry in rats . Endocrinology 138 4607 – 4612 doi:10.1210/en.138.11.4607 . Faloona GR Srere PA 1969
Division of Nephrology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
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.4) for 14 days at 4°C and processed in paraffin wax. Sections were stained using Goldner’s Trichrome and reacted for tartrate-resistant acid phosphatase. Bone histomorphometry was quantified using the BioQuant Osteo software (BIOQUANT Image Analysis
Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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Department of Reproductive Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico
Institute of Biomedical Research,
School of Dentistry and
School of Chemistry, Universidad Nacional Autónoma de Mexico (UNAM), Mexico City 04510, Mexico
Department of Nephrology and Mineral Metabolism, INCMNSZ, Mexico City, Mexico
National Institute of Perinatology and School of Medicine, UNAM/Hospital General de México, Montes Urales No. 800, Col. Lomas Virreyes, Mexico, D.F., C.P. 11000, Mexico City, Mexico
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effectiveness on osteoblasts function are in line with the observation that a female murine model, carrying a null mutation of the PR gene, has not apparent alterations in peak bone mass or bone histomorphometry, thus demonstrating that PR does not play a
Bone Pathology Unit, Angers University Hospital, Angers Cedex, France
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Rheumatology Department, Angers University Hospital, Angers Cedex, France
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Bone Pathology Unit, Angers University Hospital, Angers Cedex, France
Service Commun d’Imageries et d’Analyses Microscopiques, SCIAM, SFR 42-08, Université d’Angers, IRIS-IBS Institut de Biologie en Santé, CHU d’Angers, Angers Cedex, France
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.jtemb.2013.05.004 Dempster DW Compston JE Drezner MK Glorieux FH Kanis JA Malluche H Meunier PJ Ott SM Recker RR Parfitt AM 2013 Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR
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the rat. State of mineral metabolism and bone histomorphometry. European Surgical Research 29 209 –221. Sibilia V , Cocchi D, Pagani F, Lattuada N, Moro GL, Pecile A, Rubinacci AM, Muller EE & Netti C 1999 Hexarelin