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David M Golding, Daniel J Rees, Jennifer R Davies, Dinko Relkovic, Hannah V Furby, Irina A Guschina, Anna L Hopkins, Jeffrey S Davies, James L Resnick, Anthony R Isles, and Timothy Wells

. Insight into the relationship between the imprinted genes within the PWS cluster and impaired function has been gained by comparing the phenotype of mouse models bearing different PWS-associated mutations. For example, neonatal failure to thrive and early

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H Y Li, Y X Liu, L Harvey, S Shafaeizadeh, E M van der Beek, and W Han

better treatments. Continued innovation in the development of animal models of GDM with improved translational value (i.e. a model that is truly transient) is needed. Our aim was to develop a GDM mouse model optimized for future development of

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Hyun Joon Kim, Mary C Gieske, Susan Hudgins, Beob Gyun Kim, Andree Krust, Pierre Chambon, and CheMyong Ko

). Polyclonal antisera for mouse pituitary hormones (adrenocorticotropin hormone (ACTH), GH, PRL, follicle-stimulating hormone (FSH), LH, and TSH) were purchased from the National Hormone and Pituitary Program (Harbor-UCLA Medical Center, Torrance, CA, USA

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Anne-Marie O'Carroll, Gillian M Howell, Emma M Roberts, and Stephen J Lolait

). In addition, Ucn III is expressed in mouse β-cells suggesting that the CRH system may be involved in the local regulation of pancreatic endocrine secretions ( Li et al . 2003 ). Indeed, accumulating evidence suggests that the CRH family of peptides

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H. M. LLOYD, B. M. BINDON, D. R. LAMOND, and J. D. MEARES

SUMMARY

Extracts of mouse pituitary glands, prepared by homogenization and high-speed centrifugation, were separated into 16–17 fractions by preparative electrophoresis in starch gel, from which the proteins were then recovered by an electrophoretic method.

Follicle-stimulating hormone (FSH), assayed by the uterine-weight augmentation method in the hypophysectomized mouse, was confined to 2–3 adjacent portions of the gel 1 cm. wide. Luteinizing hormone (LH) assayed by the rat ovarian ascorbic-acid depletion method was recovered from three discrete regions of the gel, well separated from FSH. The most active LH fraction was of slower mobility than the FSH. Assay for 'total' gonadotrophin, using normal mouse uterine weights, disclosed active material of slow mobility, separated from the FSH fractions. A series of experiments devoted to assays of FSH separated electrophoretically from pooled mouse pituitary glands demonstrated variations in pituitary content and concentration of FSH related to sex, age, pregnancy, lactation, dietary changes and light exposure which were consistent with results of previous studies of the mouse and of the rat.

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Shubhashree Uppangala, Akshatha Daddangadi, Jeena Susan Joseph, Sujit Raj Salian, Riddhi Kirit Pandya, Guruprasad Kalthur, and Satish Kumar Adiga

recently reported ( Kieffer et al. 2020 ). Though, mouse preimplantation embryos were found to be insensitive to the corticosterone treatment in vitro , the blastocyst development was hindered when embryos were co-cultured with oviductal epithelial cells

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I Stoykov, B Zandieh-Doulabi, A F M Moorman, V Christoffels, W M Wiersinga, and O Bakker

out to study by immunohistochemistry the distribution of TRα1 and TRβ1 in mouse heart in the adult and in different stages of the embryonic development. Within the heart, two types of myocardial components are distinguished functionally: the

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G. H. ZEILMAKER

Several studies have indicated that mouse blastocysts proliferate when transplanted to extra-uterine sites such as the eye (Runner, 1947; Fawcett, Wislocki & Waldo, 1947), kidney (Fawcett, 1950; Kirby, 1960), spleen (Kirby, 1963a) and testis (Kirby, 1963b) and that the growth of trophoblast cells is independent of the endocrine status of the host mouse.

In a normal pregnancy the trophoblast contributes to the endocrine milieu of the mouse by producing a luteotrophic substance (Newton & Beck, 1939; Cerruti & Lyons, 1960). According to Kirby (1965, 1966) this substance is produced by the decidua and, in experimental conditions, by the uterine stroma under the influence of adjacent trophoblast cells. This conclusion was borne out by his experiments in which trophoblast tissue growing under the kidney capsule would not stop the oestrous cycle, inhibit mating with ensuing pregnancy, or stimulate the corpora lutea. Moreover, a considerable degree of mammary gland stimulation

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Kuladip Jana, Xiangling Yin, Randolph B Schiffer, Jau-Jiin Chen, Akhilesh K Pandey, Douglas M Stocco, Paula Grammas, and XingJia Wang

study were obtained from either Sigma or Fisher Chemicals (Pittsburgh, PA, USA). MA-10 cell culture The MA-10 mouse Leydig tumor cell line was a generous gift from Dr Mario Ascoli (Department of Pharmacology, University of Iowa, College of Medicine, Iowa

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O A Sukocheva and D O Carpenter

), forskolin, amiodarone, KT-5720 (PKA inhibitor), cycloheximide, staurosporine (StSp), and digitonin were purchased from Sigma. Mouse TNFα and anti-Fas antibodies (clone Jo2) were purchased from Pharmingen (San Diego, CA, USA). Fas receptor, phospho-ERK (pERK