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Bernard Freudenthal Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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John Logan Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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Sanger Institute Mouse Pipelines Mouse Pipelines, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

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Peter I Croucher Garvan Institute of Medical Research, Sydney, New South Wales, Australia

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Graham R Williams Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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J H Duncan Bassett Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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evidence of variations in skeletal cellular function. Histomorphometry is performed by a recently innovated high-throughput process that involves computer-automated signal detection for the particular cell type-specific stains. Data are accrued by automated

Article Type:
Review Article
DOI:
https://doi.org/10.1530/JOE-16-0258
Volume/Issue:
Volume 231: Issue 1
Open access
Chandrika D Mahalingam Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Tanuka Datta Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Rashmi V Patil Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Jaclynn Kreider Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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R Daniel Bonfil Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Keith L Kirkwood Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Steven A Goldstein Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Abdul B Abou-Samra Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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Nabanita S Datta Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Division of Endocrinology, Department of Orthopedic Surgery, Department of Urology and Pathology, Departments of Craniofacial Biology and Microbiology and Immunology, Barbara Ann Karmanos Cancer Institute, Cardiovascular Research Institute, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201, USA

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-sectional area, BMC, and TMD were calculated after applying a uniform threshold of 2000 HU. Staining and bone histomorphometry Femora were fixed with 4% paraformaldehyde for 24 h, decalcified with 10% EDTA (pH 6.5) in PBS, dehydrated, infiltrated, and paraffin

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-11-0144
Volume/Issue:
Volume 211: Issue 2
Free access
Iris Boraschi-Diaz Shriners Hospital for Children-Canada, Montreal, Quebec, Canada
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada

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Josephine T Tauer Shriners Hospital for Children-Canada, Montreal, Quebec, Canada

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Omar El-Rifai Unité de Recherche en Physiologie Intégrative et Moléculaire, Institut de Recherches Cliniques de Montréal, Montréal, Québec, Canada

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Delphine Guillemette Shriners Hospital for Children-Canada, Montreal, Quebec, Canada
Département de Mathématiques, Université du Québec à Montréal, Montréal, Québec, Canada

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Geneviève Lefebvre Département de Mathématiques, Université du Québec à Montréal, Montréal, Québec, Canada

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Frank Rauch Shriners Hospital for Children-Canada, Montreal, Quebec, Canada

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Mathieu Ferron Unité de Recherche en Physiologie Intégrative et Moléculaire, Institut de Recherches Cliniques de Montréal, Montréal, Québec, Canada
Départements de Médecine et de Biochimie et Médecine Moléculaire, Université de Montréal, Montréal, Québec, Canada

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Svetlana V Komarova Shriners Hospital for Children-Canada, Montreal, Quebec, Canada
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada

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osteogenesis imperfecta type I . Journal of Musculoskeletal and Neuronal Interactions 14 155 – 161 . Rauch F Travers R Parfitt AM Glorieux FH 2000 Static and dynamic bone histomorphometry in children with osteogenesis imperfecta

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-17-0335
Volume/Issue:
Volume 234: Issue 3
Free access
Astrid Chamson-Reig Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of

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Edith J Arany Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of
Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of

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Kelly Summers Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of
Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of

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David J Hill Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of
Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of
Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of
Lawson Health Research Institute, Medicine, Microbiology and Immunology, Paediatrics, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of

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–15 animals derived from five separate litters of animals. * P <0.05 versus BALC/c mice of the same gender. When islet histomorphometry was analyzed at 8 weeks of age, LP-fed NOD female mice had a significantly lower number of islets per unit area of pancreas

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-09-0002
Volume/Issue:
Volume 201: Issue 2
Free access
Alessandra Bitto
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Francesca Polito
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Bruce Burnett Section of Pharmacology, Primus Pharmaceuticals, Section of Physiology and Human Nutrition, Department of Clinical and Experimental Medicine and Pharmacology, Torre Biologica 5th Floor, c/o AOU Policlinico G. Martino, Via C. Valeria Gazzi, 98125 Messina, Italy

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Robert Levy Section of Pharmacology, Primus Pharmaceuticals, Section of Physiology and Human Nutrition, Department of Clinical and Experimental Medicine and Pharmacology, Torre Biologica 5th Floor, c/o AOU Policlinico G. Martino, Via C. Valeria Gazzi, 98125 Messina, Italy

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Vincenzo Di Stefano
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Mary Ann Armbruster Section of Pharmacology, Primus Pharmaceuticals, Section of Physiology and Human Nutrition, Department of Clinical and Experimental Medicine and Pharmacology, Torre Biologica 5th Floor, c/o AOU Policlinico G. Martino, Via C. Valeria Gazzi, 98125 Messina, Italy

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Herbert Marini Section of Pharmacology, Primus Pharmaceuticals, Section of Physiology and Human Nutrition, Department of Clinical and Experimental Medicine and Pharmacology, Torre Biologica 5th Floor, c/o AOU Policlinico G. Martino, Via C. Valeria Gazzi, 98125 Messina, Italy

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Letteria Minutoli
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Domenica Altavilla
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Francesco Squadrito
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Melsen F 1994 Bone Histomorphometry . New York : Raven Press . Ettinger B Pressman A Schein J 1998 Clinic visits and hospital admissions for care of acid-related upper gastrointestinal disorders in women using alendronate for

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-08-0552
Volume/Issue:
Volume 201: Issue 3
Free access
Andrei S Chagin Pediatric Endocrinology Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Stockholm SE-17176, Sweden

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Elham Karimian Pediatric Endocrinology Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Stockholm SE-17176, Sweden

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Katja Sundström Pediatric Endocrinology Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Stockholm SE-17176, Sweden

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Emma Eriksson Pediatric Endocrinology Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Stockholm SE-17176, Sweden

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Lars Sävendahl Pediatric Endocrinology Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Stockholm SE-17176, Sweden

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flat chondrocytes (B), the hypertropic zone (D) and the size of hypertropic chondrocytes (E) were determined by histomorphometry. Number of hypertropic chondrocytes was counted manually (F). Data represent means± s.e.m . * P <0.05, ** P <0.01, and *** P

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-09-0307
Volume/Issue:
Volume 204: Issue 1
Free access
Dimitrios Agas School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Guilherme Gusmão Silva Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

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Fulvio Laus School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Andrea Marchegiani School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Melania Capitani School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Cecilia Vullo School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Giuseppe Catone School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Giovanna Lacava School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Antonio Concetti School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Luigi Marchetti School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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Maria Giovanna Sabbieti School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy

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:10.1002/jbmr.350 ) Egan KP Brennan TA Pignolo RJ 2012 Bone histomorphometry using free and commonly available software . Histopathology 61 1168 – 1173 . ( doi:10.1111/j.1365-2559.2012.04333.x ) Ehninger A Trumpp A 2011

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-16-0538
Volume/Issue:
Volume 232: Issue 2
Free access
Russell T Turner Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA
Center for Healthy Aging Research, Oregon State University, Corvallis, Oregon, USA

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Adam J Branscum Biostatistics Program, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA

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Carmen P Wong Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA

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Urszula T Iwaniec Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA
Center for Healthy Aging Research, Oregon State University, Corvallis, Oregon, USA

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Emily Morey-Holton Life Science Division, NASA Ames Research Center, Moffett Field, California, USA

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.1152/jappl.2001.90.4.1359 ) Cavolina JM Evans GL Harris SA Zhang M Westerlind KC Turner RT 1997 The effects of orbital spaceflight on bone histomorphometry and messenger ribonucleic acid levels for bone matrix proteins and skeletal signaling

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-20-0393
Volume/Issue:
Volume 247: Issue 3
Restricted access
F A Hills
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M G Elder
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T Chard
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M H F Sullivan
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. Surani MA , Barton SC & Norris ML 1987 Influence of parental chromosomes on spatial pathology in androgenetic–parthenogenetic chimaeras in the mouse. Nature 326 395 –397. Teasdale F 1989 Histomorphometry of the

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.1.05867
Volume/Issue:
Volume 183: Issue 3
Free access
Christianne M A Reijnders Departments of Endocrinology and
Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, brug 124, PO Box 7057, 1081 HV Amsterdam, The Netherlands

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Nathalie Bravenboer Departments of Endocrinology and
Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, brug 124, PO Box 7057, 1081 HV Amsterdam, The Netherlands

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Annechien M Tromp Departments of Endocrinology and
Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, brug 124, PO Box 7057, 1081 HV Amsterdam, The Netherlands

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Marinus A Blankenstein Departments of Endocrinology and
Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, brug 124, PO Box 7057, 1081 HV Amsterdam, The Netherlands

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Paul Lips Departments of Endocrinology and
Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, brug 124, PO Box 7057, 1081 HV Amsterdam, The Netherlands

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sawing microtome (FMTA, Amsterdam, The Netherlands) through the region of applied loading or through the corresponding region of the contra-lateral tibia. The endosteal surfaces of all sections were used for histomorphometry measurements. Mineralizing

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.1.06880
Volume/Issue:
Volume 192: Issue 1
Free access