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Noelia Martínez-Sánchez Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain

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José M Moreno-Navarrete CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain
Department of Diabetes, Endocrinology and Nutrition, Hospital de Girona ‘Dr Josep Trueta’, Institut D’investigació Biomèdica de Girona (IdIBGi) and University of Girona, Girona, Spain

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Cristina Contreras Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain

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Eva Rial-Pensado Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain

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Johan Fernø Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
Department of Clinical Science, KG Jebsen Center for Diabetes Research, University of Bergen, Bergen, Norway

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Rubén Nogueiras Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain

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Carlos Diéguez Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain

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José-Manuel Fernández-Real CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain
Department of Diabetes, Endocrinology and Nutrition, Hospital de Girona ‘Dr Josep Trueta’, Institut D’investigació Biomèdica de Girona (IdIBGi) and University of Girona, Girona, Spain

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Miguel López Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Santiago de Compostela, Spain

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cAMP-mediated acute rise in ucp1 gene expression ( Bianco et al . 1988 , Silva 2006 , Ribeiro et al . 2010 ). The existence of central effects of THs in the regulation of BAT thermogenesis was proposed long time ago ( Nedergaard et al . 1997

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-16-0425
Volume/Issue:
Volume 232: Issue 2
Open access
Marta Lantero Rodriguez Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Maaike Schilperoort Department of Medicine, Division of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Inger Johansson Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Elin Svedlund Eriksson Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Vilborg Palsdottir Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Jan Kroon Department of Medicine, Division of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Marcus Henricsson Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Sander Kooijman Department of Medicine, Division of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Mia Ericson Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Jan Borén Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Claes Ohlsson Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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John-Olov Jansson Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Malin C Levin Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Patrick C N Rensen Department of Medicine, Division of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Åsa Tivesten Wallenberg Laboratory for Cardiovascular and Metabolic Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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known about the potential role of androgens in the regulation of BAT activity and existing data are conflicting. Castration has been shown to decrease BAT weight ( Movérare-Skrtic et al. 2006 ) and increase BAT Ucp1 mRNA expression ( Hashimoto et al

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-20-0263
Volume/Issue:
Volume 251: Issue 1
Restricted access
Asuka Mano-Otagiri Department of Physiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan

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Hisayuki Ohata Department of Physiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan

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Azusa Iwasaki-Sekino Department of Physiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan

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Takahiro Nemoto Department of Physiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan

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Tamotsu Shibasaki Department of Physiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan

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BAT To examine the effect of ghrelin on uncoupling protein (Ucp) 1 mRNA expression in BAT, rats were killed by decapitation 3 or 6 h after i.c.v. or i.v. administration of sample and BAT was removed and stored at −80 °C. Total RNA was extracted from

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-08-0374
Volume/Issue:
Volume 201: Issue 3
Free access
Koichiro Taguchi Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Kazuo Kajita Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Yoshihiko Kitada Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Masayuki Fuwa Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Motochika Asano Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Takahide Ikeda Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Toshiko Kajita Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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Tatsuo Ishizuka Department of General Internal Medicine and Rheumatology, Gifu Municipal Hospital, Gifu, Japan

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Itaru Kojima Laboratory of Cell Physiology, Institute for Molecular and Cellular Regulation, Gunma University, Gunma, Japan

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Hiroyuki Morita Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu, Japan

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beige cells, in white adipose tissue ( Vitali et al . 2012 , Chen et al. 2013 ). Uncoupling protein 1 (UCP1) is a major marker of beige cells, as well as brown adipose tissue (BAT). Despite numerous similarities, however, BAT and beige cells differ

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-19-0503
Volume/Issue:
Volume 245: Issue 1
Open access
Riccardo Dore Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Luka Levata Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Sogol Gachkar Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Olaf Jöhren Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Jens Mittag Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Hendrik Lehnert Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Carla Schulz Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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, Hwa et al. 2001 ) by enhancing sympathetic nerve activity, uncoupling protein 1 ( Ucp1 ) mRNA expression and temperature in interscapular brown adipose tissue (iBAT) ( Williams et al. 2003 , Fan et al. 2007 , Song et al. 2008 ). The

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-17-0151
Volume/Issue:
Volume 235: Issue 2
Free access
Anna G Holmes Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Jose L Mesa Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Bronwyn A Neill Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia
Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Jason Chung Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Andrew L Carey Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Gregory R Steinberg Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Bruce E Kemp Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia
Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Robert J Southgate Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Graeme I Lancaster Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Clinton R Bruce Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Matthew J Watt Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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Mark A Febbraio Diabetes and Metabolism Division, School of Medical Sciences, St Vincent's Institute and Department of Medicine, CSIRO Molecular and Health Technologies, Department of Physiology, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia

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treatment ( Fig. 5 B), UCP2 expression was elevated in both phasic and chronic IL-6 treatment ( Fig. 5 B). Figure 4 AMPK α1 and α2 activity in (A) EDL muscle and (B) liver from rats treated for 14 d with vehicle control (control) or 2.4 μg/day interleukin-6

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-08-0113
Volume/Issue:
Volume 198: Issue 2
Free access
J C Sousa Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus Gualtar, Braga, Portugal
Institute for Molecular and Cell Biology, Rua do Campo Alegre, Porto, Portugal
ICBAS, University of Porto, Largo Professor Abel Salazar, Porto, Portugal
Molecular Endocrinology Unit, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC & UAM, Madrid, Spain
Department of Production & Systems Engineering, University of Minho, Campus Gualtar, Braga, Portugal

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G Morreale de Escobar Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus Gualtar, Braga, Portugal
Institute for Molecular and Cell Biology, Rua do Campo Alegre, Porto, Portugal
ICBAS, University of Porto, Largo Professor Abel Salazar, Porto, Portugal
Molecular Endocrinology Unit, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC & UAM, Madrid, Spain
Department of Production & Systems Engineering, University of Minho, Campus Gualtar, Braga, Portugal

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P Oliveira Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus Gualtar, Braga, Portugal
Institute for Molecular and Cell Biology, Rua do Campo Alegre, Porto, Portugal
ICBAS, University of Porto, Largo Professor Abel Salazar, Porto, Portugal
Molecular Endocrinology Unit, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC & UAM, Madrid, Spain
Department of Production & Systems Engineering, University of Minho, Campus Gualtar, Braga, Portugal

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M J Saraiva Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus Gualtar, Braga, Portugal
Institute for Molecular and Cell Biology, Rua do Campo Alegre, Porto, Portugal
ICBAS, University of Porto, Largo Professor Abel Salazar, Porto, Portugal
Molecular Endocrinology Unit, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC & UAM, Madrid, Spain
Department of Production & Systems Engineering, University of Minho, Campus Gualtar, Braga, Portugal

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J A Palha Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus Gualtar, Braga, Portugal
Institute for Molecular and Cell Biology, Rua do Campo Alegre, Porto, Portugal
ICBAS, University of Porto, Largo Professor Abel Salazar, Porto, Portugal
Molecular Endocrinology Unit, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC & UAM, Madrid, Spain
Department of Production & Systems Engineering, University of Minho, Campus Gualtar, Braga, Portugal

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genes including those of uncoupling protein 1 (UCP1) ( Bouillaud et al. 1984 , Silva & Rabelo 1997 ) and deiodinase type 2 (DII) ( Silva & Larsen 1983 , Klingenspor 2003 ). Augmented T 3 resulting from increased DII activity also contributes to

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.1.06406
Volume/Issue:
Volume 187: Issue 2
Free access
Chan-Juan Ma Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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Ai-Fang Nie Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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Zhi-Jian Zhang Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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Zhi-Guo Zhang Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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Li Du Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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Xiao-Ying Li Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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Guang Ning Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China
Shanghai Key Laboratory of Endocrine Tumor, Division of Endocrinology and Metabolism, Shanghai Jiao-Tong University School of Medicine, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, 197 Rui-Jin 2nd Road, Shanghai 200025, People's Republic of China

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hyperactivation of c-Jun N-terminal kinase (JNK) and subsequent serine phosphorylation of insulin receptor substrate-1 (IRS-1), thus impairing insulin-stimulated glucose uptake in 3T3-L1 adipocytes. With these contradictory reports, the role of genipin in insulin

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-11-0473
Volume/Issue:
Volume 216: Issue 3
Free access
Li Zhao Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Chunfang Zhu Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Meng Lu Research Center for Clinical Medicine, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Chi Chen Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Xiaomin Nie Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Buatikamu Abudukerimu Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Kun Zhang Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Zhiyuan Ning Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Yi Chen Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Jing Cheng Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Fangzhen Xia Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Ningjian Wang Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Michael D Jensen Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA

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Yingli Lu Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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), forward 5′-TTC GGC AAA GGC CTG ATC A-3′ and reverse 5′-TTG CCT TTG TCC CGG AAA TG-3′; uncoupling protein-1 ( Ucp1 ), forward 5′-ATC AAA CCC CGC TAC ACT GG-3′ and reverse 5′-CAG TAA ATG GCA GGG GAC GT-3′; peroxisome proliferator activated receptor gamma

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DOI:
https://doi.org/10.1530/JOE-18-0374
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Volume 240: Issue 2
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Daiana Araujo Santana-Oliveira Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil

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Henrique Souza-Tavares Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil

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Aline Fernandes-da-Silva Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil

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Flavia Maria Silva-Veiga Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil

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Gustavo Casimiro-Lopes Department of Gymnastics, Physical Education and Sports Institute, Laboratory of Exercise Pathophysiology (LAFE), Rio de Janeiro State University, Rio de Janeiro, Brazil

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Patricia Cristina Lisboa Laboratory of Endocrine Physiology, Biology Institute, Rio de Janeiro State University, Rio de Janeiro, Rio de Janeiro, Brazil

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Carlos Alberto Mandarim-de-Lacerda Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil

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Vanessa Souza-Mello Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil

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-1473, 1:100), or UCP1 (Cusabio, PA025554ESR2HU, Houston, TX, USA, 1:150) for 2 h at room temperature, iBAT slides. The antibodies were diluted in 1.5% horse serum (Vector Laboratories, CA, USA). Afterward, the slides were incubated with pan

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DOI:
https://doi.org/10.1530/JOE-23-0123
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Volume 259: Issue 1
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