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Ziping Jiang Department of Hand and Foot Surgery, The First Hospital of Jilin University, Changchun, Jilin, China

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Junduo Wu Department of Cardiology, The Second Hospital of Jilin University

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Fuzhe Ma Department of Nephrology, The First Hospital of Jilin University, Changchun, Jilin, China

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Jun Jiang Department of Neurosurgery, The Second Hospital of Shandong University, Jinan, Shandong, China

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Linlin Xu Department of Neurology, The Second Hospital of Shandong University, Jinan, Shandong, China

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Lei Du Department of Nutrition and Food Hygiene, School of Public Health, Shandong University, Jinan, Shandong, China

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Wenlin Huang School of Science and Technology, Georgia Gwinnett College, Lawrenceville, Georgia, USA

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Zhaohui Wang Department of Acupuncture and Tuina, Changchun University of Chinese Medicine, Changchun, Jilin, China

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Ye Jia Department of Diabetes Complications and Metabolism, Diabetes Metabolism Research Institute, Beckman Research Institute of City of Hope, Duarte, California, USA

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Laijin Lu Department of Hand and Foot Surgery, The First Hospital of Jilin University, Changchun, Jilin, China

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Hao Wu Department of Nutrition and Food Hygiene, School of Public Health, Shandong University, Jinan, Shandong, China

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-200a/KEAP1/NRF2 signaling in the diabetic endothelium, as well as the impact of miR-200a supplementation on ED. In the present study, we first explored the effect of high glucose (HG) on aortic endothelial cells (ECs), showing that HG altered miR-200

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Morag J Young Cardiovascular Endocrinology, Department of Physiology, MIMR-PHI Institute, 27–31 Wright St, Clayton 3168, Australia
Cardiovascular Endocrinology, Department of Physiology, MIMR-PHI Institute, 27–31 Wright St, Clayton 3168, Australia

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Amanda J Rickard Cardiovascular Endocrinology, Department of Physiology, MIMR-PHI Institute, 27–31 Wright St, Clayton 3168, Australia
Cardiovascular Endocrinology, Department of Physiology, MIMR-PHI Institute, 27–31 Wright St, Clayton 3168, Australia

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numerous pathological conditions including atherosclerosis, inflammation and tissue remodelling. The endothelium expresses MR, GR and HSD2 (HSD11B2) all of which drive numerous physiological and pathological functions in the vascular wall. Clinical and

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Marta Toral Departamento de Farmacología, Facultad de Farmacia, Granada, Spain

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Rosario Jimenez Departamento de Farmacología, Facultad de Farmacia, Granada, Spain
Instituto de Investigación Biosanitaria GRANADA, Hospitales Universitarios de Granada, Universidad de Granada, Granada, Spain

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Sebastián Montoro-Molina Departamento de Ciencias de la Salud, Universidad de Jaén, Jaén, Spain

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Miguel Romero Departamento de Farmacología, Facultad de Farmacia, Granada, Spain
Instituto de Investigación Biosanitaria GRANADA, Hospitales Universitarios de Granada, Universidad de Granada, Granada, Spain

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Rosemary Wangensteen Departamento de Ciencias de la Salud, Universidad de Jaén, Jaén, Spain

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Juan Duarte Departamento de Farmacología, Facultad de Farmacia, Granada, Spain
Instituto de Investigación Biosanitaria GRANADA, Hospitales Universitarios de Granada, Universidad de Granada, Granada, Spain

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Félix Vargas Instituto de Investigación Biosanitaria GRANADA, Hospitales Universitarios de Granada, Universidad de Granada, Granada, Spain
Departamento de Fisiología, Facultad de Medicina, Granada, Spain

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be rate limiting for NO synthesis. In addition, human umbilical vein endothelium also requires the activity of the cationic and neutral amino acid transport system y + L for NO synthesis ( Arancibia-Garavilla et al. 2003 ). After observing that L

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Yunshan Hu Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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Hong Bin Liu Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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Richard W Simpson Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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Anthony E Dear Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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( Bar-Tana 2001 ). PPARγ is expressed most abundantly in adipose tissue, pancreatic β cells, vascular endothelium and macrophages ( Dubois et al . 2000 , Willson et al . 2001 ). Some evidence suggests that the effects of TZDs are mediated independent

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Ian M Bird Perinatal Research Laboratories, Department of Obstetrics and Gynecology, University of Wisconsin–Madison, 7E Meriter Hospital/Park, 202 South Park Street, Madison, Wisconsin 53715, USA

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. References Boeldt DS Yi FX Bird IM 2011 Pregnancy adaptive programming of capacitative entry responses alters nitric oxide (NO) output in vascular endothelium: new insights into eNOS regulation through adaptive cell signaling . Journal of

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Rong Wan Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Chao Zhu Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Rui Guo Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Lai Jin Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Yunxin Liu Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Li Li Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Hao Zhang Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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Shengnan Li Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, People's Republic of China

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affinity than testosterone and 15- to 30-fold greater affinity than adrenal androgens for androgen receptor (AR; Ikeda et al . 2012 ). Reportedly, DHT enhanced the binding of monocytes to the endothelium via increased expression of vascular cell adhesion

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Hong-Yo Kang Graduate Institute of Clinical Medical Sciences, Hormone Research Center, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan

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testosterone on vascular reactivity and inflammation. The majority of animal studies support the hypothesis that the actions of testosterone on vascular relaxation are both endothelium-dependent and -independent vasodilatory effects. Endothelial

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Félix Vargas
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Isabel Rodríguez-Gómez
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Pablo Vargas-Tendero
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Eugenio Jimenez Departamento de Fisiología, Departamento de Bioquímica y Biología Molecular e Inmunología, Facultad de Medicina, Universidad de Granada, E-18012 Granada, Spain

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Mercedes Montiel Departamento de Fisiología, Departamento de Bioquímica y Biología Molecular e Inmunología, Facultad de Medicina, Universidad de Granada, E-18012 Granada, Spain

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system, by removing the C-terminal histidyl-leucine residue from AI. ACE is mainly located in the pulmonary vascular endothelium but has also been found in kidney, liver, brain, and cardiovascular tissues, among others. ACE activity is influenced by

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Claire U Onyimba Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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Neelima Vijapurapu Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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S John Curnow Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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Pamela Khosla Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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Paul M Stewart Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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Philip I Murray Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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Elizabeth A Walker Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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Saaeha Rauz Academic Unit of Ophthalmology, Division of Immunity and Infection and
Department of Endocrinology, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 5TT, UK

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and immune regulation that are vital for sight. Not only MR and GR, but also 11β-HSD1, are expressed throughout the human eye (MR: corneal epithelium, endothelium, iris, non-pigmented epithelium (NPE), pigmented epithelium (PE), ciliary body and

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Laura L Hernandez Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA
Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA

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Sean W Limesand Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA

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Jayne L Collier Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA

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Nelson D Horseman Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA

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Robert J Collier Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA

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myoepithelial cells, and asteriks (*) correspond to vascular endothelium. Expression of HTR1B , 2A , 2B , 4 , and 7 was each localized in BMT ( Fig. 2 , row A, columns 1–5). Counterstaining with GS-I (vascular endothelium) indicated that HTR1B , 2A , and

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