.5, where I o is the fasting insulin and G o is the fasting glucose, as described by Matthews et al . (1985) . Circulating leptin and adiponectin levels were measured with a coated-tube IRMA kit (Diagnostic Systems Laboratories, Sinsheim, Germany) and by
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Bee K Tan, Jing Chen, Raghu Adya, Manjunath Ramanjaneya, Vanlata Patel, and Harpal S Randeva
Maayan Vatarescu, Sapir Bechor, Yulia Haim, Tal Pecht, Tanya Tarnovscki, Noa Slutsky, Ori Nov, Hagit Shapiro, Avishai Shemesh, Angel Porgador, Nava Bashan, and Assaf Rudich
, revealing highly diverse response of these secreted products in adipose tissue of HFF→NC compared to HFF ( Fig. 4C ). Among others, concentrations of leptin and RBP4 were not different in HFF→NC compared to HFF, adiponectin and IL-10, but also resistin
A Feraco, A Armani, R Urbanet, A Nguyen Dinh Cat, V Marzolla, F Jaisser, and M Caprio
-Lox approach: mice expressing tamoxifen-inducible Cre recombinase under the control of the adiponectin (Adipo-) promoter (kindly provided by S Offermans, Heidelberg, GE) ( Sassmann et al. 2010 ) were crossed with mice containing Nr3c2 -flox alleles (kindly
Sandra Pereira, Wen Qin Yu, María E Frigolet, Jacqueline L Beaudry, Yaniv Shpilberg, Edward Park, Cristina Dirlea, B L Grégoire Nyomba, Michael C Riddell, I George Fantus, and Adria Giacca
). Plasma FFA concentrations were determined using a colorimetric assay (Wako Pure Chemical Industries, Ltd, Osaka, Japan) ( Park et al . 2007 ). Plasma adiponectin concentrations during the basal period were determined using a kit (Cat # EZRADP-62K, EMD
Yun Wang, Yue Zheng, Patsy M Nishina, and Jürgen K Naggert
., Diagnostic Division, Brea, CA, USA). The plasma hormone levels of leptin, adiponectin, resistin, and insulin were measured by ELISA (Linco Research, St Charles, MO, USA). For the glucose tolerance test, mice were fasted for 16 h, and were given i.p. injection
Eriko Inoue, Toshihiro Ichiki, Kotaro Takeda, Hirohide Matsuura, Toru Hashimoto, Jiro Ikeda, Aya Kamiharaguchi, and Kenji Sunagawa
′-GCTTCTTTGGGACACCTGCTGC-3′. PPARγ (PPARG): 5′-TGTCGGTTTCAGAAGTGCCTTG-3′; 5′-TTCAGCTGGTCGATATCACTGGAG-3′. C/EBPα: 5′-TTGAAGCACAATCGATCCATCC-3′; 5′-GCACACTGCCATTGCACAAG-3′. Adiponectin: 5′-GTCAGTGGATCTGACGACACCAA-3′; 5′-ATGCCTGCCATCCAACCTG-3′. 18S rRNA: 5
Ruben Rodriguez, Jacqueline N Minas, Jose Pablo Vazquez-Medina, Daisuke Nakano, David G Parkes, Akira Nishiyama, and Rudy M Ortiz
within-subjects factor. a P < 0.05 vs LETO; b P < 0.05 vs OLETF; # P < 0.05 vs baseline. Chronic AT 1 blockade decreases fasting plasma glucose FPG, TG, NEFA, adiponectin and IRI were measured to assess whether the timing of AT
Ali Aflatounian, Melissa C Edwards, Valentina Rodriguez Paris, Michael J Bertoldo, Reena Desai, Robert B Gilchrist, William L Ledger, David J Handelsman, and Kirsty A Walters
three sections of the fat pad, with at least 200 µm separating these sections, as described ( Caldwell et al. 2014 ). Adiponectin assay Total full-length mouse adiponectin concentrations were measured in serum using a Quantikine ELISA Kit from
Stephanie L Clookey, Rebecca J Welly, Terese M Zidon, Michelle L Gastecki, Makenzie L Woodford, Zachary I Grunewald, Nathan C Winn, Dusti Eaton, Natalia G Karasseva, Harold S Sacks, Jaume Padilla, and Victoria J Vieira-Potter
)) and an index of adipose tissue insulin resistance (ADIPO-IR) was calculated as the product of fasting insulin (μU/L) and fasting NEFAs (mmol/L) ( Lomonaco et al. 2012 ). Fasting levels of circulating adipokines, leptin and adiponectin, were measured
Joëlle Dupont, Sophie Tesseraud, Michel Derouet, Anne Collin, Nicole Rideau, Sabine Crochet, Estelle Godet, Estelle Cailleau-Audouin, Sonia Métayer-Coustard, Michel J Duclos, Christian Gespach, Tom E Porter, Larry A Cogburn, and Jean Simon
-Deiodinase GGAAATCGTGTCTGATGTTGTCA AACCCCCCCCATAAATAGGA IGFBP1 CTATTTGCCCAACTGTAACAAG CAGCACCCAGCGGAATCT Adiponectin GCCAGGTCTACAAGGTGTCA CCATGTGTCCTGGAAATCCT Adiponectin R1 CCAGGAGAAGGTTGTGTTTG TGATCAGCAGTGCAATTCCT Adiponectin R2 TCATGGCTCTTCCACACAGT