Search Results
Academy of Scientific and Innovative Research (AcSIR), CSIR-CDRI Campus, Lucknow, India
Search for other papers by Vinay Shukla in
Google Scholar
PubMed
Academy of Scientific and Innovative Research (AcSIR), CSIR-CDRI Campus, Lucknow, India
Search for other papers by Jyoti Bala Kaushal in
Google Scholar
PubMed
Search for other papers by Pushplata Sankhwar in
Google Scholar
PubMed
Search for other papers by Murli Manohar in
Google Scholar
PubMed
Academy of Scientific and Innovative Research (AcSIR), CSIR-CDRI Campus, Lucknow, India
Search for other papers by Anila Dwivedi in
Google Scholar
PubMed
, and the inhibition of β-catenin activation results in the loss of the differentiation potential of hESCs ( Herington et al . 2007 , Patterson et al. 2017 ). In human endometrium, NF-kB levels are typically elevated during the premenstrual phase and
Search for other papers by Delphine Benaitreau in
Google Scholar
PubMed
Search for other papers by Esther Dos Santos in
Google Scholar
PubMed
Search for other papers by Marie-Christine Leneveu in
Google Scholar
PubMed
Search for other papers by Nadia Alfaidy in
Google Scholar
PubMed
Search for other papers by Jean-Jacques Feige in
Google Scholar
PubMed
Search for other papers by Philippe de Mazancourt in
Google Scholar
PubMed
Search for other papers by René Pecquery in
Google Scholar
PubMed
Search for other papers by Marie-Noëlle Dieudonné in
Google Scholar
PubMed
Introduction Implantation requires a number of distinct cellular functions including attachment and invasion of the extravillous trophoblasts (EVTs) into the endometrium. Deficient trophoblastic invasion contributes to the poor success rates of IVF
Search for other papers by H. C. CECIL in
Google Scholar
PubMed
Search for other papers by J. BITMAN in
Google Scholar
PubMed
Search for other papers by M. R. CONNOLLY in
Google Scholar
PubMed
Search for other papers by T. R. WRENN in
Google Scholar
PubMed
SUMMARY
Glycogen was investigated in uteri of intact and progesterone-treated spayed rats with and without deciduomata. Samples of whole uterus, endometrium and myometrium were analysed. With development of deciduoma in intact animals the glycogen concentration of whole uterus increased from 68 to 125 mg./100 g. wet weight. There was no change in the myometrial glycogen concentration; i.e. 74 mg./100 g. without deciduoma and 73 mg./100 g. wet weight in the decidual myometrium. The endometrial glycogen content of decidual tissue was 221 mg./100 g. wet weight. Since myometrial glycogen was constant, the increases observed in the decidual tissue of whole uteri must be due to an increase in the amount of endometrium and/or an increase in the concentration of glycogen in the endometrium. As the deciduoma developed the proportion of endometrium increased from 9% in the uninjured horn to 34% in the injured horn. Thus, an increase in the amount of endometrium contributes to the increase in the glycogen concentration. Similar changes were observed in whole uterus, myometrium and endometrium of the spayed animals treated with progesterone. Previous work on uterine glycogen in rats indicated that oestrogens cause glycogen deposition and this occurs only in the myometrium, while progesterone exhibits no effect. The present results demonstrate that progesterone is responsible for the glycogen increase by stimulating the growth of endometrium—a glycogen-rich tissue. Since no endometrial tissue could be obtained from horns without decidual development, this study could not determine whether progesterone had any effect on glycogen deposition.
Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
Search for other papers by Hyungchul Han in
Google Scholar
PubMed
Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
Search for other papers by Kathleen J Austin in
Google Scholar
PubMed
Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
Search for other papers by Lea A Rempel in
Google Scholar
PubMed
Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
Search for other papers by Thomas R Hansen in
Google Scholar
PubMed
uterus. Interferon (IFN)-τ is a major paracrine signal that is produced by the bovine conceptus and acts on the endometrium to elicit secondary responses that are necessary to maintain pregnancy ( Roberts et al. 1992 , Thatcher et al. 1995 , Spencer
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Yan Su in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Sujuan Guo in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Chunyan Liu in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Na Li in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Shuang Zhang in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Yubin Ding in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Xuemei Chen in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Junlin He in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Xueqing Liu in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Yingxiong Wang in
Google Scholar
PubMed
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China
Search for other papers by Rufei Gao in
Google Scholar
PubMed
. 2019 ). It is a synchronized molecular dialogue between embryo and maternal endometrium, which is conducted via cytokines as well as by the expression of corresponding receptors ( Hess et al . 2007 , Boeddeker et al . 2015 ). During the early stage
Search for other papers by BENITA S. KATZENELLENBOGEN in
Google Scholar
PubMed
Search for other papers by R. E. LEAKE in
Google Scholar
PubMed
SUMMARY
Using two methods for preparation of endometrial and myometrial tissue fractions – a new method based on mincing and gentle maceration of the immature rat uterus, or separation by dissection of endometrium and myometrium from the mature uterus – both controlled histologically, we have analysed the distribution of the oestrogen-induced protein (IP), and total protein and DNA in these endometrial and myometrial fractions. Studies with both immature and mature oestrogen-stimulated uteri indicate that IP is synthesized in both the endometrial and myometrial tissues. In the immature uterus, there was a marked enrichment of IP in the endometrial fraction. When macerated for 20 or 50 s, 60–70% of the total IP, 5% of the total DNA, 9% of the total protein, and 24–30% of the total soluble protein were found in the endometrium. This enrichment is shown to be due most likely to preferential synthesis of IP in the endometrium. In contrast, in the mature uterus, there was no significant enrichment of IP in the endometrium. Since IP synthesis is associated with the action of oestrogen on both endometrium and myometrium, and the ultimate physiological effects of oestrogen on the two tissues seem to be different, the divergence of the action of oestrogen in these tissues may occur at some point after IP synthesis.
Search for other papers by J. L. Vallet in
Google Scholar
PubMed
Search for other papers by P. J. Barker in
Google Scholar
PubMed
Search for other papers by G. E. Lamming in
Google Scholar
PubMed
Search for other papers by N. Skinner in
Google Scholar
PubMed
Search for other papers by N. S. Huskisson in
Google Scholar
PubMed
ABSTRACT
Ovine trophoblast protein-1 (oTP-1), stimulates the secretion of several proteins in explant culture of day-12 cyclic ovine endometrium. We partially purified and identified one of these proteins, an 11,000 Mr, pI approx. 6 protein by N-terminal amino acid sequencing and immunoprecipitation using antibody to human β2-microglobulin. The protein was purified from cultures of endometrium collected from day-16 pregnant ewes. The N-terminal amino acid sequence was 40–55% homologous to β2-microglobulin from a variety of species. Antibody to human β2-microglobulin immunoprecipitated the protein and another protein of similar molecular weight but more acidic pi. Using immunoprecipitation of radiolabelled proteins from culture, we demonstrated that oTP-1 increased production of this protein by 40% (P<0.05). We conclude that oTP-1 increases the secretion of a β2-microglobulin-like protein from day-12 non-pregnant endometrium in culture.
Search for other papers by J. P. CHU in
Google Scholar
PubMed
Search for other papers by C. C. LEE in
Google Scholar
PubMed
Search for other papers by S. S. YOU in
Google Scholar
PubMed
Search for other papers by KUNHARD POLLOW in
Google Scholar
PubMed
Search for other papers by ELMAR BOQUOI in
Google Scholar
PubMed
Search for other papers by HORST LÜBBERT in
Google Scholar
PubMed
Search for other papers by BARBARA POLLOW in
Google Scholar
PubMed
Institut für Molekularbiologie und Biochemie, Frauenklinik Charlottenburg, Freie Universität Berlin, Germany
(Received 15 April 1975)
Recent results obtained by Tseng & Gurpide (1974) and Pollow, Lübbert, Boquoi, Kreuzer, Jeske & Pollow (1975 a) have shown that oestradiol is oxidized about ten times more rapidly by normal human endometrium of the secretory phase than by tissue of the proliferative phase. This possibly means that progesterone regulates (by way of a receptor mechanism?) the activity of the 17β-hydroxysteroid dehydrogenase (17β-HSD) which catalyses the oxidation of oestradiol in the endometrial target cell. Since progestational agents play a definite role in the management of advanced adenocarcinoma of the endometrium the present study evaluates the effect of gestagen therapy upon the 17β-HSD activity in this malignancy.
Normal endometrium (proliferative and secretory, 18 cases of each) was obtained by curettage. Endometrial adenocarcinomata (well, moderately well and undifferentiated, four cases of each) were obtained by curettage (before
Search for other papers by J. H. MARSTON in
Google Scholar
PubMed
Search for other papers by W. A. KELLY in
Google Scholar
PubMed
SUMMARY
In the rat, when luminal continuity was established between the two uterine horns, a unilateral intra-uterine device (IUD) exerted a complete, bilateral contraceptive effect. This was related to the elimination of blastocysts from both uterine horns and to the bilateral inhibition of the decidual cell reaction. Both uterine horns showed leucocytic infiltration into the endometrium, although infiltration was greater in the IUD horn than in the control horn.
When the uterine horns were united without the establishment of luminal continuity a unilateral IUD exerted a strictly unilateral effect, and leucocytic infiltration was only detected in the IUD endometrium and uterine lumen.
These results support the view that the environment within the separate or continuous uterine lumina was altered in the presence of an IUD so that the decidual cell response was inhibited and blastocysts were eliminated. The possibility that this change was related to leucocytic infiltration into the endometrium and uterine lumen cannot be eliminated.
