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(DIO) enzymes. Endogenous sex steroids, both estrogen and testosterone, play an important yet poorly defined role in regulation of the hypothalamus–pituitary–thyroid (HPT) axis and TH homeostasis. Clinical evidence demonstrated that female patients have
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SUMMARY
Isoelectric focusing in polyacrylamide gel followed by staining with Coomassie brilliant blue was used for the densitometric estimation of ovine prolactin standard and canine pituitary prolactin. The results were compared with those obtained by conventional polyacrylamide gel electrophoretic procedures and isoelectric focusing was found to be a valid technique for the estimation of prolactin and to offer greater technical convenience.
The mobility of ovine and canine prolactin was similar in isoelectric focusing and gel electrophoresis. The isoelectric point of ovine and canine prolactin was found to be 6·17 and 6·61 respectively.
Constant levels of prolactin were found in the pituitaries of bitches at oestrus.
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Introduction Sex differences in hypothalamo–pituitary–adrenal axis (HPA) activity are well documented and a number of studies have demonstrated that gonadal steroids act as regulators of HPA activity ( Viau & Meaney 1991 , Freeman
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neuroendocrine and endocrine regulation, pituitary gonadotropins are also subject to local paracrine regulation by activin ( Yam et al . 1999 a , Yuen & Ge 2004 ), similar to the situation in mammals ( Roberts et al . 1989 , Corrigan et al . 1991
Endocrinology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy
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Pituitary Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands
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Department of Internal Medicine and & Medical Specialties (DIMI) and Center of Excellence for Biomedical Research (CEBR), University of Genoa, Genoa, Italy
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Pituitary Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands
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Introduction The presence of an ACTH-secreting pituitary adenoma (corticotroph adenoma) in the anterior or intermediate lobe of the pituitary gland is the cause of Cushing’s disease (CD), a severe systemic condition characterized by a chronic
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1. Procedures are described for the approximate assessment, in the intact cat, of the diabetogenic potency of growth hormone preparations from ox anterior-pituitary glands (section A).
2. Partially depancreatized rats (three strains) and alloxan-diabetic rats (two strains) were insufficiently sensitive to such preparations to be of use as test animals (section B).
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Introduction During fasting, profound changes occur in the hypothalamic part of the hypothalamic–pituitary–thyroid axis (HPT axis), i.e. increased type 2 deiodinase (D2) activity in the arcuate nucleus (ARC) ( Diano et al. 1998
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The development of trophic hormones in foetal pituitary cells has been shown by the use of fluorescent antibodies, differential staining techniques and bioassay of pituitary homogenates. In foetal rat pituitaries, growth hormone, thyrotrophin and adrenocorticotrophin have been detected by radioimmunoassay and bioassay (Contopoulos & Simpson, 1957; Phillips & Schmidt, 1958; Milkovic & Milkovic, 1962; Birge, Peake, Mariz & Daughaday, 1967); but prolactin has not been detected by these techniques.
During experiments involving disc electrophoresis of rat pituitary homogenates the pituitaries from foetal rats were examined and a protein band appeared in the gel in a position almost identical with that of prolactin in the maternal pituitary (Plate). Rat pituitary hormones separated by disc electrophoresis according to the method of Davis (1964) have been identified by Jones, Fisher, Lewis & Vanderlaan (1965). Prolactin is the hormone that has the greatest mobility in this system; maternal prolactin had an R F value
IGH/UPR, CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 05, France
Department of Animal Physiology, Faculty of Science, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
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IGH/UPR, CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 05, France
Department of Animal Physiology, Faculty of Science, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
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IGH/UPR, CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 05, France
Department of Animal Physiology, Faculty of Science, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
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IGH/UPR, CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 05, France
Department of Animal Physiology, Faculty of Science, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
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IGH/UPR, CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 05, France
Department of Animal Physiology, Faculty of Science, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
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Introduction Endocrine control of osmoregulation has been studied only in a limited number of teleostean species, with an emphasis on salmonids. These studies underline the importance of pituitary hormones, such as prolactin ( Hirano
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Introduction Differentiation of anterior pituitary cells is thought to be controlled by various factors from the hypothalamus, peripheral hormones and/or growth factors ( Schwardz 2000 , Denef 2003 ). In fetal mice, hormone-producing cells except
