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Zhenguang Zhang, Agnes E Coutinho, Tak Yung Man, Tiina M J Kipari, Patrick W F Hadoke, Donald M Salter, Jonathan R Seckl, and Karen E Chapman

Biotec Ltd., Bisley, Surrey, UK) by magnetic-activated cell sorting as described previously ( Coutinho et al . 2016 ). Macrophages were purified by adherence to tissue culture plates using an established procedure that gives >90% purity ( Zhang et al

Open access

Pauline Campos, Jamie J Walker, and Patrice Mollard

done by hand (ROI Manager in Fiji) in very scattered populations, but for large data sets that contain a dense populations of neurons, automated cell-sorting algorithms may be better suited to extract spatial and temporal properties of individual

Open access

Louise Grahnemo, Caroline Jochems, Annica Andersson, Cecilia Engdahl, Claes Ohlsson, Ulrika Islander, and Hans Carlsten

). A Tris-buffered 0.83% (w/v) NH 4 Cl solution, pH 7.29, was used to lyse erythrocytes, and the cells were washed and re-suspended in fluorescence-activated cell sorting (FACS)-buffer (PBS supplemented with 1% (v/v) FCS and 0.1% (v/v) NaAz). Labeling

Open access

Sushil K Mahata, Hong Zheng, Sumana Mahata, Xuefei Liu, and Kaushik P Patel

site of processing, packaging and sorting of proteins and lipids en route from the ER to the plasma membrane and other destinations ( Sengupta & Linstedt 2011 ). Increased catecholamine synthesis in HF rats would require formation of more vesicles for

Open access

Koichiro Taguchi, Kazuo Kajita, Yoshihiko Kitada, Masayuki Fuwa, Motochika Asano, Takahide Ikeda, Toshiko Kajita, Tatsuo Ishizuka, Itaru Kojima, and Hiroyuki Morita

, determination of adipose progenitor cells (APC) as the initiation of differentiation and emergence of adipose-specific genes as the terminal differentiation. Studies using fluorescence-activated cell sorting and lineage tracing have characterized APC in white

Open access

Alyce M Martin, Emily W Sun, and Damien J Keating

, Martin et al . 2017 a , Lund et al . 2018 ), particularly the availability of transgenic mouse lines in which specific EE cell populations can be fluorescently labelled and sorted ( Reimann et al . 2008 ), have provided valuable insights into our