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Jane J Reavey MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Catherine Walker MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Alison A Murray MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Savita Brito-Mutunayagam MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Sheona Sweeney MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Moira Nicol MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Ana Cambursano MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Hilary O D Critchley MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Jacqueline A Maybin MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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decidualised in the high-fat diet group is clinically relevant, as this is a similar selection process to our inclusion of women with a high BMI who have regular menstrual cycles, tha is, are likely to be ovulating regularly and therefore undergo spontaneous

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Hiroyuki Enomoto Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan
Department of Neurosurgery, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Kinuyo Iwata Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Keisuke Matsumoto Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Mai Otsuka Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Akio Morita Department of Neurosurgery, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Hitoshi Ozawa Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Introduction Encoded by the Kiss1 gene, kisspeptin and its receptor, G protein-coupled receptor 54, stimulate the release of gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) in mammals and are crucial in ovulation regulation

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Nerine T Joseph Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK
Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK

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Kevin Morgan Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK

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Robin Sellar Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK

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Derek McBride Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK

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Robert P Millar Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK
Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK

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Ian C Dunn Royal (Dick) School of Veterinary Studies, MRC Human Reproductive Sciences Unit, MRC/UCT, The Roslin Institute, University of Edinburgh, Roslin, Midlothian, Edinburgh EH25 9PS, UK

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GnRH-II levels do decrease when the preovulatory release of LH is maximal or declining in the hen. Thus, GnRH-II may be involved in neuroendocrine events preceding ovulation ( Wilson et al . 1990 ). In other avian species GnRH-II affects reproductive

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