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We prepared primary hippocampal neuronal cultures from newborn (P0) Sprague–Dawley (SD) rat pup brains according to the recently published protocols ( Kaech & Banker 2006 , Beaudoin et al . 2012 ), which we have used in our previous study
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Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
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Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
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insulin and TNFα were measured using rat ultrasensitive ELISA Kits (Alpco, Laboratory Diagnostics (Caringbah, NSW, Australia) and R&D Systems, Inc. (Minneapolis, MN, USA) respectively). Triglycerides (TGs) and cholesterol were measured using Thermo
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somatotroph transdifferentiation to these processes. To identify a potential transdifferentiating cell population, a 3.2 kb fragment of the rat PRL promoter that was able to faithfully reproduce endogenous PRL promoter activity ( Nelson et al . 1986 ) was
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2003 Regulation of serum leptin and its role in the hyperphagia of lactation in the rat . Journal of Endocrinology 176 193 – 203 . ( doi:10.1677/joe.0.1760193 ) De Roux N Genin E Carel J-C Matsuda F Chaussain J-L Milgrom E 2003
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offspring also did not differ from that of HV offspring. Figure 1 Short-term (1-h) food intake response, in grams, after i.p. injections of 0.9% saline and mCPP (2.5 mg/kg) at 6 weeks post-weaning in (A) male and (B) female offspring from Wistar rat
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, Beishuizen et al . 2003, John & Buckingham 2003 ). Evidence from the study on the rat has suggested that higher doses of LPS, as used in this study, may activate hypothalamic AVP synthesis and release ( Xia & Krukoff 2003 ), and that AVP is a major
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-H295R cells and a DHEA-induced rat model of PCOS. Our data may shed light on the pharmacological effects of baicalin and provide a scientific basis for its clinical applications. Materials and methods Cell culture and cytotoxicity assay The
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−/− mice ( Sowden et al . 2007 ). Further studies are required to investigate the effect of OXM on the heart, as no increase in heart rate was detected following administration of OXM in rat and humans ( Wynne et al . 2005 , 2006 , Sowden et al . 2007
The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, UK
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The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, UK
Zhejiang University-University of Edinburgh Institute, International Campus, Haining, Zhejiang, P.R. China
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fetal circulation. The second protective mechanism lies in the placenta, the interface that controls the maternal–fetal exchange of substances. The rat placenta can be divided into two zones: the junctional zone, which contains spongiotrophoblasts
Department of Neurosurgery, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan
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The blood samples were immediately centrifuged, and the plasma was stored at −20°C. Plasma LH concentrations were measured using a rat-specific double-antibody RIA kit (National Hormone and Peptide Program, Baltimore, MD, USA). Values were expressed in