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/mL insulin and 2.5 μM rosiglitazone was changed every 2 days. The cells were divided into two groups and were infected with adenovirus on day 6 and the culture medium was changed to 2% (w/v) fatty acid-free BSA on day 8. Cells were treated with or without 8
Department of Nutrition, Department of Nutrition and Food Hygiene, Department of Preventive Medicine, Department of Nutrition and Food Hygiene, Department of Medicine, Department of Medicine (Endocrinology), Department of Medicine (Endocrinology and Metabolism), Gillings School of Global Public Health, Nutrition Research Institute (NRI) at Kannapolis, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27559, USA
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Department of Nutrition, Department of Nutrition and Food Hygiene, Department of Preventive Medicine, Department of Nutrition and Food Hygiene, Department of Medicine, Department of Medicine (Endocrinology), Department of Medicine (Endocrinology and Metabolism), Gillings School of Global Public Health, Nutrition Research Institute (NRI) at Kannapolis, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27559, USA
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Department of Nutrition, Department of Nutrition and Food Hygiene, Department of Preventive Medicine, Department of Nutrition and Food Hygiene, Department of Medicine, Department of Medicine (Endocrinology), Department of Medicine (Endocrinology and Metabolism), Gillings School of Global Public Health, Nutrition Research Institute (NRI) at Kannapolis, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27559, USA
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Department of Nutrition, Department of Nutrition and Food Hygiene, Department of Preventive Medicine, Department of Nutrition and Food Hygiene, Department of Medicine, Department of Medicine (Endocrinology), Department of Medicine (Endocrinology and Metabolism), Gillings School of Global Public Health, Nutrition Research Institute (NRI) at Kannapolis, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27559, USA
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Department of Nutrition, Department of Nutrition and Food Hygiene, Department of Preventive Medicine, Department of Nutrition and Food Hygiene, Department of Medicine, Department of Medicine (Endocrinology), Department of Medicine (Endocrinology and Metabolism), Gillings School of Global Public Health, Nutrition Research Institute (NRI) at Kannapolis, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27559, USA
Department of Nutrition, Department of Nutrition and Food Hygiene, Department of Preventive Medicine, Department of Nutrition and Food Hygiene, Department of Medicine, Department of Medicine (Endocrinology), Department of Medicine (Endocrinology and Metabolism), Gillings School of Global Public Health, Nutrition Research Institute (NRI) at Kannapolis, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27559, USA
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primary mechanism of insulin resistance has not been established. The primary cause or the original root of insulin resistance and T2DM is ‘excess calories’, which derive from three main dietary components: glucose/carbohydrates, fat/fatty acids, and
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German Center for Diabetes Research (DZD), Neuherberg, Germany
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German Center for Diabetes Research (DZD), Neuherberg, Germany
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with equal potency in vitro ( Finan et al. 2013 ). Other modifications included an Aib residue at position 2 to increase resistance to DPP-IV cleavage. This peptide was either acylated with a C16:0 fatty acid (acylated version) at Lys40 or PEGylated
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roles in fetal growth during pregnancy ( Koletzko et al. 2007 ). Maternal triglyceride/lipids must be processed and transferred via several placental and endothelial lipases, fatty acid-binding proteins, and translocases ( Brett et al. 2014
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(triacyglycerols, free fatty acids and sterol esters), and total polar lipids were separated by thin-layer chromatography and quantified by their fatty acid contents. Fatty acid methyl esters were separated on a Clarus 500 gas chromatograph (PerkinElmer) identified
Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada
Department of Pharmacology, University of Toronto, Toronto, Ontario, Canada
Department of Psychiatry, University of Toronto, Toronto, Ontario, Canada
Banting & Best Diabetes Centre, Toronto, Ontario, Canada
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Banting & Best Diabetes Centre, Toronto, Ontario, Canada
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). In healthy humans and rodents during the hyperinsulinemic euglycemic clamp, inhibition of EGP is accompanied by a robust decrease in plasma concentrations of glycerol and free fatty acids, which is due to inhibition of adipose tissue lipolysis by
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protein (CREB) and nuclear localisation of cAMP-responsive CREB coactivator 2 (TORC2) in human adipocytes ( Kim et al. 2010 ). GIPR agonism promotes fatty acid uptake ( Killion et al. 2020 a ), insulin-induced free fatty acid incorporation into
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Preemptive Medicine and Lifestyle Related Disease Research Center, Kyoto University Hospital, Kyoto, Japan
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Iida K Suzuki K Hamasaki A Joo E Nasteska D Shibue K , et al . 2015 Free fatty acid receptor GPR120 is highly expressed in enteroendocrine K-cells of upper small intestine and has a critical role in GIP secretion after fat ingestion
The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel
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The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel
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duplicates in each membrane) was included in the densitometry calculations. Lipolysis assay Adipose tissue CM (100 mg tissue/mL) from the 3 groups was used for free fatty acids (FFA) measurement (NEFA-HR kit, Wako Diagnostics), according to the
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-resolution respirometry in the presence of ADP, n = 9–10 per group. FAO, fatty acid (octanoyl-carnitine) oxidation, CI+II oxidation of octanoyl-carnitine, malate, and glutamate, ETS electron transport system maximum respiratory capacity induced by uncoupling of