F508del beta cells have defective exocytosis and insulin secretion. Finally, we measured islet glucagon and somatostatin secretion. The data presented highlight the important contribution of CFTR in beta cells for functional insulin secretion
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A Edlund, M Barghouth, M Hühn, M Abels, J S E Esguerra, I G Mollet, E Svedin, A Wendt, E Renström, E Zhang, N Wierup, B J Scholte, M Flodström-Tullberg, and L Eliasson
Alessandro Pocai
insulin. Recent work on understanding the physiological function of proglucagon-derived peptides has renewed interest in glucagon-based therapeutics. One of these peptides is glucagon-like peptide-1 (GLP1), which is secreted from the L cells of the
S J Brandt, M Kleinert, M H Tschöp, and T D Müller
). For example, following Roux-en-Y gastric bypass, gastric banding or sleeve gastronomy, there is an increase in the secretion of glucagon-like peptide 1 (GLP-1) ( Laferrere 2016 , Meek et al. 2016 , Clemmensen et al. 2017 ), which is known not
Eun Young Lee, Shuji Kaneko, Promsuk Jutabha, Xilin Zhang, Susumu Seino, Takahito Jomori, Naohiko Anzai, and Takashi Miki
Introduction Oral ingestion of nutrients triggers the secretion of gut hormones from various enteroendocrine cells ( Ezcurra et al . 2013 , Cho et al . 2014 ). Among these, glucagon-like peptide 1 (GLP1) and glucose-dependent insulinotropic
Bernadette M Trojanowski, Heba H Salem, Heike Neubauer, Eric Simon, Martin Wagner, Rajkumar Dorajoo, Bernhard O Boehm, Leticia Labriola, Thomas Wirth, and Bernd Baumann
min. Insulin was determined in plasma samples as described previously ( Salem et al. 2014 ) using the Ultra-Sensitive Mouse Insulin ELISA Kit (Chrystal Chem, Elk Grove Village, IL, USA). Plasma glucagon concentration was evaluated using the Cisbio
Alyce M Martin, Emily W Sun, and Damien J Keating
basolateral stimuli. The characterisation of distinct EE cell types has been traditionally based on their dominant and supposedly unique hormone expression profile, such as enterochromaffin (EC) cells secreting serotonin (5-HT), L cells secreting glucagon
Esther Nuñez-Durán, Belén Chanclón, Silva Sütt, Joana Real, Hanns-Ulrich Marschall, Ingrid Wernstedt Asterholm, Emmelie Cansby, and Margit Mahlapuu
assessed in full pancreatic sections stained with hematoxylin-eosin (H-E; Histolab Products). For immunofluorescence, sections were incubated with primary antibodies for STK25, insulin, glucagon or α-smooth muscle actin (α-SMA), followed by incubation with
Anne-Marie O'Carroll, Gillian M Howell, Emma M Roberts, and Stephen J Lolait
(InsP 3 ) production, elevation of intracellular Ca 2 + , and activation of protein kinase C (PKC; Lang 1999 , Ahren et al . 2006 , Ramracheya et al . 2006 ). Other potentiators, such as the incretin hormones, glucagon-like peptide 1 and glucose
Yoshinori Kanemaru, Norio Harada, Satoko Shimazu-Kuwahara, Shunsuke Yamane, Eri Ikeguchi, Yuki Murata, Sakura Kiyobayashi, Tomonobu Hatoko, and Nobuya Inagaki
GIP levels, and 6 g glucose/body weight (kg) for plasma glucagon-like peptide-1 (GLP-1) levels) were performed. Blood samples were collected from the tail vein at 0, 15, 30, 60, and 120 min after oral glucose administration by oral gavage. Blood
Karen Piper Hanley, Tom Hearn, Andrew Berry, Melanie J Carvell, Ann-Marie Patch, Louise J Williams, Sarah A Sugden, David I Wilson, Sian Ellard, and Neil A Hanley
insulin and glucagon ( Fig 6 E–J). In the adult pancreas, RAB3B extensively co-localised with insulin but was somewhat variable in different β-cells ( Fig. 7 A–C). Some RAB3B was present in some α- and δ-cell but at relatively low level ( Fig. 7 D–I). In
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