Search Results
A Tsuchiya
A Tsuchiya
Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
Search for other papers by A Tsuchiya in
Google Scholar
PubMed
Search for other papers by A Tsuchiya in
Google Scholar
PubMed
T Kanno
T Kanno
Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
Search for other papers by T Kanno in
Google Scholar
PubMed
Search for other papers by T Kanno in
Google Scholar
PubMed
T Nishizaki
T Nishizaki
Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
Search for other papers by T Nishizaki in
Google Scholar
PubMed
Search for other papers by T Nishizaki in
Google Scholar
PubMed
Introduction Akt is a serine/threonine protein kinase bearing multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. Akt includes three closely related isoforms Akt1, Akt2, and Akt3
Manon M Roustit
Manon M Roustit
Search for other papers by Manon M Roustit in
Google Scholar
PubMed
Search for other papers by Manon M Roustit in
Google Scholar
PubMed
Joan M Vaughan
Joan M Vaughan
Department of Comparative Biomedical Sciences, Laboratory of Neuronal Structure and Function, Queen's Medical Research Institute, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by Joan M Vaughan in
Google Scholar
PubMed
Search for other papers by Joan M Vaughan in
Google Scholar
PubMed
Pauline M Jamieson
Pauline M Jamieson
Department of Comparative Biomedical Sciences, Laboratory of Neuronal Structure and Function, Queen's Medical Research Institute, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by Pauline M Jamieson in
Google Scholar
PubMed
Search for other papers by Pauline M Jamieson in
Google Scholar
PubMed
Mark E Cleasby
Mark E Cleasby
Search for other papers by Mark E Cleasby in
Google Scholar
PubMed
Search for other papers by Mark E Cleasby in
Google Scholar
PubMed
/2, AKT and PKCε activation ( Brar et al . 2002 , 2004 , Lawrence et al . 2005 ). UCN2 and CRFR2 are both expressed in mouse skeletal muscle ( Chen et al . 2004 , Keipert et al . 2013 ), where they inhibit atrophy and promote hypertrophy ( Hinkle
Corinne Caillaud
Corinne Caillaud
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Corinne Caillaud in
Google Scholar
PubMed
Search for other papers by Corinne Caillaud in
Google Scholar
PubMed
Mie Mechta
Mie Mechta
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Mie Mechta in
Google Scholar
PubMed
Search for other papers by Mie Mechta in
Google Scholar
PubMed
Heidi Ainge
Heidi Ainge
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Heidi Ainge in
Google Scholar
PubMed
Search for other papers by Heidi Ainge in
Google Scholar
PubMed
Andreas N Madsen
Andreas N Madsen
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Andreas N Madsen in
Google Scholar
PubMed
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Andreas N Madsen in
Google Scholar
PubMed
Patricia Ruell
Patricia Ruell
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Patricia Ruell in
Google Scholar
PubMed
Search for other papers by Patricia Ruell in
Google Scholar
PubMed
Emilie Mas
Emilie Mas
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Emilie Mas in
Google Scholar
PubMed
Search for other papers by Emilie Mas in
Google Scholar
PubMed
Catherine Bisbal
Catherine Bisbal
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Catherine Bisbal in
Google Scholar
PubMed
Search for other papers by Catherine Bisbal in
Google Scholar
PubMed
Jacques Mercier
Jacques Mercier
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Jacques Mercier in
Google Scholar
PubMed
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Jacques Mercier in
Google Scholar
PubMed
Stephen Twigg
Stephen Twigg
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Stephen Twigg in
Google Scholar
PubMed
Search for other papers by Stephen Twigg in
Google Scholar
PubMed
Trevor A Mori
Trevor A Mori
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Trevor A Mori in
Google Scholar
PubMed
Search for other papers by Trevor A Mori in
Google Scholar
PubMed
David Simar
David Simar
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by David Simar in
Google Scholar
PubMed
Search for other papers by David Simar in
Google Scholar
PubMed
Romain Barrès
Romain Barrès
Exercise Health and Performance, Faculty of Health and Medical Sciences, Department of Neuroscience and Pharmacology, School of Medicine and Pharmacology, UMR CNRS 9214, Physiology Department, Department of Endocrinology, Inflammation and Infection Research, Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Romain Barrès in
Google Scholar
PubMed
Search for other papers by Romain Barrès in
Google Scholar
PubMed
), SPA-810, 1/2000, Victoria, BC, Canada) or Cell Signaling (AKT (9272), P-AKT (Ser473) (9271), Danvers, MA, USA), and secondary antibodies were purchased from Sigma–Aldrich (sheep anti-mouse, A5906) or Cell Signaling (goat anti-rabbit, 7074). Serum
M E Cleasby
M E Cleasby
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by M E Cleasby in
Google Scholar
PubMed
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by M E Cleasby in
Google Scholar
PubMed
Q Lau
Q Lau
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by Q Lau in
Google Scholar
PubMed
Search for other papers by Q Lau in
Google Scholar
PubMed
E Polkinghorne
E Polkinghorne
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by E Polkinghorne in
Google Scholar
PubMed
Search for other papers by E Polkinghorne in
Google Scholar
PubMed
S A Patel
S A Patel
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by S A Patel in
Google Scholar
PubMed
Search for other papers by S A Patel in
Google Scholar
PubMed
S J Leslie
S J Leslie
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by S J Leslie in
Google Scholar
PubMed
Search for other papers by S J Leslie in
Google Scholar
PubMed
N Turner
N Turner
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by N Turner in
Google Scholar
PubMed
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by N Turner in
Google Scholar
PubMed
G J Cooney
G J Cooney
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by G J Cooney in
Google Scholar
PubMed
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by G J Cooney in
Google Scholar
PubMed
A Xu
A Xu
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by A Xu in
Google Scholar
PubMed
Search for other papers by A Xu in
Google Scholar
PubMed
E W Kraegen
E W Kraegen
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by E W Kraegen in
Google Scholar
PubMed
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Search for other papers by E W Kraegen in
Google Scholar
PubMed
act as a dynamic scaffold for endosomal signalling proteins ( Miaczynska et al . 2004 , Chial et al . 2008 ) and was first identified as an interacting partner for the serine/threonine kinase AKT2 ( Mitsuuchi et al . 1999 ). AKT2 is required for
Lei Zhang
Lei Zhang
School of Medicine, Centre for Endocrine and Diabetes Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Search for other papers by Lei Zhang in
Google Scholar
PubMed
Search for other papers by Lei Zhang in
Google Scholar
PubMed
Carol Paddon
Carol Paddon
School of Medicine, Centre for Endocrine and Diabetes Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Search for other papers by Carol Paddon in
Google Scholar
PubMed
Search for other papers by Carol Paddon in
Google Scholar
PubMed
Mark D Lewis
Mark D Lewis
School of Medicine, Centre for Endocrine and Diabetes Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Search for other papers by Mark D Lewis in
Google Scholar
PubMed
Search for other papers by Mark D Lewis in
Google Scholar
PubMed
Fiona Grennan-Jones
Fiona Grennan-Jones
School of Medicine, Centre for Endocrine and Diabetes Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Search for other papers by Fiona Grennan-Jones in
Google Scholar
PubMed
Search for other papers by Fiona Grennan-Jones in
Google Scholar
PubMed
Marian Ludgate
Marian Ludgate
School of Medicine, Centre for Endocrine and Diabetes Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Search for other papers by Marian Ludgate in
Google Scholar
PubMed
Search for other papers by Marian Ludgate in
Google Scholar
PubMed
Signalling Technology unless otherwise stated): anti phospho-CREB (Ser 133, 1:2000 overnight; 4 °C); anti total-CREB (1:1000, room temperature, 1 h; Santa CruzBiotechnology, Santa Cruz, CA, USA); anti phospho-Akt (Thr 308, 1:1000, 4 °C, overnight); anti total-Akt
Thomas Nicholson
Thomas Nicholson
Institute of Inflammation and Ageing, MRC-ARUK Centre for Musculoskeletal Ageing Research, University of Birmingham, Birmingham, UK
Search for other papers by Thomas Nicholson in
Google Scholar
PubMed
Search for other papers by Thomas Nicholson in
Google Scholar
PubMed
Chris Church
Chris Church
MedImmune, Cardiovascular and Metabolic Disease (CVMD), Milstein Building, Cambridge, UK
Search for other papers by Chris Church in
Google Scholar
PubMed
Search for other papers by Chris Church in
Google Scholar
PubMed
Kostas Tsintzas
Kostas Tsintzas
MRC-ARUK Centre for Musculoskeletal Ageing Research, School of Life Sciences, Faculty of Medicine & Health Sciences, University of Nottingham, Nottingham, UK
Search for other papers by Kostas Tsintzas in
Google Scholar
PubMed
Search for other papers by Kostas Tsintzas in
Google Scholar
PubMed
Robert Jones
Robert Jones
MRC-ARUK Centre for Musculoskeletal Ageing Research, School of Life Sciences, Faculty of Medicine & Health Sciences, University of Nottingham, Nottingham, UK
Search for other papers by Robert Jones in
Google Scholar
PubMed
Search for other papers by Robert Jones in
Google Scholar
PubMed
Leigh Breen
Leigh Breen
Institute of Inflammation and Ageing, MRC-ARUK Centre for Musculoskeletal Ageing Research, University of Birmingham, Birmingham, UK
Search for other papers by Leigh Breen in
Google Scholar
PubMed
Search for other papers by Leigh Breen in
Google Scholar
PubMed
Edward T Davis
Edward T Davis
The Royal Orthopaedic Hospital NHS Foundation Trust, Northfield, Birmingham, UK
Search for other papers by Edward T Davis in
Google Scholar
PubMed
Search for other papers by Edward T Davis in
Google Scholar
PubMed
David J Baker
David J Baker
MedImmune, Cardiovascular and Metabolic Disease (CVMD), Milstein Building, Cambridge, UK
Search for other papers by David J Baker in
Google Scholar
PubMed
Search for other papers by David J Baker in
Google Scholar
PubMed
Simon W Jones
Simon W Jones
Institute of Inflammation and Ageing, MRC-ARUK Centre for Musculoskeletal Ageing Research, University of Birmingham, Birmingham, UK
Search for other papers by Simon W Jones in
Google Scholar
PubMed
Search for other papers by Simon W Jones in
Google Scholar
PubMed
(1:100 dilution, Sigma-Aldrich). Insulin receptor, IGF1 receptor and AKT (thr308) phosphorylation, as a percentage of total protein, was determined using MesoScale Discovery phospho and total protein assays, performed according to the manufacturer
Mark E Cleasby
Mark E Cleasby
Department of Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, UK
Search for other papers by Mark E Cleasby in
Google Scholar
PubMed
Search for other papers by Mark E Cleasby in
Google Scholar
PubMed
Pauline M Jamieson
Pauline M Jamieson
Centre for Cardiovascular Science, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, UK
Search for other papers by Pauline M Jamieson in
Google Scholar
PubMed
Search for other papers by Pauline M Jamieson in
Google Scholar
PubMed
Philip J Atherton
Philip J Atherton
Division of Medical Sciences and Graduate Entry Medicine, University of Nottingham, Medical School, Royal Derby Hospital, Derby, UK
Search for other papers by Philip J Atherton in
Google Scholar
PubMed
Search for other papers by Philip J Atherton in
Google Scholar
PubMed
expression or activity. Broken arrow: indirect effect. P indicates phosphorylation. ACT2BR, activin 2B receptor; AMPK, AMP-activated protein kinase; AS160, Akt substrate of 160 kDa; CRFR2, corticotrophin-releasing factor receptor 2; FOXO, forkhead
Amanda E Garza
Amanda E Garza
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Amanda E Garza in
Google Scholar
PubMed
Search for other papers by Amanda E Garza in
Google Scholar
PubMed
Elijah Trefts
Elijah Trefts
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Elijah Trefts in
Google Scholar
PubMed
Search for other papers by Elijah Trefts in
Google Scholar
PubMed
Isis A Katayama Rangel
Isis A Katayama Rangel
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Isis A Katayama Rangel in
Google Scholar
PubMed
Search for other papers by Isis A Katayama Rangel in
Google Scholar
PubMed
Danielle Brooks
Danielle Brooks
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Danielle Brooks in
Google Scholar
PubMed
Search for other papers by Danielle Brooks in
Google Scholar
PubMed
Rene Baudrand
Rene Baudrand
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Department of Endocrinology, School of Medicine, Pontificia Universidad Catolica De Chile, Santiago, Chile
Search for other papers by Rene Baudrand in
Google Scholar
PubMed
Department of Endocrinology, School of Medicine, Pontificia Universidad Catolica De Chile, Santiago, Chile
Search for other papers by Rene Baudrand in
Google Scholar
PubMed
Burhanuddin Moize
Burhanuddin Moize
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Burhanuddin Moize in
Google Scholar
PubMed
Search for other papers by Burhanuddin Moize in
Google Scholar
PubMed
Jose R Romero
Jose R Romero
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Jose R Romero in
Google Scholar
PubMed
Search for other papers by Jose R Romero in
Google Scholar
PubMed
Sanjay Ranjit
Sanjay Ranjit
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Sanjay Ranjit in
Google Scholar
PubMed
Search for other papers by Sanjay Ranjit in
Google Scholar
PubMed
Thitinan Treesaranuwattana
Thitinan Treesaranuwattana
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Thitinan Treesaranuwattana in
Google Scholar
PubMed
Search for other papers by Thitinan Treesaranuwattana in
Google Scholar
PubMed
Tham M Yao
Tham M Yao
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Tham M Yao in
Google Scholar
PubMed
Search for other papers by Tham M Yao in
Google Scholar
PubMed
Gail K Adler
Gail K Adler
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Gail K Adler in
Google Scholar
PubMed
Search for other papers by Gail K Adler in
Google Scholar
PubMed
Luminita H Pojoga
Luminita H Pojoga
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Luminita H Pojoga in
Google Scholar
PubMed
Search for other papers by Luminita H Pojoga in
Google Scholar
PubMed
Gordon H Williams
Gordon H Williams
Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for other papers by Gordon H Williams in
Google Scholar
PubMed
Search for other papers by Gordon H Williams in
Google Scholar
PubMed
, magnification 600×. Data presented as mean ± s.e.m. , n = 6–8 mice/group. (D) pAkt/Akt renal cortex protein analysis by western immunoblot. Data presented as mean ± s.e.m. , n = 6–8 mice/group. Statistical analysis, * = P < 0.05, ** = P < 0
Nami Kim
Nami Kim
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Nami Kim in
Google Scholar
PubMed
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Nami Kim in
Google Scholar
PubMed
Jung Ok Lee
Jung Ok Lee
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Jung Ok Lee in
Google Scholar
PubMed
Search for other papers by Jung Ok Lee in
Google Scholar
PubMed
Hye Jeong Lee
Hye Jeong Lee
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Hye Jeong Lee in
Google Scholar
PubMed
Search for other papers by Hye Jeong Lee in
Google Scholar
PubMed
Yong Woo Lee
Yong Woo Lee
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Yong Woo Lee in
Google Scholar
PubMed
Search for other papers by Yong Woo Lee in
Google Scholar
PubMed
Hyung Ip Kim
Hyung Ip Kim
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Hyung Ip Kim in
Google Scholar
PubMed
Search for other papers by Hyung Ip Kim in
Google Scholar
PubMed
Su Jin Kim
Su Jin Kim
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Su Jin Kim in
Google Scholar
PubMed
Search for other papers by Su Jin Kim in
Google Scholar
PubMed
Sun Hwa Park
Sun Hwa Park
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Sun Hwa Park in
Google Scholar
PubMed
Search for other papers by Sun Hwa Park in
Google Scholar
PubMed
Chul Su Lee
Chul Su Lee
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Chul Su Lee in
Google Scholar
PubMed
Search for other papers by Chul Su Lee in
Google Scholar
PubMed
Sun Woo Ryoo
Sun Woo Ryoo
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Sun Woo Ryoo in
Google Scholar
PubMed
Search for other papers by Sun Woo Ryoo in
Google Scholar
PubMed
Geum-Sook Hwang
Geum-Sook Hwang
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Geum-Sook Hwang in
Google Scholar
PubMed
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Geum-Sook Hwang in
Google Scholar
PubMed
Hyeon Soo Kim
Hyeon Soo Kim
Department of Anatomy, Department of Medicine, Integrated Metabolomics Research Group, Department of Life Science, Korea University College of Medicine, Seoul 136-701, South Korea
Search for other papers by Hyeon Soo Kim in
Google Scholar
PubMed
Search for other papers by Hyeon Soo Kim in
Google Scholar
PubMed
Isoeugenol exerts various beneficial effects on human health. However, the mechanisms underlying these effects are poorly understood. In this study, we observed that isoeugenol activated AMP-activated protein kinase (AMPK) and increased glucose uptake in rat L6 myotubes. Isoeugenol-induced increase in intracellular calcium concentration and glucose uptake was inhibited by STO-609, an inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). Isoeugenol also increased the phosphorylation of protein kinase C-α (PKCα). Chelation of calcium with BAPTA-AM blocked isoeugenol-induced AMPK phosphorylation and glucose uptake. Isoeugenol stimulated p38MAPK phosphorylation that was inhibited after pretreatment with compound C, an AMPK inhibitor. Isoeugenol also increased glucose transporter type 4 (GLUT4) expression and its translocation to the plasma membrane. GLUT4 translocation was not observed after the inhibition of AMPK and CaMKK. In addition, isoeugenol activated the Akt substrate 160 (AS160) pathway, which is downstream of the p38MAPK pathway. Knockdown of the gene encoding AS160 inhibited isoeugenol-induced glucose uptake. Together, these results indicate that isoeugenol exerts beneficial health effects by activating the AMPK/p38MAPK/AS160 pathways in skeletal muscle.
Sandra Pereira
Sandra Pereira
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Sandra Pereira in
Google Scholar
PubMed
Search for other papers by Sandra Pereira in
Google Scholar
PubMed
Wen Qin Yu
Wen Qin Yu
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Wen Qin Yu in
Google Scholar
PubMed
Search for other papers by Wen Qin Yu in
Google Scholar
PubMed
María E Frigolet
María E Frigolet
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by María E Frigolet in
Google Scholar
PubMed
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by María E Frigolet in
Google Scholar
PubMed
Jacqueline L Beaudry
Jacqueline L Beaudry
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Jacqueline L Beaudry in
Google Scholar
PubMed
Search for other papers by Jacqueline L Beaudry in
Google Scholar
PubMed
Yaniv Shpilberg
Yaniv Shpilberg
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Yaniv Shpilberg in
Google Scholar
PubMed
Search for other papers by Yaniv Shpilberg in
Google Scholar
PubMed
Edward Park
Edward Park
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Edward Park in
Google Scholar
PubMed
Search for other papers by Edward Park in
Google Scholar
PubMed
Cristina Dirlea
Cristina Dirlea
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Cristina Dirlea in
Google Scholar
PubMed
Search for other papers by Cristina Dirlea in
Google Scholar
PubMed
B L Grégoire Nyomba
B L Grégoire Nyomba
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by B L Grégoire Nyomba in
Google Scholar
PubMed
Search for other papers by B L Grégoire Nyomba in
Google Scholar
PubMed
Michael C Riddell
Michael C Riddell
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Michael C Riddell in
Google Scholar
PubMed
Search for other papers by Michael C Riddell in
Google Scholar
PubMed
I George Fantus
I George Fantus
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by I George Fantus in
Google Scholar
PubMed
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by I George Fantus in
Google Scholar
PubMed
Adria Giacca
Adria Giacca
Department of Physiology, University of Toronto, Department of Medicine, Mount Sinai Hospital, Toronto General Research Institute and Banting and Best Diabetes Centre, University Health Network, Muscle Health Research Center and Physical Activity and Chronic Disease Unit, Faculty of Health, School of Kinesiology and Health Science, York University, Department of Internal Medicine, University of Manitoba, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8
Search for other papers by Adria Giacca in
Google Scholar
PubMed
Search for other papers by Adria Giacca in
Google Scholar
PubMed
serine 307-phosphorylated IRS-1 (Millipore, Temecula, CA, USA), rabbit polyclonal anti-IRS-1 antibody (Millipore), rabbit polyclonal antibody against serine 473-phosphorylated Akt (Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-Akt
