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In this study, we investigated the role of intercellular adhesion molecule-2 (ICAM2) in the testis. ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Herein, we showed ICAM2 to be expressed by germ and Sertoli cells in the rat testis. When a monospecific antibody was used for immunolocalization experiments, ICAM2 was found to surround the heads of elongating/elongated spermatids in all stages of the seminiferous epithelial cycle. To determine whether ICAM2 is a constituent of apical ectoplasmic specialization (ES), co-immunoprecipitation and dual immunofluorescence staining were performed. Interestingly, ICAM2 was found to associate with β1-integrin, nectin-3, afadin, Src, proline-rich tyrosine kinase 2, annexin II, and actin. Following CdCl2 treatment, ICAM2 was found to be upregulated during restructuring of the seminiferous epithelium, with round spermatids becoming increasingly immunoreactive for ICAM2 by 6–16 h. Interestingly, there was a loss in the binding of ICAM2 to actin during CdCl2-induced germ cell loss, suggesting that a loss of ICAM2–actin interactions might have facilitated junction restructuring. Taken collectively, these results illustrate that ICAM2 plays an important role in apical ES dynamics during spermatogenesis.
Department of Animal Sciences, Department of Molecular and Cellular Physiology, University of Arizona, Tucson, Arizona 85721, USA
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myoepithelial cells, and asteriks (*) correspond to vascular endothelium. Expression of HTR1B , 2A , 2B , 4 , and 7 was each localized in BMT ( Fig. 2 , row A, columns 1–5). Counterstaining with GS-I (vascular endothelium) indicated that HTR1B , 2A , and
St Mary’s Hospital, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK
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cytotrophoblast (EVT) cells invade the spiral arteries so that both the endothelium and smooth muscle of the vessels are eroded ( Burton et al . 2017 ). This invasion by the EVT is extensive and initially plugs the terminal end of the vessels, preventing any
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Mediation by M 3 -muscarinic receptors of both endothelium-dependent contraction and relaxation to acetylcholine in the aorta of the spontaneously hypertensive rat . British Journal of Pharmacology 112 519 – 524 . ( https://doi.org/10.1111/j.1476
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. 2018 ). In 1994 Desoye et al . reported that insulin receptor protein expression shifts from the maternal facing side of the placenta (the syncytiotrophoblast) to the fetal facing side of the placenta (the fetal endothelium) with advancing gestation in
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questions remain unanswered including the aforementioned. How is leptin transported into the CNS? Previous studies suggested that leptin entered the brain by transport across the vascular endothelium ( Banks & Farrell 1999 , Banks & Farrell 2003 ). However
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LS Nash GB McGettrick HM 2016 Comparative ability of mesenchymal stromal cells from different tissues to limit neutrophil recruitment to inflamed endothelium . PLoS ONE 11 e0155161 . ( doi:10.1371/journal.pone.0155161 ) Nagase H
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: MacGraw Hill . Fulton CT Stallone JN 2002 Sexual dimorphism in prostanoid-potentiated vascular contraction: roles of endothelium and ovarian steroids . American Journal of Physiology 283 H2062 – H2073 . Garcia
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technique uses fluorescent imaging probes that accumulate in BAT ( Azhdarinia et al. 2013 , Zhang et al. 2015 ). For example, PEP3 is a peptide that binds to receptors on the endothelium of BAT and beige adipose tissue, which when conjugated with a
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Beijing Institute of Hepatology, Beijing, China
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Beijing Lab for Cardiovascular Precision Medicine, Beijing, China
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cell-specific proteins, was detected. The results showed that the number of E-cadherin+ cells in the endothelium of arterioles of heart were significantly decreased in kkAy mice compared to C57 mice (diabetic effects, P <0.01), which were
