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Shuang-Xia Zhao, Shanli Tsui, Anthony Cheung, Raymond S Douglas, Terry J Smith and J Paul Banga

-713; Santa Cruz, Santa Cruz, CA, USA) served as positive and negative controls respectively. Histology Thyroid–trachea preparations were fixed in 10% buffered formalin, embedded in paraffin, and sectioned for hematoxylin and eosin (H&E) staining. To

Open access

Valentina Pampanini, Daniela Germani, Antonella Puglianiello, Jan-Bernd Stukenborg, Ahmed Reda, Iuliia Savchuk, Kristín Rós Kjartansdóttir, Stefano Cianfarani and Olle Söder

animals from at least three different litters. For the 5 d pp old animals, comparisons were made between seven IUGR and four shams, respecting the same principle of litter variability. Testicular histology and immunohistochemistry At killing

Open access

Xuefeng Yang, Shuang Mei, Haihua Gu, Huailan Guo, Longying Zha, Junwei Cai, Xuefeng Li, Zhenqi Liu and Wenhong Cao

-SOD. Histological examination of pancreas Pancreatic slices (5 mm thickness) were fixed in 10% neutral-buffered formalin/Bouin's fluid overnight. All the tissue samples were subjected to standard processing and staining procedures. Paraffin-embedded sections of 5

Open access

Esther Nuñez-Durán, Belén Chanclón, Silva Sütt, Joana Real, Hanns-Ulrich Marschall, Ingrid Wernstedt Asterholm, Emmelie Cansby and Margit Mahlapuu

collected for histological analysis (see Histology and immunofluorescence) or snap frozen in liquid nitrogen and stored at –80°C for the analysis of gene expression (see Supplementary Fig. 1 for schematic overview, see section on supplementary data given

Open access

Maayan Vatarescu, Sapir Bechor, Yulia Haim, Tal Pecht, Tanya Tarnovscki, Noa Slutsky, Ori Nov, Hagit Shapiro, Avishai Shemesh, Angel Porgador, Nava Bashan and Assaf Rudich

other loading tests (glucose, alanine, pyruvate or fructose, all at 2 g/kg). Blood was drawn from the tail at the indicated time points. Area under the glucose excursion curve (AUC) was calculated by GraphPad software (Prism 5). Histology

Open access

Ashley Patton, Tyler Church, Caroline Wilson, Jean Thuma, Douglas J Goetz, Darlene E Berryman, Edward O List, Frank Schwartz and Kelly D McCall

weight). Subsequent blood glucose measurements were performed at time 0 and at 20/30, 60, 90, 120 and 180 min post IP injection of the glucose bolus. Histological analysis For microscopic examination of liver morphology and steatosis, liver tissue

Open access

L A Nolan and A Levy

was designed to further define the short and longer term mitotic and apoptotic effects of continuous oestrogen exposure on the rat anterior pituitary. We have used both highly accurate direct histological assessment of mitotic index and BrdU

Open access

Noelia Martínez-Sánchez, José M Moreno-Navarrete, Cristina Contreras, Eva Rial-Pensado, Johan Fernø, Rubén Nogueiras, Carlos Diéguez, José-Manuel Fernández-Real and Miguel López

-like effector a (CIDEA, Hs00154455_m1). Gene expression values were expressed relative to PPIA levels. Histology and immunohistochemistry Adipose tissue depots were fixed in 10% buffered formaldehyde. For the hematoxylin–eosin processing, the WAT

Open access

T Mracek, D Gao, T Tzanavari, Y Bao, X Xiao, C Stocker, P Trayhurn and C Bing

collected at the time points of 0, 2, 4, 6, 8, 12, 24 and 48 h. Histology Liver samples from ob/ob and lean mice were fixed in 10% neutral formalin for 24 h, dehydrated in absolute ethanol, cleared in xylene and then embedded in paraffin. The paraffin was

Open access

Ya-Li Yang, Li-Rong Ren, Li-Feng Sun, Chen Huang, Tian-Xia Xiao, Bao-Bei Wang, Jie Chen, Brian A Zabel, Peigen Ren and Jian V Zhang

’s solution and embedded in paraffin for histological ­examination. Postpartum corpus luteum model Six- to 8-week-old females were housed with males, the occurrence of copulatory plugs was verified by visual examination at each day morning. Females on