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Hiroharu Mifune Institute of Animal Experimentation, Kurume University School of Medicine, Kurume, Japan

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Yuji Tajiri Division of Endocrinology and Metabolism, Kurume University School of Medicine, Kurume, Japan

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Yusuke Sakai Institute of Animal Experimentation, Kurume University School of Medicine, Kurume, Japan

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Yukie Kawahara Department of Pharmacology, Kurume University School of Medicine, Kurume, Japan

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Kento Hara Division of Endocrinology and Metabolism, Kurume University School of Medicine, Kurume, Japan

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Takahiro Sato Molecular Genetics, Life Science Institute, Kurume University, Kurume, Japan

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Yoshihiro Nishi Department of Physiology, Kurume University School of Medicine, Kurume, Japan

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Akinori Nishi Department of Pharmacology, Kurume University School of Medicine, Kurume, Japan

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Ryouichi Mitsuzono Department of Exercise Physiology, Institute of Health and Sports Science, Kurume University, Kurume, Japan

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Tatsuyuki Kakuma Bostatistics Center, Kurume University, Kurume, Japan

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Masayasu Kojima Molecular Genetics, Life Science Institute, Kurume University, Kurume, Japan

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-dark cycle (light on 07:00–19:00 h) with ad libitum or time-restricted feeding access to standard powder chow (10 kcal% fat, produced by Research Diets, Inc.: open source diet code D12450B) and water. All the experiments were performed in accordance with

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Taira Wada Laboratory of Health Science, School of Pharmacy, Nihon University, Funabshi, Chiba, Japan

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Yukiko Yamamoto Laboratory of Health Science, School of Pharmacy, Nihon University, Funabshi, Chiba, Japan

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Yukiko Takasugi Laboratory of Health Science, School of Pharmacy, Nihon University, Funabshi, Chiba, Japan

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Hirotake Ishii Laboratory of Health Science, School of Pharmacy, Nihon University, Funabshi, Chiba, Japan

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Taketo Uchiyama Laboratory of Organic Chemistry, School of Pharmacy, Nihon University, Funabshi, Chiba, Japan

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Kaori Saitoh Department of Psychiatry, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan

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Masahiro Suzuki Department of Psychiatry, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan

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Makoto Uchiyama Department of Psychiatry, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan
Tokyo Adachi Hospital, Adachi, Tokyo, Japan

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Hikari Yoshitane Department of Biological Sciences, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

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Yoshitaka Fukada Department of Biological Sciences, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

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Shigeki Shimba Laboratory of Health Science, School of Pharmacy, Nihon University, Funabshi, Chiba, Japan

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(+/+) mice and the APN (−/−) mice are similar to this behavioral pattern, that is, the amount of food intake by the APN (+/+) mice decreased between CT14 and CT16, showing a bimodal pattern, whereas the APN (−/−) mice were still feeding at that time period

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Elizabeth M Simpson School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW, Australia
Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW, Australia

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Iain J Clarke School of Agriculture Food and Ecosystem Sciences, Faculty of Science, The University of Melbourne, Parkville, VIC, Australia

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Christopher J Scott School of Dentistry and Medical Science, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW, Australia

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Cyril P Stephen School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW, Australia
Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW, Australia

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Alexandra Rao School of Agriculture Food and Ecosystem Sciences, Faculty of Science, The University of Melbourne, Parkville, VIC, Australia

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Allan J Gunn School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW, Australia
Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW, Australia

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with an LH surge were excluded from analysis. Analysis was restricted to the period during which sampling occurred every 10 min. Inter-pulse interval (IPI) was calculated as the interval in time between the start of two adjacent pulses. A mixed

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Keld Fosgerau Novo Nordisk Diabetes Research Unit, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark

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Kirsten Raun Novo Nordisk Diabetes Research Unit, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark

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Cecilia Nilsson Novo Nordisk Diabetes Research Unit, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark

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Kirsten Dahl Novo Nordisk Diabetes Research Unit, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark

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Birgitte S Wulff Novo Nordisk Diabetes Research Unit, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark

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responses such as pigmentation ( Lerner & McGuire 1961 ), sexual behavior ( Bertolini et al . 1968 ), thermoregulation, and inflammatory responses, but also feeding ( Vergoni et al . 1986 ) and control of body weight ( Fan et al . 1997 , MacNeil et al

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F Wahab Stem Cell Biology Unit, Laboratory of Reproductive Neuroendocrinology, Leibniz Institute for Primate Research, German Primate Center, Kellnerweg 4, D-37077 Göttingen, Germany

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M Shahab Stem Cell Biology Unit, Laboratory of Reproductive Neuroendocrinology, Leibniz Institute for Primate Research, German Primate Center, Kellnerweg 4, D-37077 Göttingen, Germany

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R Behr Stem Cell Biology Unit, Laboratory of Reproductive Neuroendocrinology, Leibniz Institute for Primate Research, German Primate Center, Kellnerweg 4, D-37077 Göttingen, Germany

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KP containing cells in the brain center for feeding and reproduction control Besides many other functions, the hypothalamus is the brain center for the regulation of feeding and reproduction ( Kennedy & Mitra 1963 b , Wynne et al . 2005 ). It serves

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Gencer Sancar German Center for Diabetes Research, Neuherberg, Germany
Department of Internal Medicine IV, Division of Diabetology, Endocrinology and Nephrology, Eberhard-Karls University of Tübingen, Tübingen, Germany
Institute for Diabetes Research and Metabolic Diseases, Helmholtz Center Munich, Eberhard-Karls University of Tübingen, Tübingen, Germany

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Andreas L Birkenfeld German Center for Diabetes Research, Neuherberg, Germany
Department of Internal Medicine IV, Division of Diabetology, Endocrinology and Nephrology, Eberhard-Karls University of Tübingen, Tübingen, Germany
Institute for Diabetes Research and Metabolic Diseases, Helmholtz Center Munich, Eberhard-Karls University of Tübingen, Tübingen, Germany

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Beyl R Early KS Cefalu WT Ravussin E & Peterson CM 2018 Early time-restricted feeding improves insulin sensitivity, blood pressure, and oxidative stress even without weight loss in men with prediabetes . Cell Metabolism 27 1212 – 1221.e3

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S Schmidt Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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A Hommel Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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V Gawlik Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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R Augustin Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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N Junicke Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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S Florian Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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M Richter Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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D J Walther Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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D Montag Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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H-G Joost Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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A Schürmann Toxicology, Department of Human Molecular Genetics, Research Group Neurogenetics, Departments of

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development. However, despite cell death, Slc2a3 −/− embryos implanted but were lost at embryonic day 8.5 ( Ganguly et al . 2007 ). Here, we describe the exact time point of embryonic lethality, and identify the defect responsible for death of post

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Romain Fontaine Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway

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Eirill Ager-Wick Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway

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Kjetil Hodne Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway

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Finn-Arne Weltzien Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway

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) were maintained at 28°C on a 14/10 h light/dark cycle in a re-circulating system with reverse osmosis dosed-salt water (pH 7.6 and conductivity of 800 µs). Fish were fed three times a day, once with live brine shrimp and twice with dry feed (Gemma

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M A Hyatt Early Life Nutrition Research Unit, Respiratory Biomedical Research Unit, Department of Animal Sciences, Division of Human Development, Academic Child Health

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D H Keisler Early Life Nutrition Research Unit, Respiratory Biomedical Research Unit, Department of Animal Sciences, Division of Human Development, Academic Child Health

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H Budge Early Life Nutrition Research Unit, Respiratory Biomedical Research Unit, Department of Animal Sciences, Division of Human Development, Academic Child Health
Early Life Nutrition Research Unit, Respiratory Biomedical Research Unit, Department of Animal Sciences, Division of Human Development, Academic Child Health

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M E Symonds Early Life Nutrition Research Unit, Respiratory Biomedical Research Unit, Department of Animal Sciences, Division of Human Development, Academic Child Health
Early Life Nutrition Research Unit, Respiratory Biomedical Research Unit, Department of Animal Sciences, Division of Human Development, Academic Child Health

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calculated using the 2 −Δ C t method ( Livak & Schmittgen 2001 ). Gene expression data are normalised to the 1-day-old group born to multiparous mothers and are presented as a fold change. Quantitative real-time PCR analysis The relative abundance of GR, HSD

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Pauline Campos College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, UK

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Jamie J Walker College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, UK
EPSRC Centre for Predictive Modelling in Healthcare, University of Exeter, Exeter, UK
Bristol Medical School, Translational Health Sciences, University of Bristol, Bristol, UK

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Patrice Mollard IGF, University of Montpellier, CNRS, INSERM, Montpellier, France

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the impact of neuronal activity within a network on other functions. This methodology has been successfully used to study arcuate nucleus and amygdala control of feeding behaviour in freely moving mice ( Betley et al. 2015 , Jennings et al. 2015

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