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deletion analysis and transcription factor-binding site analysis, GATA-binding factor 1 (GATA1) was identified as a transcription factor most relevant to the HSD3B2 gene expression after cells were incubated with baicalin. To further confirm these results
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-Akt (1:1000, room temperature, 1 h, Santa Cruz); and anti phospho-SYK (Tyr 352, 1:5000, overnight, 4 °C, BD Biosciences, San Jose, CA, USA). To investigate transcription factors, rabbit antibodies to phospho-PPARγ (Ser 82, 1:1000 overnight, 4 °C, Upstate
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) ( Garcia et al . 2014 ). Alternatively, as normal pituitary development depends on the sequential temporal and spatial expression of a cascade of signaling molecules and transcription factors, mutations in early ( HESX1, LHX3, LHX4, SOX3, OTX2 ) or late
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Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy
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oestrogen – are predominantly mediated through two distinct ERs (ERα and ERβ) that share a common phylogenetic origin, conserved structural organization and similar mode of action (mainly as ligand-operated transcription factors) with the other members of
Medicine, Faculty of Life Sciences, Centre for Molecular
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translocating to the nucleus, and interacting with other transcription factors and transcriptional modulators ( Chen et al . 2001 , Stevens et al . 2003 , Garside et al . 2004 , O'Malley 2005 , McMaster & Ray 2007 ). In vivo target cells are subject to
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-releasing hormone (CRH), brain-derived neurotropic factor (BDNF), and cytokines ( Goujon et al . 1997 , Schulkin et al . 1998 , Morsink et al . 2006 , Schulte-Herbruggen et al . 2006 ). The GR transcriptional activity is dependent on the cell type, the
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transcription factors in macrophages ( Tsatsanis et al . 2006 ). These data contrast with the reduced IRS1 and AKT phosphorylation observed in the transgenic mice ( Jamieson et al . 2011 ), which may have been the result of the reduced circulating insulin
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, including GFAP and S100 protein ( Hazlerigg et al . 2001 ). The PT has a distinct developmental origin from the rest of the pituitary gland, involving the bHLH transcription factor hairy enhancer of split ( HES1 ) as a PT-specific differentiating factor
The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing, China
National Clinical Research Center for Cardiovascular Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, China
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The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing, China
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The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing, China
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Introduction Hypercholesterolemia is an independent risk factor for cardiovascular disease ( Trinder et al. 2020 ). The global burden of dyslipidemia has increased over the past 30 years ( Pirillo et al. 2021 ). Evidence from genetic
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. 5 B). Figure 5 The NF-κB transcription factor p65 is recruited to the Hsd11b1 P1 promoter upon stimulation by TNFα. (A) RNA isolated from C2C12 myotubes was assessed for the expression of Hsd11b1 promoter 1 (P1), promoter 2 (P2), total and GAPDH