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Jin-Ran Chen Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Oxana P Lazarenko Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Haijun Zhao Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Alexander W Alund Interdisciplinary Biomedical Sciences , University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Kartik Shankar Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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determining the effect of obesity on early postnatal life bone quantity ( Streeter et al . 2013 , Kâ et al . 2013 ), feeding a HFD rich in saturated fat and cholesterol to experimental rodents has consistently been shown to inhibit bone formation ( Parhami

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Thomas Funck-Brentano Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Karin H Nilsson Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Robert Brommage Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Petra Henning Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Ulf H Lerner Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Antti Koskela Unit of Cancer Research and Translational Medicine, MRC Oulu and Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland

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Juha Tuukkanen Unit of Cancer Research and Translational Medicine, MRC Oulu and Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland

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Martine Cohen-Solal BIOSCAR UMRS 1132, Université Paris Diderot, Sorbonne Paris Cité, INSERM, Paris, France

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Sofia Movérare-Skrtic Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Claes Ohlsson Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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, Immunodiagostic Systems) were analyzed as a marker of bone formation. Primary bone cell cultures Primary murine osteoblasts and osteoclasts were cultured in complete α-MEM medium (MEM alpha medium (Gibco) supplemented with 10% heat-inactivated FBS (Merck

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Vikte Lionikaite Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Karin L Gustafsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Anna Westerlund Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Sara H Windahl Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Antti Koskela Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, Oulu, Finland

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Juha Tuukkanen Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, Oulu, Finland

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Helena Johansson Institute for Health and Aging, Catholic University of Australia, Melbourne, Australia

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Claes Ohlsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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H Herschel Conaway Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Petra Henning Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Ulf H Lerner Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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et al. 2016 ), very few studies have analyzed effects of vitamin A on bone formation in vivo . Kneissel et al. and Lind et al. found that hypervitaminosis A decreased mineralizing surfaces on the periosteal side of cortical bone in the femur and

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J Jeyabalan
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M Shah
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B Viollet Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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J P Roux Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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P Chavassieux Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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M Korbonits Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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C Chenu
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ovariectomised or sham operated. Tibiae were collected from these mice 6 weeks after OVX for micro-computed tomography (micro-CT) analysis. For measurement of dynamic bone formation parameters, mice in this study were i.p. injected with calcein (Sigma

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David E Maridas Maine Medical Center Research Institute, Scarborough, Maine, USA

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Victoria E DeMambro Maine Medical Center Research Institute, Scarborough, Maine, USA

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Phuong T Le Maine Medical Center Research Institute, Scarborough, Maine, USA

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Kenichi Nagano Harvard School of Dental Medicine, Boston, Massachusetts, USA

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Roland Baron Harvard School of Dental Medicine, Boston, Massachusetts, USA

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Subburaman Mohan VA Loma Linda Healthcare System, Loma Linda, California, USA

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Clifford J Rosen Maine Medical Center Research Institute, Scarborough, Maine, USA

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conflicting results. Indeed, systemic injections of IGFBP-4 in mice induced bone formation, whereas local injections inhibited IGF actions on the bone ( Miyakoshi et al . 2001 ). Furthermore, overexpression of IGFBP-4 in bone resulted in a decrease in bone

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Claes Ohlsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Petra Henning Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Karin H Nilsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Jianyao Wu Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Karin L Gustafsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Klara Sjögren Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Anna Törnqvist Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Antti Koskela Department of Anatomy and Cell Biology, Institute of Cancer Research and Translational Medicine, Faculty of Medicine, University of Oulu, Oulu, Finland

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Fu-Ping Zhang Research Centre for Integrative Physiology and Pharmacology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland

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Marie K Lagerquist Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Matti Poutanen Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden
Research Centre for Integrative Physiology and Pharmacology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland

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Juha Tuukkanen Department of Anatomy and Cell Biology, Institute of Cancer Research and Translational Medicine, Faculty of Medicine, University of Oulu, Oulu, Finland

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Ulf H Lerner Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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Sofia Movérare-Skrtic Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, Gothenburg, Sweden

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osteoclast progenitor cells and by enhancing Opg expression in osteoblasts ( Moverare-Skrtic et al . 2014 ). In addition, it has been described that WNT16 may also regulate periosteal bone formation rate ( Wergedal et al . 2015 ). Although very

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Bernard Freudenthal Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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John Logan Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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Sanger Institute Mouse Pipelines Mouse Pipelines, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

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Peter I Croucher Garvan Institute of Medical Research, Sydney, New South Wales, Australia

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Graham R Williams Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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J H Duncan Bassett Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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Mechanism Reference Reduced bone mass Osteoporosis-
pseudoglioma syndrome (OPPGS) Reduced bone mass and blindness LRP5 Loss-of-function mutations disrupt Wnt signalling and reduce osteoblastic bone formation. ( Gong et al . 2001

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Louise Grahnemo Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Caroline Jochems Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Annica Andersson Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Cecilia Engdahl Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Claes Ohlsson Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Ulrika Islander Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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Hans Carlsten Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden

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. Serum levels of osteocalcin, a marker of bone formation, were determined with a Mouse Osteocalcin IRMA kit (Immutopics, Inc., San Clemente, CA, USA). The detection limits for CTX1 and osteocalcin were 6 and 0.1 ng/ml respectively. Bone strength

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K A Staines
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A S Pollard
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I M McGonnell
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C Farquharson Comparative Biomedical Sciences, Roslin Institute and R(D)SVS, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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A A Pitsillides
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. 1987 ). Interference with the maturational progression of growth plate chondrocytes leads to abnormal cartilage formation and ossification and modifications in the rates of bone formation, for example, in rickets and dwarfism. Details of the many

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K L Gustafsson Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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K H Nilsson Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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H H Farman Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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A Andersson Center for Bone and Arthritis Research, Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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V Lionikaite Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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P Henning Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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J Wu Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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S H Windahl Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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U Islander Center for Bone and Arthritis Research, Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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S Movérare-Skrtic Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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K Sjögren Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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H Carlsten Center for Bone and Arthritis Research, Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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J-Å Gustafsson Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, Texas, USA

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C Ohlsson Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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M K Lagerquist Center for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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fragments were assessed using an ELISA RatLaps kit (CTX-I, Immunodiagostic Systems, Copenhagen, Denmark). Serum levels of procollagen type I N propeptide (P1NP, Immunodiagostic Systems) were analyzed as a marker of bone formation. Statistical analyses

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