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Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
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Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
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Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
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Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
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determining the effect of obesity on early postnatal life bone quantity ( Streeter et al . 2013 , Kâ et al . 2013 ), feeding a HFD rich in saturated fat and cholesterol to experimental rodents has consistently been shown to inhibit bone formation ( Parhami
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, Immunodiagostic Systems) were analyzed as a marker of bone formation. Primary bone cell cultures Primary murine osteoblasts and osteoclasts were cultured in complete α-MEM medium (MEM alpha medium (Gibco) supplemented with 10% heat-inactivated FBS (Merck
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et al. 2016 ), very few studies have analyzed effects of vitamin A on bone formation in vivo . Kneissel et al. and Lind et al. found that hypervitaminosis A decreased mineralizing surfaces on the periosteal side of cortical bone in the femur and
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Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
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ovariectomised or sham operated. Tibiae were collected from these mice 6 weeks after OVX for micro-computed tomography (micro-CT) analysis. For measurement of dynamic bone formation parameters, mice in this study were i.p. injected with calcein (Sigma
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conflicting results. Indeed, systemic injections of IGFBP-4 in mice induced bone formation, whereas local injections inhibited IGF actions on the bone ( Miyakoshi et al . 2001 ). Furthermore, overexpression of IGFBP-4 in bone resulted in a decrease in bone
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Research Centre for Integrative Physiology and Pharmacology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland
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osteoclast progenitor cells and by enhancing Opg expression in osteoblasts ( Moverare-Skrtic et al . 2014 ). In addition, it has been described that WNT16 may also regulate periosteal bone formation rate ( Wergedal et al . 2015 ). Although very
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Mechanism Reference Reduced bone mass Osteoporosis- pseudoglioma syndrome (OPPGS) Reduced bone mass and blindness LRP5 Loss-of-function mutations disrupt Wnt signalling and reduce osteoblastic bone formation. ( Gong et al . 2001
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Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
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Departments of Rheumatology and Inflammation Research, Internal Medicine and Clinical Nutrition, Laboratory of Tumor Immunology and Biology, Centre for Bone and Arthritis Research, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Box 480, Gothenburg 405 30, Sweden
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. Serum levels of osteocalcin, a marker of bone formation, were determined with a Mouse Osteocalcin IRMA kit (Immutopics, Inc., San Clemente, CA, USA). The detection limits for CTX1 and osteocalcin were 6 and 0.1 ng/ml respectively. Bone strength
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. 1987 ). Interference with the maturational progression of growth plate chondrocytes leads to abnormal cartilage formation and ossification and modifications in the rates of bone formation, for example, in rickets and dwarfism. Details of the many
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fragments were assessed using an ELISA RatLaps kit (CTX-I, Immunodiagostic Systems, Copenhagen, Denmark). Serum levels of procollagen type I N propeptide (P1NP, Immunodiagostic Systems) were analyzed as a marker of bone formation. Statistical analyses