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Dipartimento Pediatrico Universitario Ospedaliero ‘Bambino Gesù’ Children’s Hospital – Tor Vergata University, Rome, Italy
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post-partum (d pp ), pups were weaned onto standard rat chow diet (Mucedola S.R.L., Milano, Italy), separated from dams and placed in groups of three to five, with the males and females separated. For the purpose of this study, only male pups were
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evidence for the existence of a pituitary-independent thyroid clock in the rat by demonstrating 24-h antiphase oscillations for the transcripts of the canonical clock genes Per1 and Bmal1 and by visualising a daily cytoplasmatic–nuclear shuttling of PER
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Introduction Spermatogenesis is a complex process that culminates in the production and release of step 19 spermatids in the rat, and it involves germ cell development, germ cell adhesion, and germ cell migration ( de Kretser & Kerr 1988 , O
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-responsive Wnt gene(s) involved in stromal cell proliferation. Wnt5a expression increased in progesterone-pretreated rat uteri and the protein localized to the presumptive decidual cells. Stromal cell lines stimulated with progesterone and FGF exhibited an
Department of Child Health, University of Arizona College of Medicine, Phoenix, Arizona, USA
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Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
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). We used these clinical observations to inform our translational research investigating GH-axis disruption following diffuse TBI in the juvenile rat. In the current study, we used postnatal day 17 rats, which model the late infantile/toddler period
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SYBR assay could be successfully developed to quantify total mouse and rat UCN3 expression, a mouse UCN3 Taqman assay (Applied Biosystems Mm00453206-s1) was performed to demonstrate relative expression of mUCN3 in muscles vs a dilution series of pCR
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sequence of the GC response elements, presence of coactivators or corepressors, and on post-translational modifications, such as phosphorylation ( Weigel & Moore 2007 ). The rat GR is phosphorylated at threonine 171 (T171), serine 224 (S224), serine 232 (S
Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
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Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
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Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
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Department of Veterinary Basic Sciences, Diabetes and Obesity Research Program, Faculty of Medicine, Department of Medicine, Faculty of Medicine, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
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silencing in rat TCMs in vivo IVE of EH114-GW-APPL1 was used to over-express APPL1 in the right TCMs of cohorts of chow-fed young adult rats, with EH114-EGFP administered to the left TCMs as paired control. After 1 week, immunoblotting of muscle lysates
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present in rat brain ( MacKenzie et al . 2000 a ). These studies showed expression of all these genes to occur in areas throughout the brain, with expression strongest within the hippocampus and dentate gyrus as well as the cerebellar granule layer and
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. 2009 , Otto et al . 2008 ). In addition, GPR30 is expressed in oxytocin (OXT) neurones of the rat hypothalamic paraventricular nucleus (PVH) and supraoptic nucleus (SON), implicating a role for GPR30 in the fast, non-genomic actions of E 2 on OXT