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Anne-Marie O'Carroll Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Gillian M Howell Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Emma M Roberts Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Stephen J Lolait Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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and stressful conditions ( Bale & Vale 2004 ). It rapidly stimulates adrenocorticotropin (ACTH) secretion by binding to the CRHR1 on pituitary corticotropes. This action is potentiated by the neurohypophyseal hormone arginine vasopressin (AVP) that has

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Michael J F Newson Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Emma M Roberts Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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George R Pope Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Stephen J Lolait Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Anne-Marie O'Carroll Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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nucleus is comprised of two major neurosecretory components – the mPVN and pPVN subdivisions. Neurones originating from the more medially situated pPVN (where vasopressin (AVP) is co-localised in a proportion of corticotrophin-releasing factor (CRF

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Emma M Roberts
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Michael J F Newson
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George R Pope
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Rainer Landgraf Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, Max Planck Institute of Psychiatry, University of Bristol, Bristol BS1 3NY, UK

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Stephen J Lolait
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Anne-Marie O'Carroll
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homeostasis is the hypothalamic PVN. Arginine vasopressin (AVP), a major neurohypophysial hormone within the HNS, is synthesised in magnocellular neurones of the hypothalamic PVN and SON ( Burbach et al . 2001 ) that project through the median eminence to the

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Georgina G J Hazell
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Song T Yao
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James A Roper
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Eric R Prossnitz LINE, University of New Mexico, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Anne-Marie O'Carroll
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Stephen J Lolait
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release ( Wang et al . 1995 , Revankar et al . 2005 ). Whether or not GPR30 is expressed in vasopressin (AVP) neurones remains unclear as one study found co-expression ( Brailoiu et al . 2007 ) while another did not ( Sakamoto et al . 2007 ), both

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Ying Sze Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK
The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, UK

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Joana Fernandes The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, UK

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Zofia M Kołodziejczyk Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

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Paula J Brunton Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK
The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, UK
Zhejiang University-University of Edinburgh Institute, International Campus, Haining, Zhejiang, P.R. China

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, using oligonucleotide probes (Supplementary Table 1A) for arginine vasopressin ( Avp ) and pro-opiomelanocortin ( Pomc ) or riboprobes (Supplementary Table 1B) for corticotropin-releasing hormone ( Crh ), glucocorticoid receptor ( Nr3c1

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M J F Newson Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), School of Clinical Sciences, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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G R Pope Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), School of Clinical Sciences, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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E M Roberts Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), School of Clinical Sciences, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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S J Lolait Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), School of Clinical Sciences, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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A-M O'Carroll Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), School of Clinical Sciences, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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GL 1980 Rat vasopressin response to insulin-induced hypoglycemia . Endocrinology 107 1975 – 1979 . ( doi:10.1210/endo-107-6-1975 ) Beishuizen A Thijs LG 2003 Endotoxin and the hypothalamo–pituitary–adrenal (HPA) axis . Journal of

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Dawn E W Livingstone Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Emma M Di Rollo Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Chenjing Yang Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Lucy E Codrington Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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John A Mathews Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Madina Kara Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Katherine A Hughes Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Christopher J Kenyon Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Brian R Walker Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Ruth Andrew Endocrinology, Queen's Medical Research Institute, University and British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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b1 ) tctacaaatgaagagttcagaccag gccccagtgacaatcacttt 1 Glucocorticoid receptor ( Nr3c1 ) gacgtgtggaagctgtaaagt catttcttccagcacaaaggt 56 Arginine vasopressin ( Avp ) gctgccaggaggagaactac aaaaccgtcgtggcactc 84 TATA box-binding protein ( Tbp

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David O'Regan Endocrinology Unit, Queen's Medical Research Institute, Centre for Cardiovascular Science, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Christopher J Kenyon Endocrinology Unit, Queen's Medical Research Institute, Centre for Cardiovascular Science, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Jonathan R Seckl Endocrinology Unit, Queen's Medical Research Institute, Centre for Cardiovascular Science, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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Megan C Holmes Endocrinology Unit, Queen's Medical Research Institute, Centre for Cardiovascular Science, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

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. Vascular responses to noradrenaline, vasopressin and potassium chloride Pairs of rats (one VEH and one DEX) were anaesthetised with sodium pentobarbitone, and the mesenteric vasculature was catheterised and perfused with Krebs bicarbonate Ringer

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Saeed Alshahrani Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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Mohammed Mashari Almutairi Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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Shams Kursan Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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Eduardo Dias-Junior Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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Mohamed Mahmoud Almiahuob Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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Lydia Aguilar-Bryan Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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Mauricio Di Fulvio Department of Pharmacology and Toxicology, Pacific Northwest Diabetes Research Institute, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, 216 HSB, Dayton, Ohio 45435, USA

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including insulin- ( Alshahrani et al . 2012 ), oxytocin- and vasopressin-secreting ones ( Hindmarch et al . 2006 , Konopacka et al . 2015 ) are compatible with the concept that NKCC2 cannot fully ‘compensate’ for NKCC1 functional absence. Recent reports

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Ryun S Ahn
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Jee H Choi Graduate School of Integrative Medicine, Hormone Research Center, Department of Obstetrics and Gynecology, Department of Family Medicine, Department of Life sciences, Research Institute of Integrative Medicine, CHA Medical University, Yuksam‐dong 605, Kangnamgu, Seoul 135-907, Republic of Korea

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Bum C Choi Graduate School of Integrative Medicine, Hormone Research Center, Department of Obstetrics and Gynecology, Department of Family Medicine, Department of Life sciences, Research Institute of Integrative Medicine, CHA Medical University, Yuksam‐dong 605, Kangnamgu, Seoul 135-907, Republic of Korea

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Jung H Kim Graduate School of Integrative Medicine, Hormone Research Center, Department of Obstetrics and Gynecology, Department of Family Medicine, Department of Life sciences, Research Institute of Integrative Medicine, CHA Medical University, Yuksam‐dong 605, Kangnamgu, Seoul 135-907, Republic of Korea

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Sung H Lee Graduate School of Integrative Medicine, Hormone Research Center, Department of Obstetrics and Gynecology, Department of Family Medicine, Department of Life sciences, Research Institute of Integrative Medicine, CHA Medical University, Yuksam‐dong 605, Kangnamgu, Seoul 135-907, Republic of Korea

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Simon S Sung Graduate School of Integrative Medicine, Hormone Research Center, Department of Obstetrics and Gynecology, Department of Family Medicine, Department of Life sciences, Research Institute of Integrative Medicine, CHA Medical University, Yuksam‐dong 605, Kangnamgu, Seoul 135-907, Republic of Korea

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, it is well documented that the SCN communicates with neuroendocrine neurons via neurotransmitters, such as arginine vasopressin (AVP) and vasoactive intestinal polypeptide (VIP; Buijs et al . 2003 ). The rhythmicity of AVP release from SCN terminals

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