Search Results
You are looking at 1 - 3 of 3 items for
- Author: Yoichi Ueta x
- Refine by access: Content accessible to me x
Department of Physiology, Laboratory of Physiology, Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Search for other papers by Hiroki Otsubo in
Google Scholar
PubMed
Search for other papers by Susumu Hyodo in
Google Scholar
PubMed
Search for other papers by Hirofumi Hashimoto in
Google Scholar
PubMed
Search for other papers by Makoto Kawasaki in
Google Scholar
PubMed
Search for other papers by Hitoshi Suzuki in
Google Scholar
PubMed
Search for other papers by Takeshi Saito in
Google Scholar
PubMed
Search for other papers by Toyoaki Ohbuchi in
Google Scholar
PubMed
Search for other papers by Toru Yokoyama in
Google Scholar
PubMed
Search for other papers by Hiroaki Fujihara in
Google Scholar
PubMed
Search for other papers by Tetsuro Matsumoto in
Google Scholar
PubMed
Search for other papers by Yoshio Takei in
Google Scholar
PubMed
Search for other papers by Yoichi Ueta in
Google Scholar
PubMed
We examined the effects of i.c.v. administration of adrenomedullin 5 (AM5) on the brain of conscious rats. We used porcine AM5 in the present study because rat AM5 has not been detected. We observed Fos-like immunoreactivity (LI) in the hypothalamus and brainstem of conscious rats after i.c.v. administration of AM5 (2 nmol/rat). Fos-LI, measured at 90 min post-AM5 injection, was observed in various brain areas, including the supraoptic (SON) and the paraventricular nuclei (PVN). Dual immunostaining for Fos/oxytocin (OXT) and Fos/arginine vasopressin (AVP) revealed that OXT-LI neurones predominantly colocalized Fos-LI compared with AVP-LI neurones in the SON and the PVN. Plasma OXT levels were significantly increased 5 min after i.c.v. administration of AM5 (1 nmol/rat) compared with vehicle and remained elevated in samples taken at 15 and 30 min without changes in plasma AVP levels at any time. In situ hybridization histochemistry showed that i.c.v. administration of AM5 (0.2, 1 and 2 nmol/rat) caused a marked induction of the expression of the c-fos gene in the SON and the PVN. This induction was significantly but not completely reduced by pretreatment with both the calcitonin gene-related peptide (CGRP) antagonist CGRP-(8–37; 3 nmol/rat) and the AM receptor antagonist AM-(22–52; 27 nmol/rat). Although porcine AM5 has not been detected yet in the brain, these results suggest that centrally administered porcine AM5 may activate OXT-secreting neurosecretory cells in the hypothalamus partly through AM/CGRP receptors and elicit secretion of OXT into the systemic circulation in conscious rats.
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Makoto Kawasaki in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Tatsushi Onaka in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Masamitsu Nakazato in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Jun Saito in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Takashi Mera in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Hirofumi Hashimoto in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Hiroaki Fujihara in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Nobukazu Okimoto in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Hideo Ohnishi in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Toshitaka Nakamura in
Google Scholar
PubMed
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
Search for other papers by Yoichi Ueta in
Google Scholar
PubMed
We examined the effects of i.c.v. administration of neuro-peptide W-30 (NPW30) on plasma arginine vasopressin (AVP) and plasma oxytocin (OXT) using RIA. The induction of c-fos mRNA, AVP heteronuclear (hn)RNA, and c-Fos protein (Fos) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of rats were also investigated using in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA, and immunohistochemistry for Fos. Both plasma AVP and OXT were significantly increased at 5 and 15 min after i.c.v. administration of NPW30 (2.8 nmol/rat). In situ hybridization histochemistry revealed that the induction of c-fos mRNA and AVP hnRNA in the SON and PVN were significantly increased 15, 30, and 60 min after i.c.v. administration of NPW30 (1.4 nmol/rat). Dual immunostaining for Fos/AVP and Fos/OXT revealed that both AVP-like immunoreactive (LI) cells and OXT-LI cells exhibited nuclear Fos-LI in the SON and PVN, 90 min after i.c.v. administration of NPW30 (2.8 nmol/rat). These results suggest that central NPW30 may be involved in the regulation of secretion of AVP and OXT in the magnocellular neurosecretory cells in the SON and PVN.
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
Search for other papers by Akiko Katoh in
Google Scholar
PubMed
Search for other papers by Hiroaki Fujihara in
Google Scholar
PubMed
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
Search for other papers by Toyoaki Ohbuchi in
Google Scholar
PubMed
Search for other papers by Tatsushi Onaka in
Google Scholar
PubMed
Search for other papers by W Scott Young III in
Google Scholar
PubMed
Search for other papers by Govindan Dayanithi in
Google Scholar
PubMed
Search for other papers by Yuka Yamasaki in
Google Scholar
PubMed
Search for other papers by Mitsuhiro Kawata in
Google Scholar
PubMed
Search for other papers by Hitoshi Suzuki in
Google Scholar
PubMed
Search for other papers by Hiroki Otsubo in
Google Scholar
PubMed
Search for other papers by Hideaki Suzuki in
Google Scholar
PubMed
Search for other papers by David Murphy in
Google Scholar
PubMed
Search for other papers by Yoichi Ueta in
Google Scholar
PubMed
We have generated rats bearing an oxytocin (OXT)-enhanced cyan fluorescent protein (eCFP) fusion transgene designed from a murine construct previously shown to be faithfully expressed in transgenic mice. In situ hybridisation histochemistry revealed that the Oxt–eCfp fusion gene was expressed in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) in these rats. The fluorescence emanating from eCFP was observed only in the SON, the PVN, the internal layer of the median eminence and the posterior pituitary (PP). In in vitro preparations, freshly dissociated cells from the SON and axon terminals showed clear eCFP fluorescence. Immunohistochemistry for OXT and arginine vasopressin (AVP) revealed that the eCFP fluorescence co-localises with OXT immunofluorescence, but not with AVP immunofluorescence in the SON and the PVN. Although the expression levels of the Oxt–eCfp fusion gene in the SON and the PVN showed a wide range of variations in transgenic rats, eCFP fluorescence was markedly increased in the SON and the PVN, but decreased in the PP after chronic salt loading. The expression of the Oxt gene was significantly increased in the SON and the PVN after chronic salt loading in both non-transgenic and transgenic rats. Compared with wild-type animals, euhydrated and salt-loaded male and female transgenic rats showed no significant differences in plasma osmolality, sodium concentration and OXT and AVP levels, suggesting that the fusion gene expression did not disturb any physiological processes. These results suggest that our new transgenic rats are a valuable new tool to identify OXT-producing neurones and their terminals.
