Search Results
Department of Molecules and Circuits, CNRS UMR 9197, Molecular Neuroendocrinology of Food Intake, Paris-Saclay Institute of Neuroscience, Orsay, France
Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Search for other papers by Jie Miao in
Google Scholar
PubMed
Department of Molecules and Circuits, CNRS UMR 9197, Molecular Neuroendocrinology of Food Intake, Paris-Saclay Institute of Neuroscience, Orsay, France
Search for other papers by Yacir Benomar in
Google Scholar
PubMed
Search for other papers by Sarah Al Rifai in
Google Scholar
PubMed
Department of Molecules and Circuits, CNRS UMR 9197, Molecular Neuroendocrinology of Food Intake, Paris-Saclay Institute of Neuroscience, Orsay, France
Search for other papers by Ghislaine Poizat in
Google Scholar
PubMed
Department of Molecules and Circuits, CNRS UMR 9197, Molecular Neuroendocrinology of Food Intake, Paris-Saclay Institute of Neuroscience, Orsay, France
Search for other papers by Laure Riffault in
Google Scholar
PubMed
Department of Molecules and Circuits, CNRS UMR 9197, Molecular Neuroendocrinology of Food Intake, Paris-Saclay Institute of Neuroscience, Orsay, France
Search for other papers by Delphine Crépin in
Google Scholar
PubMed
Department of Molecules and Circuits, CNRS UMR 9197, Molecular Neuroendocrinology of Food Intake, Paris-Saclay Institute of Neuroscience, Orsay, France
Search for other papers by Mohammed Taouis in
Google Scholar
PubMed
all antibodies were from Cell Signaling Technology: LC3A/B, ATG7, P62, phospho (p)-AKT (ser473), AKT, p-mTOR (ser2448), mTOR, AMPK, p-AMPK, β-tubulin and TLR4. Blots were then incubated with secondary mouse or rabbit antibodies coupled to horseradish
Search for other papers by Véronique Serre-Beinier in
Google Scholar
PubMed
Search for other papers by Christian Toso in
Google Scholar
PubMed
Search for other papers by Philippe Morel in
Google Scholar
PubMed
Search for other papers by Carmen Gonelle-Gispert in
Google Scholar
PubMed
Search for other papers by Christelle Veyrat-Durebex in
Google Scholar
PubMed
Search for other papers by Françoise Rohner-Jeanrenaud in
Google Scholar
PubMed
Search for other papers by Thierry Calandra in
Google Scholar
PubMed
Search for other papers by Thierry Roger in
Google Scholar
PubMed
Search for other papers by Richard W James in
Google Scholar
PubMed
Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery
Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery
Search for other papers by Xavier Montet in
Google Scholar
PubMed
Search for other papers by Léo Bühler in
Google Scholar
PubMed
Search for other papers by Domenico Bosco in
Google Scholar
PubMed
Search for other papers by Thierry Berney in
Google Scholar
PubMed
for 1 h at room temperature in a 10 mmol/l Tris–HCl buffer (pH 7.4) containing 150 mmol/l NaCl, 0.1% (v/v) Tween-20, and 5% BSA, and then incubated overnight at 4 °C with antibody. Antibodies against AKT and phospho-AKT (Ser 473 ) were obtained from
Search for other papers by Yoshimitsu Kuwabara in
Google Scholar
PubMed
Search for other papers by Akira Katayama in
Google Scholar
PubMed
Search for other papers by Ryoko Tomiyama in
Google Scholar
PubMed
Search for other papers by Hu Piao in
Google Scholar
PubMed
Departments of Obstetrics and Gynecology, Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan
Search for other papers by Sachiko Kurihara in
Google Scholar
PubMed
Search for other papers by Shuichi Ono in
Google Scholar
PubMed
Search for other papers by Katsuya Mine in
Google Scholar
PubMed
Search for other papers by Shigeo Akira in
Google Scholar
PubMed
Search for other papers by Hideo Orimo in
Google Scholar
PubMed
Search for other papers by Toshiyuki Takeshita in
Google Scholar
PubMed
(Cayman Chemical Company, Ann Arbor, MI, USA; cat #582601) according to the manufacturer's instructions. Western blotting analysis To analyze AKT phosphorylation, 10 μg of control or OPN-treated cell lysate was separated on a 4–20% SDS–polyacrylamide gel
Graduate School of Peking Union Medical College, Institute of Clinical Medical Sciences, Department of Cell Physiology and Metabolism, Beijing 100730, People's Republic of China
Search for other papers by Bo Qian in
Google Scholar
PubMed
Search for other papers by Haiyan Wang in
Google Scholar
PubMed
Search for other papers by Xiuli Men in
Google Scholar
PubMed
Search for other papers by Wenjian Zhang in
Google Scholar
PubMed
Search for other papers by Hanqing Cai in
Google Scholar
PubMed
Search for other papers by Shiqing Xu in
Google Scholar
PubMed
Search for other papers by Yaping Xu in
Google Scholar
PubMed
Search for other papers by Liya Ye in
Google Scholar
PubMed
Search for other papers by Claes B Wollheim in
Google Scholar
PubMed
Search for other papers by Jinning Lou in
Google Scholar
PubMed
attracted interest in diabetes research as it has emerged as an endogenous inhibitor of Akt (PKB), which plays a key role in insulin signaling ( Du et al . 2003 ). In addition, the mRNA levels of TRIB3 are elevated in the liver of db/db mice ( Matsushima
Search for other papers by Ana Carolina Ronda in
Google Scholar
PubMed
Search for other papers by Andrea Vasconsuelo in
Google Scholar
PubMed
Search for other papers by Ricardo Boland in
Google Scholar
PubMed
exerts antiapoptotic effects in the C2C12 muscle cell line upon exposure to H 2 O 2 or etoposide involving the phosphatidylinositol 3-kinase (PI3K)/AKT/BAD pathway ( Vasconsuelo et al . 2008 ). Also, the antiapoptotic action of the steroid hormone has
Search for other papers by Graziela R Stoppa in
Google Scholar
PubMed
Search for other papers by Maristela Cesquini in
Google Scholar
PubMed
Search for other papers by Erika A Roman in
Google Scholar
PubMed
Search for other papers by Patrícia O Prada in
Google Scholar
PubMed
Search for other papers by Adriana S Torsoni in
Google Scholar
PubMed
Search for other papers by Talita Romanatto in
Google Scholar
PubMed
Search for other papers by Mario J Saad in
Google Scholar
PubMed
Search for other papers by Licio A Velloso in
Google Scholar
PubMed
Search for other papers by Marcio A Torsoni in
Google Scholar
PubMed
(endovenous, EV). Protein expression was evaluated through immunoblotting with anti total-IR, anti total-IRS1, anti total-IRS2, and anti total-AKT. Protein expression did not change in either tissue (epididymal fat pad and skeletal muscle) after insulin
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Search for other papers by Michelle Keramidas in
Google Scholar
PubMed
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Search for other papers by Caroline Faudot in
Google Scholar
PubMed
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Search for other papers by Agnès Cibiel in
Google Scholar
PubMed
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Search for other papers by Jean-Jacques Feige in
Google Scholar
PubMed
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Institut National de la Santé et de la Recherche Médicale, Commissariat à l'Energie Atomique, Université Joseph Fourier, Unité 878, Grenoble, France
Search for other papers by Michaël Thomas in
Google Scholar
PubMed
hydrochloride), and 1 mM sodium orthovanadate) and separated by SDS-PAGE. After electrophoretic transfer onto a nitrocellulose membrane, the phosphorylated forms of ERK1/2 and Akt were analyzed by western blotting using phosphorylation site-specific antibodies
Search for other papers by Victor Wong in
Google Scholar
PubMed
Search for other papers by Linda Szeto in
Google Scholar
PubMed
Search for other papers by Kristine Uffelman in
Google Scholar
PubMed
Search for other papers by I George Fantus in
Google Scholar
PubMed
Search for other papers by Gary F Lewis in
Google Scholar
PubMed
Biotechnology, Inc. (Santa Cruz, CA, USA). Agarose-conjugated anti-insulin receptor substrate-1 (anti-IRS-1) and anti-p85 subunit of phosphatidylinositol 3-kinase (PI3K) antibodies were purchased from Upstate Biotechnology, Inc. (Lake Placid, NY, USA). Anti-AKT
Department of Physiology, Medical University of Bialystok, Bialystok, Poland
Search for other papers by Piotr Zabielski in
Google Scholar
PubMed
Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, Bialystok, Poland
Search for other papers by Marta Chacinska in
Google Scholar
PubMed
Department of Perinatology, Medical University of Bialystok, Bialystok, Poland
Search for other papers by Karol Charkiewicz in
Google Scholar
PubMed
Search for other papers by Marcin Baranowski in
Google Scholar
PubMed
Search for other papers by Jan Gorski in
Google Scholar
PubMed
Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, Bialystok, Poland
Search for other papers by Agnieszka U Blachnio-Zabielska in
Google Scholar
PubMed
isoenzymes ( Itani et al . 2002 ), whereas accumulation of Cer can inhibit the insulin signaling pathway via protein phosphatase 2A (PPA2)-mediated inhibition of Akt/PKB ( Schmitz-Peiffer et al . 1999 ) ( Fig. 1 ). Figure 1 Mechanism of FFA
Search for other papers by Jonathan M Mudry in
Google Scholar
PubMed
Search for other papers by Julie Massart in
Google Scholar
PubMed
Search for other papers by Ferenc L M Szekeres in
Google Scholar
PubMed
Section for Integrative Physiology, Section for Integrative Physiology, Department of Molecular Medicine and Surgery
Search for other papers by Anna Krook in
Google Scholar
PubMed
641 (p-GS) (#3891), ACC (#3676), ACC phosphorylated on serine 79 (p-ACC) (#3661), AKT (or protein kinase B (PKB)) (#9272), and its phosphorylated form on serine 473 (p-AKT) (#9271) were purchased from Cell Signaling (Danvers, MA, USA), GAPDH (#sc