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Maayan Vatarescu The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel

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Sapir Bechor The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel

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Yulia Haim The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel

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Tal Pecht The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel

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Tanya Tarnovscki The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

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Noa Slutsky The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

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Ori Nov The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

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Hagit Shapiro The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

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Avishai Shemesh The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University, Beer-Sheva, Israel

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Angel Porgador The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University, Beer-Sheva, Israel

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Nava Bashan The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

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Assaf Rudich The Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
The National Institute of Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel

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inflammation (particularly macrophage infiltration) characterizes obese persons who develop insulin resistance ( Harman-Boehm et al . 2007 ), but to a much lesser degree age, sex and BMI-matched obese persons who remain insulin sensitive ( Kloting et al

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-17-0007
Volume/Issue:
Volume 233: Issue 3
Open access
Xiaohui Wang
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Yuxia Chen
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Yan Wang
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Xiaoyan Zhu
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Yuanyuan Ma
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Shimin Zhang Department of Pathophysiology, American Registry of Pathology at Armed Forces Institute of Pathology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People's Republic of China

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Jian Lu
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cytokine production in macrophages ( Distelhorst 2002 , Frankfurt & Rosen 2004 ). Macrophages also play a key role in the immune and inflammatory process, mainly acting as antigen-processing/presenting cells and sources of inflammatory cytokines. Although

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-08-0340
Volume/Issue:
Volume 200: Issue 1
Free access
Michael P Corcoran Lipid Metabolism Laboratory, Vascular Biology Laboratory, Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA

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Mohsen Meydani Lipid Metabolism Laboratory, Vascular Biology Laboratory, Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA

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Alice H Lichtenstein Lipid Metabolism Laboratory, Vascular Biology Laboratory, Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA

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Ernst J Schaefer Lipid Metabolism Laboratory, Vascular Biology Laboratory, Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA

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Alice Dillard Lipid Metabolism Laboratory, Vascular Biology Laboratory, Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA

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Stefania Lamon-Fava Lipid Metabolism Laboratory, Vascular Biology Laboratory, Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA

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local inflammation ( Brown & Goldstein 1983 ). Recruited monocytes differentiate into macrophages and begin to take up oxidized lipoproteins, leading to the formation of foam cells. Foam cells are a primary component of early atheroma lesion formation

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-10-0057
Volume/Issue:
Volume 206: Issue 2
Free access
L E Nicol Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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W F Grant Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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S M Comstock Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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M L Nguyen Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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M S Smith Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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K L Grove Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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D L Marks Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute
Pediatrics, Division of Neurosciences, Pape Pediatric Research Institute

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(Arg1; Rh02826373_m1) M1- and M2-activated macrophages respectively from the same cDNA as mentioned earlier. Quantitative PCRs were run on an ABI 7300 (Applied Biosystems), using TaqMan universal PCR master mix TaqMan master mix in a 10 μl reaction

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-12-0424
Volume/Issue:
Volume 217: Issue 2
Free access
Hyo Youl Moon
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Parkyong Song BioSignal Network Laboratory, Division of Molecular and Life Sciences, Lee Gil Ya Cancer and Diabetes Institute, School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Engineering Building 104, 689-805 Ulsan, Republic of Korea

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Cheol Soo Choi BioSignal Network Laboratory, Division of Molecular and Life Sciences, Lee Gil Ya Cancer and Diabetes Institute, School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Engineering Building 104, 689-805 Ulsan, Republic of Korea

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Sung Ho Ryu BioSignal Network Laboratory, Division of Molecular and Life Sciences, Lee Gil Ya Cancer and Diabetes Institute, School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Engineering Building 104, 689-805 Ulsan, Republic of Korea

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Pann-Ghill Suh
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) ( Ruderman & Prentki 2004 ). Based on the requirement for AMPK to alleviate hepatic steatosis, there is growing demand to identify mediators of AMPK activation in response to dynamic physiological activity. Although macrophage migration inhibitory factor (MIF

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-13-0135
Volume/Issue:
Volume 218: Issue 3
Open access
Véronique Serre-Beinier Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Christian Toso Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Philippe Morel Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Carmen Gonelle-Gispert Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Christelle Veyrat-Durebex Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Françoise Rohner-Jeanrenaud Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Thierry Calandra Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Thierry Roger Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Richard W James Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Xavier Montet Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery
Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery
Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Léo Bühler Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Domenico Bosco Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Thierry Berney Surgical Research Unit, Department of Surgery, Laboratory of Metabolism, Infectious Diseases Service, Clinical Diabetes Unit, Radiology, Cell Physiology and Metabolism, Internal Medicine, Department of Surgery

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Introduction Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine involved in both innate and adaptive immunity. Originally described as a T-cell-derived product, MIF has now been shown to be released by numerous cell types and

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-09-0342
Volume/Issue:
Volume 206: Issue 3
Free access
Anne Q Reuwer Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Marco van Eijk Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Felicia M Houttuijn-Bloemendaal Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Chris M van der Loos Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Nike Claessen Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Peter Teeling Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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John J P Kastelein Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Jörg Hamann Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Vincent Goffin Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Jan H von der Thüsen Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Marcel Th B Twickler Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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Jan Aten Departments of, Vascular Medicine, Medical Biochemistry, Pathology, Experimental Immunology, INSERM, Department of Internal Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

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by inflammatory cell infiltration, accumulation of heterogeneous macrophage populations, T-cell activation, cytokine production, and cell death, is also an integral part of the atherosclerotic process ( Packard & Libby 2008 ). In fact, all stages of

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-10-0076
Volume/Issue:
Volume 208: Issue 2
Free access
Uniza W Khan Comparative Immunoendocrinology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India

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Umesh Rai Comparative Immunoendocrinology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India

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). In the last decade, there has been a groundswell of interest in understanding the interrelationship between Leydig cells and testicular macrophages ( Haider 2004 , Hutson 2006 ). Moreover, lymphocytes and low number of mast cells are also present

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-06-0225
Volume/Issue:
Volume 194: Issue 2
Free access
Kelly D McCall Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Dawn Holliday Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Eric Dickerson Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Brian Wallace Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Anthony L Schwartz Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Christopher Schwartz Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Christopher J Lewis Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Leonard D Kohn Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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Frank L Schwartz Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center
Department of Specialty Medicine, Appalachian Rural Health Institute, Edison Biotechnology Institute, Department of Biomedical Sciences, Biomedical Engineering Program, Interthyr Corporation, Diabetes Research Center

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adipocytes and associated inflammatory cells (macrophages) produce and release excessive amounts of biologically active ‘cytokines/adipokines’ (TNF-α, resistin, IL6, and PAI-1, etc.), which circulate via the portal and systemic vascular system to other

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-09-0370
Volume/Issue:
Volume 207: Issue 3
Free access
M Schmidt
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M Kreutz
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G Loffler
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J Scholmerich
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RH Straub
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Dehydroepiandrosterone (DHEA) is a ubiquitous adrenal hormone with immunomodulatory effects such as inhibition of the production of monokines. Whether DHEA itself or the downstream steroids are the immunomodulatory effector hormones in target cells is not known. In this study, we investigated the conversion of DHEA to downstream steroid hormones in target macrophages. Within 1 day of culture with radiolabeled DHEA, monocyte-derived macrophages converted DHEA to significant amounts of Delta5-derivatives such as 16OH-DHEA, 3beta, 17beta-androstenediol (A'diol), and 3beta,16alpha, 17beta-androstenetriol (A'triol). However, the production of Delta4-steroids (androstenedione (A'dione), testosterone (T), and 16OH-T) and estrogens (estrone, estradiol, and estriol) was relatively low. Further cultivation of macrophages for 5 days with radiolabeled DHEA resulted in a significant (P<0.05) increase of the molar amounts of A'triol (P=0.012), 16OH-T (P=0.008), and estriol (P=0.003). In contrast to monocyte-derived macrophages, monocytes did not express aromatase mRNA, which was demonstrated by RT-PCR (P<0.01). Furthermore, DHEA in macrophages significantly inhibited one of the downstream converting enzymes, the aromatase, which was not demonstrated in the presence of the typical macrophage activator, lipopolysaccharide (LPS) (P<0.01). In conclusion, conversion of DHEA to physiologically relevant amounts of Delta5- and Delta4-steroids and estrogens was demonstrated in monocyte-derived macrophages. The conversion depends on maturation of monocytes and local factors such as the presence of LPS. The conversion of DHEA leads to an increase of downstream effector hormones in target macrophages which may be an important factor for local immunomodulation.

Article Type:
Other
DOI:
https://doi.org/10.1677/joe.0.1640161
Volume/Issue:
Volume 164: Issue 2
Free access