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Johan Svensson
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Jon Kindblom Division of Endocrinology, Department of Oncology, Department of Physiology/Endocrinology, Laboratory for Experimental Medicine and Endocrinology, Department of Internal Medicine, Sahlgrenska Academy, Sahlgrenska University Hospital, Göteborg University, Gröna Stråket 8, SE-413 45 Göteborg, Sweden

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Ruijin Shao Division of Endocrinology, Department of Oncology, Department of Physiology/Endocrinology, Laboratory for Experimental Medicine and Endocrinology, Department of Internal Medicine, Sahlgrenska Academy, Sahlgrenska University Hospital, Göteborg University, Gröna Stråket 8, SE-413 45 Göteborg, Sweden

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Sofia Movérare-Skrtic
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Marie K Lagerquist
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Niklas Andersson
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Klara Sjögren
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Katrien Venken Division of Endocrinology, Department of Oncology, Department of Physiology/Endocrinology, Laboratory for Experimental Medicine and Endocrinology, Department of Internal Medicine, Sahlgrenska Academy, Sahlgrenska University Hospital, Göteborg University, Gröna Stråket 8, SE-413 45 Göteborg, Sweden

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Dirk Vanderschueren Division of Endocrinology, Department of Oncology, Department of Physiology/Endocrinology, Laboratory for Experimental Medicine and Endocrinology, Department of Internal Medicine, Sahlgrenska Academy, Sahlgrenska University Hospital, Göteborg University, Gröna Stråket 8, SE-413 45 Göteborg, Sweden

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John-Olov Jansson
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Olle Isaksson
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Claes Ohlsson
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Inducible gene targeting in mice . Science 269 1427 – 1429 . Lam J Leppert J Vemulapalli S Shvarts O Belldegrun A 2006 Secondary hormonal therapy for advanced prostate cancer . Journal of Urology 175 27 – 34 . Liu J Grinberg A

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J M P Pabona Department of Physiology and Biophysics, University of Arkansas for Medical Sciences and Arkansas Children's Nutrition Center, 1212 Marshall Street, Little Rock, Arkansas 72202, USA

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M C Velarde Department of Physiology and Biophysics, University of Arkansas for Medical Sciences and Arkansas Children's Nutrition Center, 1212 Marshall Street, Little Rock, Arkansas 72202, USA

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Z Zeng Department of Physiology and Biophysics, University of Arkansas for Medical Sciences and Arkansas Children's Nutrition Center, 1212 Marshall Street, Little Rock, Arkansas 72202, USA

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F A Simmen Department of Physiology and Biophysics, University of Arkansas for Medical Sciences and Arkansas Children's Nutrition Center, 1212 Marshall Street, Little Rock, Arkansas 72202, USA

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R C M Simmen Department of Physiology and Biophysics, University of Arkansas for Medical Sciences and Arkansas Children's Nutrition Center, 1212 Marshall Street, Little Rock, Arkansas 72202, USA

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binding protein by gene targeting technology . Molecular and Cellular Biology 23 2489 – 2500 . Mussi P Liao L Park S-E Ciana P Maggi A Katzenellenbogen BS Xu J O'Malley BW 2006 Haploinsufficiency of the corepressor of estrogen

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Shin Tsunekawa Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Naoki Yamamoto Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Katsura Tsukamoto Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Yuji Itoh Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Yukiko Kaneko Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Toshihide Kimura Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Yoh Ariyoshi Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Yoshitaka Miura Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Yutaka Oiso Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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Ichiro Niki Department of Endocrinology and Diabetes, School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
Central Research Laboratory, Sanwa Kagaku Kenkyusho Co. Ltd, 363 Shiosaki, Hokusei, Inabe, Mie 511-0406, Japan
Department of Pharmacology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan

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; Yu et al. 2002 ). When we standardized these values by their pancreatic insulin content at this age ( Tsunekawa et al. 2005 ), ≈4.9 times overexpression of calmodulin was achieved by the gene targeting. The overexpression rate is relatively not

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Johan Svensson Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Åsa Tivesten Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Klara Sjögren Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Olle Isaksson Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Göran Bergström Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Subburaman Mohan Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Johan Mölne Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Jörgen Isgaard Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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Claes Ohlsson Department of Internal Medicine, Sahlgrenska University Hospital, Gröna Stråket 8, SE-413 45 Göteborg, Sweden
Department of Clinical Physiology, Göteborg University, Göteborg, Sweden
Musculoskeletal Disease Center, Jerry L Pettis Memorial VA Medical Center, Loma Linda, California, USA
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden

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, Schwenk F, Aguet M & Rajewsky K 1995 Inducible gene targeting in mice. Science 269 1427 –1429. Landin-Wilhelmsen K , Wilhelmsen L, Lappas G, Rosén T, Lindstedt G, Lundberg P-A & Bengtsson B-Å 1994 Serum insulin

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Qinglei Li Laboratory of Mammalian Reproductive Biology and Genomics,
Departments of Animal Science
Physiology
Molecular Reproductive Endocrinology Laboratory, Michigan State University, East Lansing, Michigan 48824, USA

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Fermin Jimenez-Krassel Laboratory of Mammalian Reproductive Biology and Genomics,
Departments of Animal Science
Physiology
Molecular Reproductive Endocrinology Laboratory, Michigan State University, East Lansing, Michigan 48824, USA

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Anilkumar Bettegowda Laboratory of Mammalian Reproductive Biology and Genomics,
Departments of Animal Science
Physiology
Molecular Reproductive Endocrinology Laboratory, Michigan State University, East Lansing, Michigan 48824, USA

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James J Ireland Laboratory of Mammalian Reproductive Biology and Genomics,
Departments of Animal Science
Physiology
Molecular Reproductive Endocrinology Laboratory, Michigan State University, East Lansing, Michigan 48824, USA

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George W Smith Laboratory of Mammalian Reproductive Biology and Genomics,
Departments of Animal Science
Physiology
Molecular Reproductive Endocrinology Laboratory, Michigan State University, East Lansing, Michigan 48824, USA

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). Furthermore, a requirement of the progesterone receptor for ovulation in mice has been demonstrated using gene targeting technology ( Lydon et al. 1995 , 1996 ). Although data in the mouse support a potential role for progesterone-mediated regulation of a

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Yoko Fujiwara Department of Pharmacology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

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Masami Hiroyama Department of Pharmacology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

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Atsushi Sanbe Department of Pharmacology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

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Junji Yamauchi Department of Pharmacology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

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Gozoh Tsujimoto Department of Pharmacology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

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Akito Tanoue Department of Pharmacology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

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). Animals Male mice deficient in the V1b vasopressin receptor were generated by gene targeting as described previously ( Tanoue et al. 2004 ). Briefly, by homologous recombination, we disrupted exon 1, which contains the translation initiation

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K A Berghorn Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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P A Clark Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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B Encarnacion Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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C J DeRegis Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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J K Folger Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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M I Morasso Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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M J Soares Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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M W Wolfe Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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M S Roberson Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA
Developmental Skin Biology Unit, NIAMS, Bethesda, Maryland, USA
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA

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-gene cluster and the Dlx3 promoter by C/EBPβ in cells of trophoblast origin requires further study. An important gene target of Dlx3 is the human glycoprotein hormone α subunit gene promoter expressed in trophoblast cells ( Roberson et al. 2001 ). The α

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Mary C Gieske Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Gi Youn Na Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Yongbum Koo Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Misung Jo Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Thomas E Curry Jr Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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Chemyong Ko Department of Clinical Sciences and Biological Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, USA
School of Biotechnology and Biomedical Sciences, Inje University, Kimhae, South Korea

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, Deng C, Sahu A, Lambris JD & Song WC 1999 Role of decay-accelerating factor in regulating complement activation on the erythrocyte surface as revealed by gene targeting. PNAS 96 628 –633. Ushigoe K , Irahara M

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Sofianos Andrikopoulos University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Christine M Massa University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Kathryn Aston-Mourney University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Alexandra Funkat University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Barbara C Fam University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Rebecca L Hull University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Steven E Kahn University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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Joseph Proietto University of Melbourne, Department of Medicine (AH/NH), Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, VA Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA

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increase hybrid vigor as well as the increasing use of the Cre/LoxP system (which requires a tissue-specific Cre expressing and a LoxP-containing gene targeted mouse), the result0ant genetically modified mouse is frequently a conglomeration of two or more

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Bernard Freudenthal Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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John Logan Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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Sanger Institute Mouse Pipelines Mouse Pipelines, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

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Peter I Croucher Garvan Institute of Medical Research, Sydney, New South Wales, Australia

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Graham R Williams Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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J H Duncan Bassett Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK

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protein-coding genes in C57BL/6 mouse ES cells by 2021 ( Collins et al . 2007 ). The IKMC-targeted mutagenesis techniques involve use of bacterial artificial chromosome (BAC)-based ‘Knockout-First’ conditional ready gene targeting vectors that use

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