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Riccardo Dore Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Luka Levata Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Hendrik Lehnert Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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Carla Schulz Department of Internal Medicine I, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck, Lübeck, Germany

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, nesfatin-1 was demonstrated to be co-localized with oxytocin within the SON and PVN ( Foo et al. 2008 , Kohno et al. 2008 ). The excitability of oxytocinergic neurons of the PVN was influenced by nesfatin-1 in vitro ( Price et al. 2008 a

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Georgina G J Hazell
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Song T Yao
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James A Roper
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Eric R Prossnitz LINE, University of New Mexico, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK

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Anne-Marie O'Carroll
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Stephen J Lolait
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. 2009 , Otto et al . 2008 ). In addition, GPR30 is expressed in oxytocin (OXT) neurones of the rat hypothalamic paraventricular nucleus (PVH) and supraoptic nucleus (SON), implicating a role for GPR30 in the fast, non-genomic actions of E 2 on OXT

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Makoto Kawasaki Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Tatsushi Onaka Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Masamitsu Nakazato Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Jun Saito Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Takashi Mera Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Hirofumi Hashimoto Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Hiroaki Fujihara Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Nobukazu Okimoto Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Hideo Ohnishi Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Toshitaka Nakamura Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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Yoichi Ueta Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

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in the supraoptic nucleus (SON), which contains magnocellular neurosecretory cells (MNCs) that produce arginine vasopressin (AVP) and oxytocin (OXT) in rats ( Lee et al. 1999 , Dun et al. 2003 , Levine et al. 2005 ). However, there are few

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M Jankowski Laboratory of Cardiovascular Biochemistry, Centre de recherche, Centre hospitalier de l’Université de Montreal (CHUM) Montreal, Quebec, Canada

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D Wang Laboratory of Cardiovascular Biochemistry, Centre de recherche, Centre hospitalier de l’Université de Montreal (CHUM) Montreal, Quebec, Canada

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S Mukaddam-Daher Laboratory of Cardiovascular Biochemistry, Centre de recherche, Centre hospitalier de l’Université de Montreal (CHUM) Montreal, Quebec, Canada

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J Gutkowska Laboratory of Cardiovascular Biochemistry, Centre de recherche, Centre hospitalier de l’Université de Montreal (CHUM) Montreal, Quebec, Canada

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investigate the expression of natriuretic peptides, NOS and cGMP in the rat LV throughout gestation and early postpartum. Because of their contribution to ANP and NO release in the cardiovascular system, estrogen and oxytocin receptors (OTR) were also

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Anna Z Szóstek Department of Reproductive Immunology and Pathology, The Interdisciplinary Centre of Research in Animal Health, Institute of Animal Reproduction and Food Research, Olsztyn, Poland

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António M Galvão Department of Reproductive Immunology and Pathology, The Interdisciplinary Centre of Research in Animal Health, Institute of Animal Reproduction and Food Research, Olsztyn, Poland
Department of Reproductive Immunology and Pathology, The Interdisciplinary Centre of Research in Animal Health, Institute of Animal Reproduction and Food Research, Olsztyn, Poland

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Graça M Ferreira-Dias Department of Reproductive Immunology and Pathology, The Interdisciplinary Centre of Research in Animal Health, Institute of Animal Reproduction and Food Research, Olsztyn, Poland

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Dariusz J Skarzynski Department of Reproductive Immunology and Pathology, The Interdisciplinary Centre of Research in Animal Health, Institute of Animal Reproduction and Food Research, Olsztyn, Poland

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). Oxytocin (OT; 10 −7 M) was used as a positive control as described recently ( Szóstek et al . 2012 a , b ). In the above-mentioned study, we showed that epithelial as well as stromal cells are capable of producing PG in response to OT ( Szóstek et al

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Z Cheng Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK

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M Elmes Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK

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S E Kirkup Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK

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E C Chin Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK

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D R E Abayasekara Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK

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D C Wathes Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK

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expression at this time ( Zhang et al . 1996 , Gibb et al . 2000 , McClaren et al. 2000 ). In the uterus and placenta PG production and release can be modulated by a variety of physiological and pharmacological stimuli, for example oxytocin (OT; Lee

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G Almeida-Pereira Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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T Vilhena-Franco Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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R Coletti Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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S Q Cognuck Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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H V P Silva Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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L L K Elias Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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J Antunes-Rodrigues Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil

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octapeptide hormone angiotensin II (ANGII) also induces vasopressin (AVP) and oxytocin (OT) secretion when injected into the brain ( Antunes-Rodrigues et al . 2004 , Almeida-Pereira et al . 2016 ). OT and AVP are synthesized by magnocellular neurosecretory

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Jing Lu Department of OB/GYN, Department of OB/GYN, NorthShore University HealthSystem, 2650 Ridge Avenue, Evanston, Illinois 60201, USA
Department of OB/GYN, Department of OB/GYN, NorthShore University HealthSystem, 2650 Ridge Avenue, Evanston, Illinois 60201, USA

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Joshua Reese Department of OB/GYN, Department of OB/GYN, NorthShore University HealthSystem, 2650 Ridge Avenue, Evanston, Illinois 60201, USA

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Ying Zhou Department of OB/GYN, Department of OB/GYN, NorthShore University HealthSystem, 2650 Ridge Avenue, Evanston, Illinois 60201, USA

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Emmet Hirsch Department of OB/GYN, Department of OB/GYN, NorthShore University HealthSystem, 2650 Ridge Avenue, Evanston, Illinois 60201, USA
Department of OB/GYN, Department of OB/GYN, NorthShore University HealthSystem, 2650 Ridge Avenue, Evanston, Illinois 60201, USA

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1B (IL1B ( Il1B ), Mm00434228_m1), cyclooxygenase 2 (COX2 ( Ptgs2 ), Mm00478374_m1), iNOS ( Nos2 , Mm00440502_m1), oxytocin receptor ( Oxtr , Mm01182684_m1), Creb3 (Mm00457268_m1), and steroid receptor coactivator 2 (SRC2 ( Ncoa2 ), Mm00500749_m1

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Hwa-Yong Lee Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Tomas J Acosta Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Michiyo Tanikawa Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Ryosuke Sakumoto Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Junichi Komiyama Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Yukari Tasaki Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Mariusz Piskula Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Dariusz J Skarzynski Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Masafumi Tetsuka Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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Kiyoshi Okuda Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
Departments of Food Technology and
Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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of endometrial cells have the capacity to produce PGs, they have specific morphological and physiological properties ( Asselin et al. 1997 , Fortier et al. 1988 , Miyamoto et al. 2000 ). We found that oxytocin (OT) stimulates PG production

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Anna Fodor
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Ottó Pintér
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Ágnes Domokos
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Kristina Langnaese Institute of Experimental Medicine, Institut für Biochemie and Zellbiologie, Centre for Behavioral Brain Sciences, Hungarian Academy of Sciences, Szigony 43, 1083 Budapest, Hungary

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István Barna
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Mario Engelmann Institute of Experimental Medicine, Institut für Biochemie and Zellbiologie, Centre for Behavioral Brain Sciences, Hungarian Academy of Sciences, Szigony 43, 1083 Budapest, Hungary
Institute of Experimental Medicine, Institut für Biochemie and Zellbiologie, Centre for Behavioral Brain Sciences, Hungarian Academy of Sciences, Szigony 43, 1083 Budapest, Hungary

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Dóra Zelena
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to defined stressors, lactating rats exhibit – compared with virgin females – reduced ACTH, corticosterone, prolactin, catecholamine, and oxytocin (OT) responses. In the PVN of lactating rats, Crh mRNA levels fail to increase in response to a

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