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C Giuliani Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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M Saji Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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I Bucci Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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G Fiore Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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M Liberatore Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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D S Singer Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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F Monaco Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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L D Kohn Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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G Napolitano Unit of Endocrinology, Department of Medicine and Sciences of Aging, University ‘G D’Annunzio’ and Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Campus Universitario, via Colle dell’Ara, 66013 Chieti, Italy
Arthur G Janes Cancer Center and Richard J Solove Research Institute, Ohio State University, Columbus, Ohio 43210, USA
Unit of Immuno-oncology Aging Research Center, Ce.S.I., ‘Gabriele D’Annunzio’ University Foundation, Chieti-Pescara, Italy
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA

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regulation and identified the transcription factors and the cis -elements on the 5′-flanking regions of the class I gene involved ( Saji et al. 1992 a , 1992 b , 1997 , Giuliani et al. 1995 , Taniguchi et al. 1998 , Giuliani et al. 2000

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R L Moore Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of

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Y Dai Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of
Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of

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D V Faller Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of
Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of
Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of
Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of
Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of
Cancer Center, Medicine, Biochemistry, Pediatrics, Microbiology, Pathology and Laboratory Medicine, Departments of

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transcription factors ( Tsai & Omalley 1994 , Mangelsdorf et al . 1995 , Aoyagi & Archer 2008 ). Steroid hormone receptors play essential roles in numerous biological processes, such as homeostasis, metabolism, cell growth, and development ( Tsai & Omalley

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Ralf Baumeister Bio 3, Bioinformatics and Molecular Genetics, University of Freiburg, Germany
ZBSA – Freiburg Center for Systems Biology, University of Freiburg, Germany
Renal Division, University Hospital Freiburg, Germany

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Elke Schaffitzel Bio 3, Bioinformatics and Molecular Genetics, University of Freiburg, Germany
ZBSA – Freiburg Center for Systems Biology, University of Freiburg, Germany
Renal Division, University Hospital Freiburg, Germany

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Maren Hertweck Bio 3, Bioinformatics and Molecular Genetics, University of Freiburg, Germany
ZBSA – Freiburg Center for Systems Biology, University of Freiburg, Germany
Renal Division, University Hospital Freiburg, Germany

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negatively regulate the FOXO transcription factor DAF-16. Inhibition of the daf-2 receptor gene expression/activity or other genes in the insulin/IGF pathway can have three effects. (A) Early developmental downregulation, which affects many biological

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Yunshan Hu Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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Hong Bin Liu Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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Richard W Simpson Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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Anthony E Dear Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181
Australian Centre for Blood Diseases, Eastern Clinical Research Unit, Department of Diabetes and Endocrinology, Biotechnology Division, Monash University, 6th Floor Burnett Tower, 89 Commercial Road, Prahran, Melbourne, Victoria, Australia 3181

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promoter The human and mouse Nur77 promoters share highly conserved binding sites for NF-κB and AP-1 transcription factors ( Pei et al . 2005 ). To define the molecular mechanism of TZD-mediated inhibition of NUR77 expression, we performed EMSAs using

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Botao Du
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Masahide Ohmichi
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Kazuhiro Takahashi
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Jun Kawagoe
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Chika Ohshima
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Hideki Igarashi
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Akiko Mori-Abe
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Maki Saitoh
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Tsuyoshi Ohta
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Akira Ohishi
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Masakazu Doshida
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Naohiro Tezuka
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Toshifumi Takahashi
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Hirohisa Kurachi
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, Meyerson et al. 1997 , Nakamura et al. 1997 ). It was recently reported that TERT protects neurons against Aβ- ( Zhu et al. 2000 ) or tropic factor withdrawal- and glutamate ( Fu et al. 2000 )-induced apoptosis. There is abundant evidence

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David J Mellis Musculoskeletal Research Programme, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB252ZD, UK

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Cecile Itzstein Musculoskeletal Research Programme, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB252ZD, UK

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Miep H Helfrich Musculoskeletal Research Programme, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB252ZD, UK

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Julie C Crockett Musculoskeletal Research Programme, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB252ZD, UK

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signalling pathways leading to the activation of transcription factors, with the NFκB pathway being of particular importance. The control of osteoclast differentiation and function by signalling pathways is highlighted by conditions in which key components of

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R Wang
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J Li
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N Yashpal
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N Gao
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immunofluorescent staining was performed. There were two sets of co-expression pattern studies. First co-expression of nestin with transcription factors was examined. Co-staining of nestin with PDX-1 was observed at 15±4% in the monolayers after 2 months of culture

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Toshiaki Ishizuka Department of Pharmacology, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

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Takashi Hinata Department of Pharmacology, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

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Yasuhiro Watanabe Department of Pharmacology, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

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mouse MSCs to increase the expression and secretion of VEGF ( Kinnaird et al . 2004 b , Wang et al . 2007 ). Hypoxia-inducible gene transcription is regulated by hypoxia-inducible factor-1 (HIF-1), which is essential for adaptive cellular responses to

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L Bouraoui Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain

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J Gutiérrez Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain

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I Navarro Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain

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(PPARγ) have been identified as transcriptional factors ( MacDougald & Lane 1995 ) and are necessary for the transition of pre-adipocytes into adipocytes in vitro . In mammals, insulin growth factor-I (IGF-I) affects adipocyte proliferation and

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Inga K Johnsen Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Marc Slawik Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Igor Shapiro Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Michaela F Hartmann Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Stefan A Wudy Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Brendan D Looyenga Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Gary D Hammer Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Martin Reincke Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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Felix Beuschlein Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert-Ludwigs-Universität, D-79106 Freiburg, Germany
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK
Steroid Research Unit, Center of Child and Adolescent Medicine, Justus-Liebig-University Giessen, Germany
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Medizinische Klinik - Innenstadt, Ludwig-Maximilians-University, Munich, Germany

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factors such as GATA-4 ( P = 0.0005), Wilms tumor gene-1 (WT-1; P < 0.0001), and steriodogenic factor-1 (SF-1; P = 0.0029) were significantly up-regulated after GDX, while other transcription factors such as FOG-1 ( P < 0.0001) and GATA-6 ( P = 0

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