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Russell T Turner Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences
Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences

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Kenneth A Philbrick Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences

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Carmen P Wong Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences

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Dawn A Olson Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences

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Adam J Branscum Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences

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Urszula T Iwaniec Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences
Skeletal Biology Laboratory, Center for Healthy Aging Research, Biostatistics, School of Biological and Population Health Sciences

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dual-energy X-ray absorptiometry (DXA), microcomputed tomography (μCT), and histomorphometry. Tibiae were removed, cleaned of soft tissue, frozen in liquid nitrogen, and stored at −80 °C for mRNA isolation and gene expression analysis. Serum chemistry

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-14-0224
Volume/Issue:
Volume 223: Issue 1
Free access
Thomas Funck-Brentano Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Karin H Nilsson Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Robert Brommage Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Petra Henning Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Ulf H Lerner Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Antti Koskela Unit of Cancer Research and Translational Medicine, MRC Oulu and Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland

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Juha Tuukkanen Unit of Cancer Research and Translational Medicine, MRC Oulu and Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland

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Martine Cohen-Solal BIOSCAR UMRS 1132, Université Paris Diderot, Sorbonne Paris Cité, INSERM, Paris, France

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Sofia Movérare-Skrtic Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Claes Ohlsson Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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(Instron Corp., Norwood, MA, USA). Based on the recorded load deformation curves, the biomechanical parameters were calculated from raw files produced by Bluehill 2 software, version 2.6 (Instron) with custom-made Excel macros. Bone histomorphometry

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-18-0153
Volume/Issue:
Volume 238: Issue 1
Open access
David E Maridas Maine Medical Center Research Institute, Scarborough, Maine, USA

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Victoria E DeMambro Maine Medical Center Research Institute, Scarborough, Maine, USA

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Phuong T Le Maine Medical Center Research Institute, Scarborough, Maine, USA

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Kenichi Nagano Harvard School of Dental Medicine, Boston, Massachusetts, USA

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Roland Baron Harvard School of Dental Medicine, Boston, Massachusetts, USA

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Subburaman Mohan VA Loma Linda Healthcare System, Loma Linda, California, USA

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Clifford J Rosen Maine Medical Center Research Institute, Scarborough, Maine, USA

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. Histomorphometry Calcein and demeclocycline were injected intraperitoneally at 20mg/kg of body weight at respectively 9 days and 2 days prior to killing the mice at 16 weeks of age. Tibiae were fixed for 48 h in 10% buffered formalin and stored in 70% ethanol

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-16-0673
Volume/Issue:
Volume 233: Issue 1
Open access
Vikte Lionikaite Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Karin L Gustafsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Anna Westerlund Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Sara H Windahl Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Antti Koskela Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, Oulu, Finland

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Juha Tuukkanen Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, Oulu, Finland

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Helena Johansson Institute for Health and Aging, Catholic University of Australia, Melbourne, Australia

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Claes Ohlsson Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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H Herschel Conaway Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Petra Henning Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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Ulf H Lerner Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

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reconstruction following established guidelines ( Bouxsein et al. 2010 ). Histomorphometry and measurement of mechanical strength For the measure of dynamic bone parameters, mice were injected (i.p.) with 100 µL of calcein (50 mg/kg) 8 and 1 day before

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-18-0316
Volume/Issue:
Volume 239: Issue 3
Open access
Claire L Wood Division of Functional Genetics and Development, Roslin Institute, University of Edinburgh, Edinburgh, UK
Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK

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Rob van ‘t Hof Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK

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Scott Dillon Division of Functional Genetics and Development, Roslin Institute, University of Edinburgh, Edinburgh, UK

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Volker Straub John Walton Muscular Dystrophy Research Centre, Newcastle University and Newcastle Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK

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Sze C Wong Developmental Endocrinology Research Group, School of Medicine, University of Glasgow, Glasgow, UK

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S Faisal Ahmed Developmental Endocrinology Research Group, School of Medicine, University of Glasgow, Glasgow, UK

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Colin Farquharson Division of Functional Genetics and Development, Roslin Institute, University of Edinburgh, Edinburgh, UK

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, West Sussex, UK). A 100 N loading cell was used with the span fixed at 10 mm and the cross-head was lowered at 1 mm/min to determine the load to failure and maximum stiffness and deflection of tibiae ( Huesa et al. 2011 ). Bone histomorphometry

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-21-0388
Volume/Issue:
Volume 253: Issue 2
Open access
Koichiro Komatsu Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Akemi Shimada Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Tatsuya Shibata Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Satoshi Wada Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Hisashi Ideno Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan
Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Kazuhisa Nakashima Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Norio Amizuka Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Masaki Noda Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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Akira Nifuji Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan
Departments of Pharmacology, Orthodontics, Transcriptome Research Group, Department of Developmental Biology of Hard Tissue, Department of Molecular Pharmacology, School of Dental Medicine, Tsurumi University, 2‐1‐3 Tsurumi, Tsurumi‐ku, Yokohama 230-8501, Japan

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( n =4), and 1 mM DC ( n =4) for 5 min at room temperature, and put back into their sockets. At certain time points, tissues were fixed and subjected to bone histomorphometry and immunohistochemistry (see below). Bone histomorphometry Animals in the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-13-0040
Volume/Issue:
Volume 219: Issue 2
Free access
J Jeyabalan
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M Shah
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B Viollet Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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J P Roux Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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P Chavassieux Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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M Korbonits Department of Veterinary Basic Sciences, INSERM, CNRS, Université Paris Decartes, INSERM, Department of Endocrinology, Royal Veterinary College, Royal College Street, London NW1 0TU, UK

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C Chenu
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tibiae were harvested from these mice for micro-CT (studies 1, 2 and 3) and bone histomorphometric analyses (study 1) respectively. Femora were collected for western blot and RT-PCR analyses. Histomorphometry analysis of tibia Right tibia from sham

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-12-0184
Volume/Issue:
Volume 214: Issue 3
Open access
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Russell T Turner Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA
Center for Healthy Aging Research, Oregon State University, Corvallis, Oregon, USA

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Kenneth A Philbrick Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA

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Amida F Kuah Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA

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Adam J Branscum Biostatistics Program, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA

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Urszula T Iwaniec Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA
Center for Healthy Aging Research, Oregon State University, Corvallis, Oregon, USA

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epiphysis and in lumbar vertebra included cancellous bone volume fraction (bone volume/tissue volume, %), trabecular thickness (µm), trabecular number (/mm), and trabecular separation (µm). Histomorphometry Distal femora were dehydrated in a graded

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-17-0103
Volume/Issue:
Volume 233: Issue 3
Free access
Zuzana Saidak UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France
UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France

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Carole Le Henaff UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France
UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France

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Sofia Azzi UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France
UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France

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Caroline Marty UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France
UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France

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Pierre J Marie UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France
UMR‐1132 Inserm, Université Paris Diderot, Hôpital Lariboisière, 2 Rue Ambroise Paré, 75475 Paris Cedex 10, France

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, by ELISA (R&D Systems, Lille, France). Bone microarchitecture and histomorphometry The animals were killed and, lumbar vertebrae and right femurs (distal metaphysis) were obtained for analysis of micro- and macro-structures. The bones were scanned

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-14-0249
Volume/Issue:
Volume 223: Issue 1
Free access
M M Conradie
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H de Wet
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D D R Kotze
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J M Burrin
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F S Hough
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P A Hulley
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,25-Dihydroxyvitamin D3 modulates glucocorticoid-induced alteration in serum bone Gla protein and bone histomorphometry. Endocrinology 120 531 –536. Karsdal MA , Larsen L, Engsig MT, Lou H, Ferreras M, Lochter A, Delaisse JM & Foged NT

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-07-0217
Volume/Issue:
Volume 195: Issue 2
Free access