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Neurofisiología Molecular, Escuela de Dietética y Nutrición, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz (INPRFM), Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, C.P. 14370, México, Distrito Federal, México
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pro-corticotropin-releasing factor (proCRF), which regulate the hypothalamus–pituitary–thyroid (HPT) and hypothalamus–pituitary–adrenal (HPA) axes. This responsiveness was assessed after the known 48 h fasting-induced shift in leptin and corticosterone
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percentage of C values (100%; n = 4–5/group). One-way ANOVA followed by Tukey’s post hoc test showed differences between groups, * P < 0.05 vs C; # P < 0.05 vs DPF. Figure 3 proCRF mRNA expression in PVN. (A–C) Low-power 10
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tissues were snap frozen in liquid N 2 and stored at −80 °C until total RNA isolation and CRF extraction. The obtained RNA was assayed for levels of chicken CRF precursor (proCRF) ( Vandenborne et al. 2005 ), POMC ( Gerets et al. 2000 ) and CRF
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1157 – E1163 . ( doi:10.1152/ajpendo.90869.2008 ) Aréchiga-Ceballos F Alvarez-Salas E Matamoros-Trejo G Amaya MI García-Luna C de Gortari P 2014 Pro-TRH and pro-CRF expression in paraventricular nucleus of small litter-reared fasted