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M Victoria Recouvreux Instituto de Biología y Medicina Experimental, Department of Medicine, Department of Cell Biology, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Department of Medicine, Department of Cell Biology, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina

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M Andrea Camilletti Instituto de Biología y Medicina Experimental, Department of Medicine, Department of Cell Biology, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina

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Daniel B Rifkin Instituto de Biología y Medicina Experimental, Department of Medicine, Department of Cell Biology, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina

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Graciela Díaz-Torga Instituto de Biología y Medicina Experimental, Department of Medicine, Department of Cell Biology, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina

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, to restore gonadal function, to reduce tumor size, and to preserve or improve residual pituitary function. Prolactin secretion in the normal pituitary is tonically inhibited by hypothalamic dopamine through dopamine D2 receptors (Drd2) expressed on

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Hiroharu Mifune Institute of Animal Experimentation, Kurume University School of Medicine, Kurume, Japan

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Yuji Tajiri Division of Endocrinology and Metabolism, Kurume University School of Medicine, Kurume, Japan

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Yusuke Sakai Institute of Animal Experimentation, Kurume University School of Medicine, Kurume, Japan

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Yukie Kawahara Department of Pharmacology, Kurume University School of Medicine, Kurume, Japan

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Kento Hara Division of Endocrinology and Metabolism, Kurume University School of Medicine, Kurume, Japan

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Takahiro Sato Molecular Genetics, Life Science Institute, Kurume University, Kurume, Japan

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Yoshihiro Nishi Department of Physiology, Kurume University School of Medicine, Kurume, Japan

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Akinori Nishi Department of Pharmacology, Kurume University School of Medicine, Kurume, Japan

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Ryouichi Mitsuzono Department of Exercise Physiology, Institute of Health and Sports Science, Kurume University, Kurume, Japan

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Tatsuyuki Kakuma Bostatistics Center, Kurume University, Kurume, Japan

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Masayasu Kojima Molecular Genetics, Life Science Institute, Kurume University, Kurume, Japan

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cerebral cortex and nucleus accumbens (NAc) ( Phillips et al. 2007 ). Recent studies have shown that mesolimbic dopamine neurons in the ventral tegmental area (VTA) that project to the NAc represent a critical site for ghrelin to trigger food consumption

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Ebony T Gilbreath Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA
Department of Pathobiology, College of Veterinary Medicine, Tuskegee University, Tuskegee, Alabama, USA

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Lakshmikripa Jaganathan Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA

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Madhan Subramanian Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA

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Priya Balasubramanian Department of Pharmacology and Toxicology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA

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Katrina D Linning Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA

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Sheba M J MohanKumar Department of Pharmacology and Toxicology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA
Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA

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Puliyur S MohanKumar Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA
Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA

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reach middle age. During this time, serum estradiol levels are moderately elevated for a period of 6 months. This results in a gradual decline in hypothalamic dopamine levels with a concurrent increase in serum prolactin levels. This chronic

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Helen L Henderson Department of Anatomy, University of Bristol, Bristol BS2 8EJ, England, UK

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Julie Townsend Department of Anatomy, University of Bristol, Bristol BS2 8EJ, England, UK

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Domingo J Tortonese Department of Anatomy, University of Bristol, Bristol BS2 8EJ, England, UK

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, our laboratory demonstrated a seasonal modulation of the combined suppressive effects of PRL and dopamine on the gonadotrophin response to GnRH ( Gregory et al . 2004 ). An immortalised gonadotroph cell line, αT 3 -1, has been shown to express D 2

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M Arvigo
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F Gatto
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M Ruscica Department of Endocrinological and Medical Sciences and Center of Excellence for Biomedical Research, Department of Endocrinology, Department of Human Morphology and Biomedical Sciences ‘Citta' Studi’, Biomeasure Incorporated/IPSEN, Università degli Studi di Genova, 16132 Genova, Italy

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P Ameri
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E Dozio Department of Endocrinological and Medical Sciences and Center of Excellence for Biomedical Research, Department of Endocrinology, Department of Human Morphology and Biomedical Sciences ‘Citta' Studi’, Biomeasure Incorporated/IPSEN, Università degli Studi di Genova, 16132 Genova, Italy

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M Albertelli
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M D Culler Department of Endocrinological and Medical Sciences and Center of Excellence for Biomedical Research, Department of Endocrinology, Department of Human Morphology and Biomedical Sciences ‘Citta' Studi’, Biomeasure Incorporated/IPSEN, Università degli Studi di Genova, 16132 Genova, Italy

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M Motta Department of Endocrinological and Medical Sciences and Center of Excellence for Biomedical Research, Department of Endocrinology, Department of Human Morphology and Biomedical Sciences ‘Citta' Studi’, Biomeasure Incorporated/IPSEN, Università degli Studi di Genova, 16132 Genova, Italy

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F Minuto
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P Magni Department of Endocrinological and Medical Sciences and Center of Excellence for Biomedical Research, Department of Endocrinology, Department of Human Morphology and Biomedical Sciences ‘Citta' Studi’, Biomeasure Incorporated/IPSEN, Università degli Studi di Genova, 16132 Genova, Italy

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D Ferone
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heterodimerize with other G-protein-coupled receptors, such as the μ-opioid receptor ( Pfeiffer et al . 2002 ) and dopamine (DA) type 2 receptor (D 2 R; Rocheville et al . 2000 b , Baragli et al . 2007 ). The heterodimerization of these G

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Anthony Raffo Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Kolbe Hancock Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Teresa Polito Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Yuli Xie Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Gordon Andan Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Piotr Witkowski Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA
Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Mark Hardy Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Pasquale Barba Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Caterina Ferrara Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Antonella Maffei Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA
Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Matthew Freeby Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Robin Goland Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Rudolph L Leibel Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Ian R Sweet Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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Paul E Harris Department of Medicine, Barnard College, Department of Surgery of Columbia University Medical Center, Department of Surgery, Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, Naomi Berrie Diabetes Center, Department of Medicine, BB 20-06, Columbia University Medical Center, 650 West 168th Street, New York, New York 10032, USA

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2000 ). β-Cells of the endocrine pancreas also express dopamine receptors (D2) and respond to exogenous dopamine with inhibited glucose-stimulated insulin secretion ( Ahren & Lundquist 1985 , Niswender et al . 2005 , Rubi et al . 2005 , Shankar et

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David R Grattan Centre for Neuroendocrinology and Department of Anatomy, Maurice Wilkins Centre for Molecular Biodiscovery, University of Otago, PO Box 913, Dunedin 9054, New Zealand
Centre for Neuroendocrinology and Department of Anatomy, Maurice Wilkins Centre for Molecular Biodiscovery, University of Otago, PO Box 913, Dunedin 9054, New Zealand

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catecholamine neurotransmitter, dopamine, rather than the more typical peptide hypothalamic hormones involved in regulating all other pituitary systems. Prolactin is also the only anterior pituitary hormone that does not have an endocrine target tissue, and

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Chunxiao Qi Department of Neurobiology, Hebei Medical University, Shijiazhuang, People’s Republic of China
Department of Human Anatomy, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Xiaoming Ji Department of Neurobiology, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Guoliang Zhang Department of Human Anatomy, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Yunxiao Kang Department of Neurobiology, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Yuanxiang Huang Grade 2015 Eight-year Clinical Medicine Program, School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Rui Cui Department of Human Anatomy, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Shuangcheng Li Department of Human Anatomy, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Huixian Cui Department of Human Anatomy, Hebei Medical University, Shijiazhuang, People’s Republic of China
Neuroscience Research Center, Hebei Medical University, Shijiazhuang, People’s Republic of China

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Geming Shi Department of Neurobiology, Hebei Medical University, Shijiazhuang, People’s Republic of China
Department of Human Anatomy, Hebei Medical University, Shijiazhuang, People’s Republic of China
Neuroscience Research Center, Hebei Medical University, Shijiazhuang, People’s Republic of China

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neurotransmissions. An imbalance in dopamine (DA) neurotransmissions leads to either hyperactive or hypoactive neuropsychiatric disorders ( Mehler-Wex et al . 2006 , Howes & Kapur 2009 , Leckman et al . 2010 , Chen et al . 2013 ). Elevation of DA, as a driver

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Isabel García-Tornadú Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Marcelo Rubinstein Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Bruce D Gaylinn Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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David Hill Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Edith Arany Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Malcolm J Low Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Graciela Díaz-Torga Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Damasia Becu-Villalobos Instituto de Biología y Medicina Experimental and
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and University of Buenos Aires, V. Obligado 2490, 1428 Buenos Aires, Argentina
UVA Health System Charlottesville, Virginia, USA
Lawson Health Research Institute, London, Ontario, Canada
Center for the Study of Weight Regulation, Oregon Health & Science University, Portland, Oregon, USA

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Introduction The dopamine D2 receptor (D2R) is the predominant dopamine-receptor subtype in the anterior pituitary and mediates dopamine’s inhibitory actions on lactotropes ( Missale et al. 1998 , Ben-Jonathan & Hnasko 2001

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Erik J Giltay Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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King H Kho Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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Ben A Blansjaar Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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Marcel M Verbeek Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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P Ben H Geurtz Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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Johanna M Geleijnse Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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Louis J G Gooren Geestelijke Gezondheidszorg Delfland, Institute of Mental Health, PO Box 5016, 2600 GA Delft, The Netherlands
Radboud University Nijmegen Medical Centre, Department of Neurology, Laboratory of Pediatrics and Neurology, Nijmegen, The Netherlands
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
Department of Endocrinology, Andrology Unit, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands

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metabolizing these steroids ( Puy et al. 1995 , Poletti & Martini 1999 , Stoffel-Wagner 2003 , Altman 2004 ). Dopamine is synthesized from tyrosine by dopaminergic neurons, and is also the precursor to norepinephrine in noradrenergic nerves. Dopamine is

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