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Alison Mostyn UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Linda Attig UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Thibaut Larcher UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Samir Dou UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Pascale Chavatte-Palmer UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Monia Boukthir UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Arieh Gertler UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Jean Djiane UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Michael E Symonds UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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Latifa Abdennebi-Najar UP 2012.10.101 EGEAL, School of Veterinary Medicine and Science, INRA UMR 703, INRA, Unité de Recherche 04UR08/03, The Hebrew University of Jerusalem, Unité NOPA, Early Life Research Unit, Institut Polytechnique LaSalle, Beauvais, France

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due to an open reading frame on the BAT-specific uncoupling protein 1 ( UCP1 ) gene in the porcine species meaning that UCP1 is not expressed ( Berg et al . 2006 ). However, it has more recently been suggested that BAT is present in young piglets

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Zhen-Chuan Fan Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519, USA

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James L Sartin Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519, USA

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Ya-Xiong Tao Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519, USA

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cloning of porcine MC3R Sequence analysis performed with DNAman program (Lynnon Corp., Quebec, Canada) suggested that the putative coding region of the pMC3R gene consists of a single exon of 960 bp. Thus, the pMC3R coding region was amplified directly

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George Harb Department of Surgery, Surgical-Medical Research Institute, 1074 Dentistry Pharmacy Center, University of Alberta, Alberta, Edmonton, Canada T6G 2N8

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Gregory S Korbutt Department of Surgery, Surgical-Medical Research Institute, 1074 Dentistry Pharmacy Center, University of Alberta, Alberta, Edmonton, Canada T6G 2N8

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-responsive ( Kuhl et al. 1980 ). Islets from neonatal animals like pigs provide an interesting model to study the effects of high glucose exposure on β-cell function and survival as well as differentiation from precursors. Neonatal porcine islets contain

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M S Pampusch Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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G Xi Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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E Kamanga-Sollo Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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K J Loseth Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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M R Hathaway Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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W R Dayton Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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M E White Animal Growth and Development Laboratory, Department of Animal Science, University of Minnesota, St Paul, Minnesota 55108, USA

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of IGFBP-5 as well as putative IGFBP-5 receptors may be responsible for the IGF-independent actions of IGFBP-5 on cells. IGFBP-5 is produced by myogenic cell lines, porcine embryonic myogenic cells (PEMCs), and porcine muscle satellite cells

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Xiufen Chen State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Bo Zhou State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Jun Yan State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Baoshan Xu State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Ping Tai State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Junxia Li State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Shiming Peng State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Meijia Zhang State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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Guoliang Xia State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

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cumulus-enclosed oocyte maturation. EGF, by the same receptor EGFR as that of EGF-like factors ( Harris et al . 2003 ), has also been shown to mimic FSH-induced porcine oocyte meiotic maturation and development competence of oocytes ( Singh et al . 1993

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Eleonore Fröhlich
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Anja Witke
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Barbara Czarnocka
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Richard Wahl
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). Using normal porcine thyrocytes as a culture model, we aimed to detect conditions under which no dedifferentiating effects on normal thyrocytes were observed and to detect effects that may be initiated after short periods of incubation. We determined

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Chao Li Department of Surgery, The Second Affiliated Hospital of Zhejiang University, Hanghzou, Zhejiang, China

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Bin Yang Department of Surgery, The Second Affiliated Hospital of Zhejiang University, Hanghzou, Zhejiang, China

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Zhihao Xu Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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Eric Boivin Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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Mazzen Black Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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Wenlong Huang Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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Baoyou Xu Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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Ping Wu Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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Bo Zhang Department of Surgery, The Second Affiliated Hospital of Zhejiang University, Hanghzou, Zhejiang, China

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Xian Li Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China

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Kunsong Chen Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China

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Yulian Wu Department of Surgery, The Second Affiliated Hospital of Zhejiang University, Hanghzou, Zhejiang, China

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Gina R Rayat Department of Surgery, Ray Rajotte Surgical-Medical Research Institute, Alberta Diabetes Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada

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of islet transplantation ( Mohseni Salehi Monfared et al . 2009 ). For example, vitamins D3 and E, which are known to have antioxidant properties, were used to improve the morphology and function of neonatal porcine islets (NPI) ( Luca et al . 2000

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A V Sirotkin
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P Sanislo
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H-J Schaeffer
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I Florkovičová
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J Kotwica
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J Bulla
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L Hetényi
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associated with proliferation (PCNA), apoptosis (Bax), TK (phosphotyrosine), Cdc2/p34, PKA and PKA-activated transcription factor (CREB-1) in cultured porcine ovarian cells. We also investigated how TPO influences the secretion of progesterone

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María E Trujillo-Ortega Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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Daniel Mota-Rojas Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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Rafael Hernández-González Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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Elvia Yadira Velázquez-Armenta Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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Alejandro A Nava-Ocampo Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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Ramiro Ramírez-Necoechea Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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Marcelino Becerril-Herrera Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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María Alonso-Spilsbury Department of Animal Production: Swine, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México
Department of Animal Production and Agriculture, Research Area: Ecodesarrollo de la Producción Animal, School of Veterinary Medicine, Universidad Autónoma Metropolitana-Xochimilco, México
Departamento de Investigación Experimental y Bioterio, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada
EIAH-Benemérita Universidad Autónoma de Puebla, San Juan Acateno, Teziutlan, Puebla, México

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region of either 1 ml saline ( n =10 sows) or 6 mg/day recombinant porcine ST (rpST) (Reporcin, Alpharma Animal Health Pty. Ltd, Victoria, Australia) in 1 ml saline ( n =10 sows) from days 80 to 114 of gestation. The dose of rpST had previously been

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MS Pampusch
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E Kamanga-Sollo
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ME White
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MR Hathaway
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WR Dayton
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IGF-binding protein (IGFBP)-3 is produced by cultured porcine embryonic myogenic cell (PEMC) cultures and is secreted into the medium. Levels of secreted IGFBP-3 and IGFBP-3 mRNA are significantly reduced during differentiation and increase after differentiation is complete, suggesting that IGFBP-3 may play some role in myogenesis and/or in changes in myogenic cell proliferation that accompany differentiation. IGFBP-3 reportedly may either suppress or stimulate proliferation of cultured cells depending on cell type. Additionally, IGFBP-3 has been shown to affect proliferation via both IGF-dependent and IGF-independent mechanisms in some cell types but not all. Currently, the effect, if any, of IGFBP-3 on myogenic cell proliferation is not known. Consequently, the goal of this study was to assess the IGF-I-dependent and IGF-I-independent actions of recombinant porcine IGFBP-3 on proliferation of cultured porcine myogenic cells. To facilitate these investigations, we have expressed porcine IGFBP-3 in the baculovirus system, purified and characterized the expressed recombinant porcine IGFBP-3 (rpIGFBP-3), and produced and characterized an anti-porcine IGFBP-3 antibody that neutralizes the biological activity of porcine IGFBP-3. rpIGFBP-3 suppressed IGF-I-stimulated proliferation of PEMCs in a concentration-dependent manner with equimolar concentrations of IGF-I and rpIGFBP-3, resulting in complete suppression of IGF-I-stimulated proliferation. rpIGFBP-3 also suppressed Long-R3-IGF-I-stimulated proliferation of PEMC, indicating that rpIGFBP-3 possesses IGF-independent activity in this cell system. These data have established that IGFBP-3 has the potential to affect proliferation of PEMCs during critical periods of muscle development that may impact ultimate muscle mass achievable postnatally.

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