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T Taguchi, T Takao, Y Iwasaki, M Nishiyama, K Asaba, and K Hashimoto

-FITC (PharMingen, San Diego, CA, USA) and propidium iodide (PI; 100 μg/ml), and were subsequently analyzed by flow cytometry with a fluorescence-activated cell sorter (FACStar; Becton Dickinson, San Jose, CA, USA). 3-[4,5-dimethylthiazol-2

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Y Wang, L P Wu, J Fu, H J Lv, X Y Guan, L Xu, P Chen, C Q Gao, P Hou, M J Ji, and B Y Shi

suspensions were added to anti-mCD4-FITC and anti-mCD25-PE antibodies (L200 and M-A251, respectively; BD Biosciences, San Jose, CA, USA) and then incubated at 4 °C for 10 min. We sorted CD4+ CD25+ Treg cells with flow cytometry (FACSAria, BD) and collected CD4

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Vicki E Smith, Jayne A Franklyn, and Christopher J McCabe

tyrosine-based sorting signal at the C-terminus. M2 (Δ29–93) and M3 (Δ94–149) appeared to be predominantly expressed in the endoplasmic reticulum. Markedly, none of the mutants colocalised with CD63, strongly suggesting that they were not expressed within

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Jane A Mitchell, Mark J Paul-Clark, Graham W Clarke, Shaun K McMaster, and Neil Cartwright

2006 Membrane sorting of Toll-like receptor (TLR)-2/6 and TLR2/1 heterodimers at the cell surface determines heterotypic associations with CD36 and intracellular targeting. Journal of Biological Chemistry 281 31002 –31011

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J Miao, K-W Chan, G G Chen, S-Y Chun, N-S Xia, J Y H Chan, and N S Panesar

and mammary gland. Journal of Molecular Endocrinology 26 11 –19. Pfanner N 2000 Protein sorting: recognizing mitochondrial presequences. Current Biology 10 412 –415. Poon HK , Chan JY

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Elizabeth S Barrie, Mels Lodder, Paul H Weinreb, Jill Buss, Amer Rajab, Christopher Adin, Qing-Sheng Mi, and Gregg A Hadley

, exon 10 of the integrin alpha E ( Itgae ) locus was replaced with a neomycin resistance gene. The deficiency was detected via PCR, fluorescence-activated cell sorting (FACS) analysis, and immunoprecipitation as an insertion of approximately 200 bp

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Wenbin Shang, Ying Yang, Libin Zhou, Boren Jiang, Hua Jin, and Mingdao Chen

membrane . Diabetes 53 41 – 52 . Piper RC Hess LJ James DE 1991 Differential sorting of two glucose transporters expressed in insulin-sensitive cells . American Journal of Physiology 260 C570 – C580 . Reay JL Kennedy DO Scholey AB

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Eun-Kyung Choi, Woon-Ki Kim, Ok-Joo Sul, Yun-Kyung Park, Eun-Sook Kim, Jae-Hee Suh, Rina Yu, and Hye-Seon Choi

determined by Amplex Red hydrogen peroxide/peroxidase assay kit (Invitrogen). Stromal vascular cell isolation Visceral fat pads were weighed, rinsed three times in PBS, and minced with fluorescence-activated cell sorter (FACS) buffer (PBS with 1% BSA). Tissue

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V Rider, T Potapova, G Dai, and M J Soares

Ploidy and progesterone-receptor distribution in flow sorted deciduomal nuclei. Molecular and Cellular Endocrinology 25 99 –104. Okuda M , Horn HF, Tarapore P, Tokuyama Y, Smulian AG, Chan PK, Knudsen ES, Hofmann IA

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François van Herp, Nick H M van Bakel, Anton J M Coenen, Kjell Sergeant, Bart Devreese, and Gerard J M Martens

assembled to achieve their correct tertiary structures, whereas the Golgi controls a number of post-translational modifications and the sorting event. In the secretory granules of neuronal and endocrine cells, prohormones are proteolytically cleaved to