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Alessandra Fierabracci

dye and then analysed by fluorescence-activating cell sorter (FACS) analysis ( Goodell et al . 1996 ). It was subsequently proven that the expression of ATP-binding cassette-dependent transporter ABCG2 ( Thomas et al . 2008 ) was responsible for this

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Kotaro Horiguchi, Tom Kouki, Ken Fujiwara, Takehiro Tsukada, Floren Ly, Motoshi Kikuchi, and Takashi Yashiro

0.5 μg/ml of streptomycin (Invitrogen). Other dispersed cells were separated into GFP-positive and GFP-negative cells by a cell sorter (MoFlo XDP: Beckman Coulter, Inc., Fullerton, CA, USA). GFP-positive cells were plated onto eight-well glass

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Kotaro Horiguchi, Motoshi Kikuchi, Kenji Kusumoto, Ken Fujiwara, Tom Kouki, Kotaro Kawanishi, and Takashi Yashiro

prepared with Trizol reagent (Invitrogen) from anterior pituitary glands, anterior pituitary cell primary culture, and the GFP-positive cell fraction of S100b–GFP male rats. They were then sorted by a MoFlo XDP (Beckman Coulter, Inc., Fullerton, CA, USA

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L B Hays, B Wicksteed, Y Wang, J F McCuaig, L H Philipson, J M Edwardson, and C J Rhodes

pituitary cells ( Hodel & Edwardson 2000 ). However, at the trans -Golgi network, newly synthesized syncollin could be sorted to either the constitutive or regulated secretory pathways ( Arvan & Castle 1998 ). Consequently, we examined whether syncollin

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Masaki Nagaya, Hitoshi Katsuta, Hideaki Kaneto, Susan Bonner-Weir, and Gordon C Weir

applied to each well. PCR was performed with the following parameters: 36 cycles of 30 s at 94 °C, 90 s at 60 °C, 90 s at 72 °C. Cell sorting All samples were analyzed on an ARIA cell sorter with the Summit software (BD). The cells were dispersed to mostly

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Jonathan H Gooi, Ling Yeong Chia, Nicole C Walsh, Morten A Karsdal, Julian M W Quinn, T John Martin, and Natalie A Sims

sorted for GFP on a FACS Aria (BD Biosciences) as previously described ( Gooi et al . 2010 ). 100% GFP+ cells were used for cDNA preparations; this population made up an average of 1.63% of the sorted calvarial cells (range was <0.1–2.7%) and 0.45% of

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Michelle C Melo, Eva Andersson, Per Gunnar Fjelldal, Jan Bogerd, Luiz R França, Geir Lasse Taranger, and Rüdiger W Schulz

stage. The GSI values of the eight males sampled on January 5 showed a bimodal distribution (<0.04 vs >0.05; n =4 in both modes). After sorting the data on pituitary gene expression, androgen plasma levels, and proliferation activity in an immature and

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William F Powell Jr, Kevin J Barry, Irena Tulum, Tatsuya Kobayashi, Stephen E Harris, F Richard Bringhurst, and Paola Divieti Pajevic

weight, and the data were expressed as picomole of cAMP produced per mg of dry bone. Each experiment was repeated at least three times. Fluorescence-activated cell sorting analysis In some experiments, homozygous Dmp1 -GFP transgenic mice (provided

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C Zermeño, J Guzmán-Morales, Y Macotela, G Nava, F López-Barrera, J B Kouri, C Lavalle, G Martínez de la Escalera, and C Clapp

stained with propidium iodide (50 μg/ml) for 15 min at 4 ° C in the dark. Propidium iodide fluorescence of nuclei was measured by flow cytometry on a fluorescence-activated cell sorter (Becton Dickinson, Franklin Lakes, NJ, USA) with a 560 nm dichromatic

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Michael Wallis

mammalian prolactins, and emphasized the remarkable variability within Afrotheria. Adaptive evolution of prolactin in Afrotheria Episodes of rapid change of the sort identified in lineages leading to elephant and hyrax prolactins can result from two