Filter

Refine by date

—

Refine by access

  • Print
  • Save

Search Results

You are looking at 1 - 1 of 1 items for

  • Author: Yushi Nakayama x
  • Refine by access: All content x
Clear All Modify Search
Koji Eguchi Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Koji Eguchi in
Google Scholar
PubMed Close
,
Yuichiro Izumi Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Yuichiro Izumi in
Google Scholar
PubMed Close
,
Yukiko Yasuoka Department of Physiology, Kitasato University School of Medicine, Minami-ku, Sagamihara, Kanagawa, Japan

Search for other papers by Yukiko Yasuoka in
Google Scholar
PubMed Close
,
Terumasa Nakagawa Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Terumasa Nakagawa in
Google Scholar
PubMed Close
,
Makoto Ono Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Makoto Ono in
Google Scholar
PubMed Close
,
Kosuke Maruyama Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Kosuke Maruyama in
Google Scholar
PubMed Close
,
Naomi Matsuo Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Naomi Matsuo in
Google Scholar
PubMed Close
,
Akiko Hiramatsu Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Akiko Hiramatsu in
Google Scholar
PubMed Close
,
Hideki Inoue Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Hideki Inoue in
Google Scholar
PubMed Close
,
Yushi Nakayama Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Yushi Nakayama in
Google Scholar
PubMed Close
,
Hiroshi Nonoguchi Division of Internal Medicine, Kitasato University Medical Center, Kitamoto, Saitama, Japan

Search for other papers by Hiroshi Nonoguchi in
Google Scholar
PubMed Close
,
Hyun-Wook Lee Division of Nephrology, Hypertension, and Transplantation, University of Florida College of Medicine, Gainesville, Florida, USA

Search for other papers by Hyun-Wook Lee in
Google Scholar
PubMed Close
,
I David Weiner Division of Nephrology, Hypertension, and Transplantation, University of Florida College of Medicine, Gainesville, Florida, USA
Nephrology and Hypertension Section, North Florida/South Georgia Veterans Health System, Gainesville, Florida, USA

Search for other papers by I David Weiner in
Google Scholar
PubMed Close
,
Yutaka Kakizoe Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Yutaka Kakizoe in
Google Scholar
PubMed Close
,
Takashige Kuwabara Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Takashige Kuwabara in
Google Scholar
PubMed Close
, and
Masashi Mukoyama Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Chuo-ku, Kumamoto, Japan

Search for other papers by Masashi Mukoyama in
Google Scholar
PubMed Close

Rhesus C glycoprotein (Rhcg), an ammonia transporter, is a key molecule in urinary acid excretion and is expressed mainly in the intercalated cells (ICs) of the renal collecting duct. In the present study we investigated the role of aldosterone in the regulation of Rhcg expression. In in vivo experiments using C57BL/6J mice, Western blot analysis showed that continuous subcutaneous administration of aldosterone increased the expression of Rhcg in membrane fraction of the kidney. Supplementation of potassium inhibited the effect of aldosterone on the Rhcg. Next, mice were subjected to adrenalectomy with or without administration of aldosterone, and then ad libitum 0.14 M NH4Cl containing water was given. NH4Cl load increased the expression of Rhcg in membrane fraction. Adrenalectomy decreased NH4Cl-induced Rhcg expression, which was restored by administration of aldosterone. Immunohistochemical studies revealed that NH4Cl load induced the localization of Rhcg at the apical membrane of ICs in the outer medullary collecting duct. Adrenalectomy decreased NH4Cl-induced membrane localization of Rhcg, which was restored by administration of aldosterone. For in vitro experiments, IN-IC cells, an immortalized cell line stably expressing Flag-tagged Rhcg (Rhcg-Flag), were used. Western blot analysis showed that aldosterone increased the expression of Rhcg-Flag in membrane fraction, while the increase in extracellular potassium level inhibited the effect of aldosterone. Both spironolactone and Gӧ6983, a PKC inhibitor, inhibited the expression of Rhcg-Flag in the membrane fraction. These results suggest that aldosterone regulates the membrane expression of Rhcg through the mineralocorticoid receptor and PKC pathways, which is modulated by extracellular potassium level.

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-20-0267
Volume/Issue:
Volume 249: Issue 2
Restricted access