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J. A. KOFOED, C. E. BOZZINI and A. A. TOCCI

Since the work of Watson & Pearce (1949) it has been known that subnormal thyroid activity affects the glycosaminoglycans (GAG) of skin. The changes reported were an increase in the hyaluronic acid and a decrease in the dermatan sulphate content of the skin (Schiller, Slover & Dorfman, 1962; Kofoed & Bozzini, 1967). These results seem to be independent of thyrotrophin because the same changes are noted after hypophysectomy as in hypothyroidism (Schiller et al. 1962). Since hypophysectomy has a different effect on skin and tracheal cartilage GAG (Kofoed & Bozzini, 1969), experiments were done to study the distribution of these compounds in cartilage of thyroidectomized rats and to compare this with the distribution in skin.

Female Wistar rats, weighing approx. 200 g., were used throughout these experiments. Thyroid destruction was accomplished by administration of 700 μc of 131I. Six months after radioactive iodine administration, rats were divided into three

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J. A. KOFOED, A. B. HOUSSAY, A. A. TOCCI, H. M. CURBELO and C. H. GAMPER

SUMMARY

The effects of castration, and of the daily administration of 100 or 300 μg testosterone propionate (TP) for 1 month to castrated animals, on the glycosaminoglycans of the ventral prostate, seminal vesicles and salivary glands, were studied in male white rats.

Castration produced atrophy of the ventral prostate and seminal vesicles together with increased concentration of uronic acid, and atrophy of the submaxillary glands together with decreased uronic acid concentration. The administration of TP to castrated animals produced opposite effects. No changes in weight or in uronic acid concentration were found in the parotid and retrolingual glands. Fractionation on cellulose microcolumns showed that the changes in uronic acid concentration were due to changes in fraction 1 (glycoproteins) in the submaxillary glands, in fractions 2 (hyaluronic acid) and 3 (heparitin sulphate) in the seminal vesicles, and in all the chromatographic fractions in the ventral prostate.